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Recombinant human erythropoietin prevents cisplatin-induced genotoxicity in rat liver and heart tissues via an antioxidant process.
Drug Chem Toxicol. 2012 Apr; 35(2):134-40.DC

Abstract

Cisplatin (Cisp) is one of the most effective chemotherapeutic agents. However, at higher doses, liver and heart injuries may occur. Recombinant human erythropoietin (rhEPO) has recently been shown to exert an important cytoprotective effect in many tissues. For that reason, we tried to check the protective effect of rhEPO against Cisp-induced genotoxicity and oxidative stress in liver and heart tissues. Our experiments were performed using six groups of adult male Wistar rats. The control group was treated only with saline solution. The rhEPO group was given a single dose of rhEPO. The Cisp group was given a single injection of Cisp. The rhEPO+Cisp groups were given rhEPO simultaneously, 24 hours before, and 5 days after Cisp injection. Our results clearly showed that Cisp induced noticeable DNA damage in the liver and heart, accompanied by a significant increase in protein carbonyl level, reduced glutathione (GSH) depletion, and a decrease in catalase activity. Rats treated with rhEPO, simultaneously, before, or after Cisp injection, remarkably decreased DNA damage. It decreased also the protein carbonyl level, restored GSH depletion, and enhanced catalase activity. Our results highlight an interesting cytoprotective strategy using rhEPO against Cisp-induced liver and heart injuries.

Authors+Show Affiliations

Laboratory of Research on Biologically Compatible Compounds, Faculty of Dentistry, Monastir, Tunisia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21834696

Citation

Rjiba-Touati, Karima, et al. "Recombinant Human Erythropoietin Prevents Cisplatin-induced Genotoxicity in Rat Liver and Heart Tissues Via an Antioxidant Process." Drug and Chemical Toxicology, vol. 35, no. 2, 2012, pp. 134-40.
Rjiba-Touati K, Ayed-Boussema I, Belarbia A, et al. Recombinant human erythropoietin prevents cisplatin-induced genotoxicity in rat liver and heart tissues via an antioxidant process. Drug Chem Toxicol. 2012;35(2):134-40.
Rjiba-Touati, K., Ayed-Boussema, I., Belarbia, A., Achour, A., & Bacha, H. (2012). Recombinant human erythropoietin prevents cisplatin-induced genotoxicity in rat liver and heart tissues via an antioxidant process. Drug and Chemical Toxicology, 35(2), 134-40. https://doi.org/10.3109/01480545.2011.589445
Rjiba-Touati K, et al. Recombinant Human Erythropoietin Prevents Cisplatin-induced Genotoxicity in Rat Liver and Heart Tissues Via an Antioxidant Process. Drug Chem Toxicol. 2012;35(2):134-40. PubMed PMID: 21834696.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Recombinant human erythropoietin prevents cisplatin-induced genotoxicity in rat liver and heart tissues via an antioxidant process. AU - Rjiba-Touati,Karima, AU - Ayed-Boussema,Imen, AU - Belarbia,Anis, AU - Achour,Abdelatif, AU - Bacha,Hassen, Y1 - 2011/08/11/ PY - 2011/8/13/entrez PY - 2011/8/13/pubmed PY - 2012/9/12/medline SP - 134 EP - 40 JF - Drug and chemical toxicology JO - Drug Chem Toxicol VL - 35 IS - 2 N2 - Cisplatin (Cisp) is one of the most effective chemotherapeutic agents. However, at higher doses, liver and heart injuries may occur. Recombinant human erythropoietin (rhEPO) has recently been shown to exert an important cytoprotective effect in many tissues. For that reason, we tried to check the protective effect of rhEPO against Cisp-induced genotoxicity and oxidative stress in liver and heart tissues. Our experiments were performed using six groups of adult male Wistar rats. The control group was treated only with saline solution. The rhEPO group was given a single dose of rhEPO. The Cisp group was given a single injection of Cisp. The rhEPO+Cisp groups were given rhEPO simultaneously, 24 hours before, and 5 days after Cisp injection. Our results clearly showed that Cisp induced noticeable DNA damage in the liver and heart, accompanied by a significant increase in protein carbonyl level, reduced glutathione (GSH) depletion, and a decrease in catalase activity. Rats treated with rhEPO, simultaneously, before, or after Cisp injection, remarkably decreased DNA damage. It decreased also the protein carbonyl level, restored GSH depletion, and enhanced catalase activity. Our results highlight an interesting cytoprotective strategy using rhEPO against Cisp-induced liver and heart injuries. SN - 1525-6014 UR - https://www.unboundmedicine.com/medline/citation/21834696/Recombinant_human_erythropoietin_prevents_cisplatin_induced_genotoxicity_in_rat_liver_and_heart_tissues_via_an_antioxidant_process_ L2 - https://www.tandfonline.com/doi/full/10.3109/01480545.2011.589445 DB - PRIME DP - Unbound Medicine ER -