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PCR-based isolation and identification of full-length low-molecular-weight glutenin subunit genes in bread wheat (Triticum aestivum L.).
Theor Appl Genet. 2011 Dec; 123(8):1293-305.TA

Abstract

Low-molecular-weight glutenin subunits (LMW-GSs) are encoded by a multi-gene family and are essential for determining the quality of wheat flour products, such as bread and noodles. However, the exact role or contribution of individual LMW-GS genes to wheat quality remains unclear. This is, at least in part, due to the difficulty in characterizing complete sequences of all LMW-GS gene family members in bread wheat. To identify full-length LMW-GS genes, a polymerase chain reaction (PCR)-based method was established, consisting of newly designed conserved primers and the previously developed LMW-GS gene molecular marker system. Using the PCR-based method, 17 LMW-GS genes were identified and characterized in Xiaoyan 54, of which 12 contained full-length sequences. Sequence alignments showed that 13 LMW-GS genes were identical to those found in Xiaoyan 54 using the genomic DNA library screening, and the other four full-length LMW-GS genes were first isolated from Xiaoyan 54. In Chinese Spring, 16 unique LMW-GS genes were isolated, and 13 of them contained full-length coding sequences. Additionally, 16 and 17 LMW-GS genes in Dongnong 101 and Lvhan 328 (chosen from the micro-core collections of Chinese germplasm), respectively, were also identified. Sequence alignments revealed that at least 15 LMW-GS genes were common in the four wheat varieties, and allelic variants of each gene shared high sequence identities (>95%) but exhibited length polymorphism in repetitive regions. This study provides a PCR-based method for efficiently identifying LMW-GS genes in bread wheat, which will improve the characterization of complex members of the LMW-GS gene family and facilitate the understanding of their contributions to wheat quality.

Authors+Show Affiliations

State Key Laboratory of Plant Cell and Chromosome Engineering, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 1 West Beichen Road, Chaoyang District, Beijing 100101, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21830110

Citation

Zhang, Xiaofei, et al. "PCR-based Isolation and Identification of Full-length Low-molecular-weight Glutenin Subunit Genes in Bread Wheat (Triticum Aestivum L.)." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 123, no. 8, 2011, pp. 1293-305.
Zhang X, Liu D, Jiang W, et al. PCR-based isolation and identification of full-length low-molecular-weight glutenin subunit genes in bread wheat (Triticum aestivum L.). Theor Appl Genet. 2011;123(8):1293-305.
Zhang, X., Liu, D., Jiang, W., Guo, X., Yang, W., Sun, J., Ling, H., & Zhang, A. (2011). PCR-based isolation and identification of full-length low-molecular-weight glutenin subunit genes in bread wheat (Triticum aestivum L.). TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 123(8), 1293-305. https://doi.org/10.1007/s00122-011-1667-8
Zhang X, et al. PCR-based Isolation and Identification of Full-length Low-molecular-weight Glutenin Subunit Genes in Bread Wheat (Triticum Aestivum L.). Theor Appl Genet. 2011;123(8):1293-305. PubMed PMID: 21830110.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - PCR-based isolation and identification of full-length low-molecular-weight glutenin subunit genes in bread wheat (Triticum aestivum L.). AU - Zhang,Xiaofei, AU - Liu,Dongcheng, AU - Jiang,Wei, AU - Guo,Xiaoli, AU - Yang,Wenlong, AU - Sun,Jiazhu, AU - Ling,Hongqing, AU - Zhang,Aimin, Y1 - 2011/08/10/ PY - 2011/04/06/received PY - 2011/07/16/accepted PY - 2011/8/11/entrez PY - 2011/8/11/pubmed PY - 2012/3/1/medline SP - 1293 EP - 305 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 123 IS - 8 N2 - Low-molecular-weight glutenin subunits (LMW-GSs) are encoded by a multi-gene family and are essential for determining the quality of wheat flour products, such as bread and noodles. However, the exact role or contribution of individual LMW-GS genes to wheat quality remains unclear. This is, at least in part, due to the difficulty in characterizing complete sequences of all LMW-GS gene family members in bread wheat. To identify full-length LMW-GS genes, a polymerase chain reaction (PCR)-based method was established, consisting of newly designed conserved primers and the previously developed LMW-GS gene molecular marker system. Using the PCR-based method, 17 LMW-GS genes were identified and characterized in Xiaoyan 54, of which 12 contained full-length sequences. Sequence alignments showed that 13 LMW-GS genes were identical to those found in Xiaoyan 54 using the genomic DNA library screening, and the other four full-length LMW-GS genes were first isolated from Xiaoyan 54. In Chinese Spring, 16 unique LMW-GS genes were isolated, and 13 of them contained full-length coding sequences. Additionally, 16 and 17 LMW-GS genes in Dongnong 101 and Lvhan 328 (chosen from the micro-core collections of Chinese germplasm), respectively, were also identified. Sequence alignments revealed that at least 15 LMW-GS genes were common in the four wheat varieties, and allelic variants of each gene shared high sequence identities (>95%) but exhibited length polymorphism in repetitive regions. This study provides a PCR-based method for efficiently identifying LMW-GS genes in bread wheat, which will improve the characterization of complex members of the LMW-GS gene family and facilitate the understanding of their contributions to wheat quality. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/21830110/PCR_based_isolation_and_identification_of_full_length_low_molecular_weight_glutenin_subunit_genes_in_bread_wheat__Triticum_aestivum_L___ L2 - https://dx.doi.org/10.1007/s00122-011-1667-8 DB - PRIME DP - Unbound Medicine ER -