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High performance liquid chromatography-high resolution mass spectrometry and micropulverized extraction for the quantification of amphetamines, cocaine, opioids, benzodiazepines, antidepressants and hallucinogens in 2.5 mg hair samples.
J Chromatogr A. 2011 Sep 23; 1218(38):6583-95.JC

Abstract

A high performance liquid chromatography-high resolution mass spectrometry (HPLC-HRMS) method for simultaneous screening and quantification of 28 drugs was developed and validated for 2.5 mg hair samples. Target drugs and their metabolites included amphetamines, cocaine, opioids, benzodiazepines, antidepressants, and hallucinogens. After decontamination, hair samples were extracted with 200 μL of a mixture of water: acetonitrile:1 M trifluoroacetic acid (80:10:10, v/v) using a 5 min simultaneous pulverization/extraction step. The extracts were analysed by HPLC-HRMS in an Orbitrap at a nominal resolution of 60,000, with concomitant in source collisional experiments (in source CID). Gradient elution on an Atlantis T3 column resolved 28 target compounds and 5 internal standards. Total chromatographic run time was 26 min. Calibration was achieved by linear regression analysis utilizing six calibration points; R2 ranged from 0.9964 to 0.9999, the limits of quantification were 0.1 ng/mg for 8 compounds, 0.2 ng/mg for 16 compounds and 0.5 ng/mg for 4 compounds; mean relative errors from -21% to +23% were obtained; relative standard deviation, used to estimate repeatability and intermediate reproducibility at three concentrations, was always less than 20%. Process efficiency and recoveries for all analytes were better than 65 and 73%, respectively, at any concentration. The method was applied to hair samples from forensic investigations that contained a broad assortment of drugs of abuse and pharmaceuticals. The use of concomitant HRMS full scan and CID afforded the possibility of retrospective analysis for discovering untargeted drugs.

Authors+Show Affiliations

Department of Public Health and Environmental Medicine, University of Padova, and Forensic Toxicology and Antidoping, University Hospital of Padova, Via Falloppio 50, I-35121 Padova, Italy. donata.favretto@unipd.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

21831386

Citation

Favretto, Donata, et al. "High Performance Liquid Chromatography-high Resolution Mass Spectrometry and Micropulverized Extraction for the Quantification of Amphetamines, Cocaine, Opioids, Benzodiazepines, Antidepressants and Hallucinogens in 2.5 Mg Hair Samples." Journal of Chromatography. A, vol. 1218, no. 38, 2011, pp. 6583-95.
Favretto D, Vogliardi S, Stocchero G, et al. High performance liquid chromatography-high resolution mass spectrometry and micropulverized extraction for the quantification of amphetamines, cocaine, opioids, benzodiazepines, antidepressants and hallucinogens in 2.5 mg hair samples. J Chromatogr A. 2011;1218(38):6583-95.
Favretto, D., Vogliardi, S., Stocchero, G., Nalesso, A., Tucci, M., & Ferrara, S. D. (2011). High performance liquid chromatography-high resolution mass spectrometry and micropulverized extraction for the quantification of amphetamines, cocaine, opioids, benzodiazepines, antidepressants and hallucinogens in 2.5 mg hair samples. Journal of Chromatography. A, 1218(38), 6583-95. https://doi.org/10.1016/j.chroma.2011.07.050
Favretto D, et al. High Performance Liquid Chromatography-high Resolution Mass Spectrometry and Micropulverized Extraction for the Quantification of Amphetamines, Cocaine, Opioids, Benzodiazepines, Antidepressants and Hallucinogens in 2.5 Mg Hair Samples. J Chromatogr A. 2011 Sep 23;1218(38):6583-95. PubMed PMID: 21831386.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High performance liquid chromatography-high resolution mass spectrometry and micropulverized extraction for the quantification of amphetamines, cocaine, opioids, benzodiazepines, antidepressants and hallucinogens in 2.5 mg hair samples. AU - Favretto,Donata, AU - Vogliardi,Susanna, AU - Stocchero,Giulia, AU - Nalesso,Alessandro, AU - Tucci,Marianna, AU - Ferrara,Santo Davide, Y1 - 2011/07/22/ PY - 2011/04/04/received PY - 2011/06/28/revised PY - 2011/07/14/accepted PY - 2011/8/12/entrez PY - 2011/8/13/pubmed PY - 2011/11/4/medline SP - 6583 EP - 95 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1218 IS - 38 N2 - A high performance liquid chromatography-high resolution mass spectrometry (HPLC-HRMS) method for simultaneous screening and quantification of 28 drugs was developed and validated for 2.5 mg hair samples. Target drugs and their metabolites included amphetamines, cocaine, opioids, benzodiazepines, antidepressants, and hallucinogens. After decontamination, hair samples were extracted with 200 μL of a mixture of water: acetonitrile:1 M trifluoroacetic acid (80:10:10, v/v) using a 5 min simultaneous pulverization/extraction step. The extracts were analysed by HPLC-HRMS in an Orbitrap at a nominal resolution of 60,000, with concomitant in source collisional experiments (in source CID). Gradient elution on an Atlantis T3 column resolved 28 target compounds and 5 internal standards. Total chromatographic run time was 26 min. Calibration was achieved by linear regression analysis utilizing six calibration points; R2 ranged from 0.9964 to 0.9999, the limits of quantification were 0.1 ng/mg for 8 compounds, 0.2 ng/mg for 16 compounds and 0.5 ng/mg for 4 compounds; mean relative errors from -21% to +23% were obtained; relative standard deviation, used to estimate repeatability and intermediate reproducibility at three concentrations, was always less than 20%. Process efficiency and recoveries for all analytes were better than 65 and 73%, respectively, at any concentration. The method was applied to hair samples from forensic investigations that contained a broad assortment of drugs of abuse and pharmaceuticals. The use of concomitant HRMS full scan and CID afforded the possibility of retrospective analysis for discovering untargeted drugs. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/21831386/High_performance_liquid_chromatography_high_resolution_mass_spectrometry_and_micropulverized_extraction_for_the_quantification_of_amphetamines_cocaine_opioids_benzodiazepines_antidepressants_and_hallucinogens_in_2_5_mg_hair_samples_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(11)01060-0 DB - PRIME DP - Unbound Medicine ER -