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Substrate-accelerated death of Saccharomyces cerevisiae CBS 8066 under maltose stress.
Yeast. 1990 Mar-Apr; 6(2):149-58.Y

Abstract

When Saccharomyces cerevisiae CBS 8066 was grown under maltose limitation, two enzymes specific for maltose utilization were present: a maltose carrier, and the maltose-hydrolysing alpha-glucosidase. The role of these two enzymes in the physiology of S. cerevisiae was investigated in a comparative study in which Candida utilis CBS 621 was used as a reference organism. Maltose pulses to a maltose-limited chemostat culture of S. cerevisiae resulted in 'substrate-accelerated death'. This was evident from: (1) enhanced protein release from cells; (2) excretion of glucose into the medium; (3) decreased viability. These effects wee specific with respect to both substrate and organism: pulses of glucose to maltose-limited cultures of S. cerevisiae did not result in cell death, neither did maltose pulses to maltose-limited cultures of C. utilis. The maltose-accelerated death of s. cerevisiae is most likely explained in terms of an uncontrolled uptake of maltose into the cell, resulting in an osmotic burst. Our results also provide evidence that the aerobic alcoholic fermentation that occurs after pulsing sugars to sugar-limited cultures of s. cerevisiae (short-term Crabtree effect) cannot solely be explained in terms of the mechanism of sugar transport. Both glucose and maltose pulses to maltose-limited cultures triggered aerobic alcohol formation. However, glucose transport by S. cerevisiae occurs via facilitated diffusion, whereas maltose entry into this yeast is mediated by a maltose/proton symport system.

Authors+Show Affiliations

Postma E
Department of Microbiology and Enzymology, Delft University of Technology, The Netherlands.
Verduyn C
No affiliation info available
Kuiper A
No affiliation info available
Scheffers WA
No affiliation info available
van Dijken JP
No affiliation info available

MeSH

Carrier ProteinsCulture MediaGlucoseHydrogen-Ion ConcentrationMaltoseMaltose-Binding ProteinsSaccharomyces cerevisiaealpha-Glucosidases

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2183522

Citation

Postma, E, et al. "Substrate-accelerated Death of Saccharomyces Cerevisiae CBS 8066 Under Maltose Stress." Yeast (Chichester, England), vol. 6, no. 2, 1990, pp. 149-58.
Postma E, Verduyn C, Kuiper A, et al. Substrate-accelerated death of Saccharomyces cerevisiae CBS 8066 under maltose stress. Yeast. 1990;6(2):149-58.
Postma, E., Verduyn, C., Kuiper, A., Scheffers, W. A., & van Dijken, J. P. (1990). Substrate-accelerated death of Saccharomyces cerevisiae CBS 8066 under maltose stress. Yeast (Chichester, England), 6(2), 149-58.
Postma E, et al. Substrate-accelerated Death of Saccharomyces Cerevisiae CBS 8066 Under Maltose Stress. Yeast. 1990 Mar-Apr;6(2):149-58. PubMed PMID: 2183522.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Substrate-accelerated death of Saccharomyces cerevisiae CBS 8066 under maltose stress. AU - Postma,E, AU - Verduyn,C, AU - Kuiper,A, AU - Scheffers,W A, AU - van Dijken,J P, PY - 1990/3/1/pubmed PY - 1990/3/1/medline PY - 1990/3/1/entrez SP - 149 EP - 58 JF - Yeast (Chichester, England) JO - Yeast VL - 6 IS - 2 N2 - When Saccharomyces cerevisiae CBS 8066 was grown under maltose limitation, two enzymes specific for maltose utilization were present: a maltose carrier, and the maltose-hydrolysing alpha-glucosidase. The role of these two enzymes in the physiology of S. cerevisiae was investigated in a comparative study in which Candida utilis CBS 621 was used as a reference organism. Maltose pulses to a maltose-limited chemostat culture of S. cerevisiae resulted in 'substrate-accelerated death'. This was evident from: (1) enhanced protein release from cells; (2) excretion of glucose into the medium; (3) decreased viability. These effects wee specific with respect to both substrate and organism: pulses of glucose to maltose-limited cultures of S. cerevisiae did not result in cell death, neither did maltose pulses to maltose-limited cultures of C. utilis. The maltose-accelerated death of s. cerevisiae is most likely explained in terms of an uncontrolled uptake of maltose into the cell, resulting in an osmotic burst. Our results also provide evidence that the aerobic alcoholic fermentation that occurs after pulsing sugars to sugar-limited cultures of s. cerevisiae (short-term Crabtree effect) cannot solely be explained in terms of the mechanism of sugar transport. Both glucose and maltose pulses to maltose-limited cultures triggered aerobic alcohol formation. However, glucose transport by S. cerevisiae occurs via facilitated diffusion, whereas maltose entry into this yeast is mediated by a maltose/proton symport system. SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/2183522/Substrate_accelerated_death_of_Saccharomyces_cerevisiae_CBS_8066_under_maltose_stress_ DB - PRIME DP - Unbound Medicine ER -