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Prevalence of plasmid-mediated quinolone resistance and mutations in the gyrase and topoisomerase IV genes in Salmonella isolated from 12 tertiary-care hospitals in Korea.
Microb Drug Resist. 2011 Dec; 17(4):551-7.MD

Abstract

BACKGROUND

The aim of this study was to investigate the prevalence of plasmid-mediated quinolone resistance (PMQR) and mutations in quinolone resistance-determining regions (QRDRs) of Salmonella and their association with fluoroquinolone susceptibility in Korea.

METHODS

A total of 284 nonduplicated clinical isolates of Salmonella were collected from various clinical specimens at 12 tertiary-care hospitals in Korea. The qnrA, qnrB, and qnrS genes were detected by multiplex polymerase chain reaction (PCR). The qepA and aac(6')-Ib-cr genes were amplified by PCR. The QRDRs of gyrA, gyrB, parC, and parE were amplified by PCR from the DNA of selected nalidixic acid-resistant and qnr-positive isolates.

RESULTS

We detected six qnr-positive Salmonella (four qnrS1 and two qnrB19) and one aac(6')-Ib-cr-positive strain. A mutation in the QRDR of gyrA only (N=46) was the most common, followed by gyrA+parC (N=9), parC (N=7), gyrA+parE (N=3), parC+parE (N=3), gyrA+gyrB (N=2), and parE (N=1). There were seven novel mutations in the QRDR regions of gyrB, parC, and parE. Six of seven PMQR-positive isolates had high-level resistance to nalidixic acid, and all six strains had reduced susceptibility to ciprofloxacin. One qnrS1-positive isolate was resistant to ciprofloxacin, norfloxacin, and nalidixic acid. The resistant rates to nalidixic acid, ciprofloxacin, norfloxacin, and levofloxacin were 49.3%, 1.1%, 0.7%, and 0.4%, respectively.

CONCLUSION

We report the first detection of PMQR in Salmonella isolates from Korea. It is essential to continue surveillance and to watch for the spread of PMQR in Salmonella for public health control.

Authors+Show Affiliations

Department of Laboratory Medicine, Inje University College of Medicine, 633-165 Gaegeum-dong Busanjin-gu, Busan, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21830947

Citation

Jeong, Haeng Soon, et al. "Prevalence of Plasmid-mediated Quinolone Resistance and Mutations in the Gyrase and Topoisomerase IV Genes in Salmonella Isolated From 12 Tertiary-care Hospitals in Korea." Microbial Drug Resistance (Larchmont, N.Y.), vol. 17, no. 4, 2011, pp. 551-7.
Jeong HS, Kim JA, Shin JH, et al. Prevalence of plasmid-mediated quinolone resistance and mutations in the gyrase and topoisomerase IV genes in Salmonella isolated from 12 tertiary-care hospitals in Korea. Microb Drug Resist. 2011;17(4):551-7.
Jeong, H. S., Kim, J. A., Shin, J. H., Chang, C. L., Jeong, J., Cho, J. H., Kim, M. N., Kim, S., Kim, Y. R., Lee, C. H., Lee, K., Lee, M. A., Lee, W. G., Shin, J. H., & Lee, J. N. (2011). Prevalence of plasmid-mediated quinolone resistance and mutations in the gyrase and topoisomerase IV genes in Salmonella isolated from 12 tertiary-care hospitals in Korea. Microbial Drug Resistance (Larchmont, N.Y.), 17(4), 551-7. https://doi.org/10.1089/mdr.2011.0095
Jeong HS, et al. Prevalence of Plasmid-mediated Quinolone Resistance and Mutations in the Gyrase and Topoisomerase IV Genes in Salmonella Isolated From 12 Tertiary-care Hospitals in Korea. Microb Drug Resist. 2011;17(4):551-7. PubMed PMID: 21830947.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence of plasmid-mediated quinolone resistance and mutations in the gyrase and topoisomerase IV genes in Salmonella isolated from 12 tertiary-care hospitals in Korea. AU - Jeong,Haeng Soon, AU - Kim,Jeong A, AU - Shin,Jeong Hwan, AU - Chang,Chulhun L, AU - Jeong,Joseph, AU - Cho,Ji-Hyun, AU - Kim,Mi-Na, AU - Kim,Sunjoo, AU - Kim,Young Ree, AU - Lee,Chae Hoon, AU - Lee,Kyungwon, AU - Lee,Mi Ae, AU - Lee,Wee Gyo, AU - Shin,Jong Hee, AU - Lee,Jeong Nyeo, Y1 - 2011/08/10/ PY - 2011/8/12/entrez PY - 2011/8/13/pubmed PY - 2012/3/31/medline SP - 551 EP - 7 JF - Microbial drug resistance (Larchmont, N.Y.) JO - Microb Drug Resist VL - 17 IS - 4 N2 - BACKGROUND: The aim of this study was to investigate the prevalence of plasmid-mediated quinolone resistance (PMQR) and mutations in quinolone resistance-determining regions (QRDRs) of Salmonella and their association with fluoroquinolone susceptibility in Korea. METHODS: A total of 284 nonduplicated clinical isolates of Salmonella were collected from various clinical specimens at 12 tertiary-care hospitals in Korea. The qnrA, qnrB, and qnrS genes were detected by multiplex polymerase chain reaction (PCR). The qepA and aac(6')-Ib-cr genes were amplified by PCR. The QRDRs of gyrA, gyrB, parC, and parE were amplified by PCR from the DNA of selected nalidixic acid-resistant and qnr-positive isolates. RESULTS: We detected six qnr-positive Salmonella (four qnrS1 and two qnrB19) and one aac(6')-Ib-cr-positive strain. A mutation in the QRDR of gyrA only (N=46) was the most common, followed by gyrA+parC (N=9), parC (N=7), gyrA+parE (N=3), parC+parE (N=3), gyrA+gyrB (N=2), and parE (N=1). There were seven novel mutations in the QRDR regions of gyrB, parC, and parE. Six of seven PMQR-positive isolates had high-level resistance to nalidixic acid, and all six strains had reduced susceptibility to ciprofloxacin. One qnrS1-positive isolate was resistant to ciprofloxacin, norfloxacin, and nalidixic acid. The resistant rates to nalidixic acid, ciprofloxacin, norfloxacin, and levofloxacin were 49.3%, 1.1%, 0.7%, and 0.4%, respectively. CONCLUSION: We report the first detection of PMQR in Salmonella isolates from Korea. It is essential to continue surveillance and to watch for the spread of PMQR in Salmonella for public health control. SN - 1931-8448 UR - https://www.unboundmedicine.com/medline/citation/21830947/Prevalence_of_plasmid_mediated_quinolone_resistance_and_mutations_in_the_gyrase_and_topoisomerase_IV_genes_in_Salmonella_isolated_from_12_tertiary_care_hospitals_in_Korea_ L2 - https://www.liebertpub.com/doi/10.1089/mdr.2011.0095?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -