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Catalytic and thermodynamic properties of a tannase produced by Aspergillus niger GH1 grown on polyurethane foam.
Appl Biochem Biotechnol. 2011 Nov; 165(5-6):1141-51.AB

Abstract

Tannase is an inducible enzyme with important applications in the food and pharmaceutical industries. This enzyme was produced by the fungus Aspergillus niger GH1 under solid-state fermentation using polyurethane foam as solid support and tannic acid as sole carbon source and tannase inducer. Physicochemical properties of A. niger tannase were characterized, and the kinetic and thermodynamics parameters on methyl gallate hydrolysis were evaluated. The enzyme was stable in a pH range of 2-8 and a functional temperature range of 25-65 °C. The highest k(cat) value was 2,611.10 s(-1) at 65 °C. Tannase had more affinity for methyl gallate at 45 °C with a K(M) value of 1.82 mM and an efficiency of hydrolysis (k(cat)/K(M)) of 330.01 s(-1) mM(-1). The lowest E(a) value was found to be 21.38 kJ/mol at 4.4 mM of methyl gallate. The lowest free energy of Gibbs (ΔG) and enthalpy (ΔH) were found to be 64.86 and 18.56 kJ/mol, respectively. Entropy (ΔS) was -0.22 kJ/mol K. Results suggest that the A. niger GH1 tannase is an attractive enzyme for industrial applications due its catalytic and thermodynamical properties.

Authors+Show Affiliations

Department of Food Science and Technology, School of Chemistry, Universidad Autónoma de Coahuila, Saltillo, Coahuila, Mexico.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21837378

Citation

Ramos, Erika L., et al. "Catalytic and Thermodynamic Properties of a Tannase Produced By Aspergillus Niger GH1 Grown On Polyurethane Foam." Applied Biochemistry and Biotechnology, vol. 165, no. 5-6, 2011, pp. 1141-51.
Ramos EL, Mata-Gómez MA, Rodríguez-Durán LV, et al. Catalytic and thermodynamic properties of a tannase produced by Aspergillus niger GH1 grown on polyurethane foam. Appl Biochem Biotechnol. 2011;165(5-6):1141-51.
Ramos, E. L., Mata-Gómez, M. A., Rodríguez-Durán, L. V., Belmares, R. E., Rodríguez-Herrera, R., & Aguilar, C. N. (2011). Catalytic and thermodynamic properties of a tannase produced by Aspergillus niger GH1 grown on polyurethane foam. Applied Biochemistry and Biotechnology, 165(5-6), 1141-51. https://doi.org/10.1007/s12010-011-9331-y
Ramos EL, et al. Catalytic and Thermodynamic Properties of a Tannase Produced By Aspergillus Niger GH1 Grown On Polyurethane Foam. Appl Biochem Biotechnol. 2011;165(5-6):1141-51. PubMed PMID: 21837378.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Catalytic and thermodynamic properties of a tannase produced by Aspergillus niger GH1 grown on polyurethane foam. AU - Ramos,Erika L, AU - Mata-Gómez,Marco A, AU - Rodríguez-Durán,Luis V, AU - Belmares,Ruth E, AU - Rodríguez-Herrera,Raúl, AU - Aguilar,Cristóbal Noe, Y1 - 2011/08/12/ PY - 2011/02/28/received PY - 2011/07/27/accepted PY - 2011/8/13/entrez PY - 2011/8/13/pubmed PY - 2012/3/10/medline SP - 1141 EP - 51 JF - Applied biochemistry and biotechnology JO - Appl. Biochem. Biotechnol. VL - 165 IS - 5-6 N2 - Tannase is an inducible enzyme with important applications in the food and pharmaceutical industries. This enzyme was produced by the fungus Aspergillus niger GH1 under solid-state fermentation using polyurethane foam as solid support and tannic acid as sole carbon source and tannase inducer. Physicochemical properties of A. niger tannase were characterized, and the kinetic and thermodynamics parameters on methyl gallate hydrolysis were evaluated. The enzyme was stable in a pH range of 2-8 and a functional temperature range of 25-65 °C. The highest k(cat) value was 2,611.10 s(-1) at 65 °C. Tannase had more affinity for methyl gallate at 45 °C with a K(M) value of 1.82 mM and an efficiency of hydrolysis (k(cat)/K(M)) of 330.01 s(-1) mM(-1). The lowest E(a) value was found to be 21.38 kJ/mol at 4.4 mM of methyl gallate. The lowest free energy of Gibbs (ΔG) and enthalpy (ΔH) were found to be 64.86 and 18.56 kJ/mol, respectively. Entropy (ΔS) was -0.22 kJ/mol K. Results suggest that the A. niger GH1 tannase is an attractive enzyme for industrial applications due its catalytic and thermodynamical properties. SN - 1559-0291 UR - https://www.unboundmedicine.com/medline/citation/21837378/Catalytic_and_thermodynamic_properties_of_a_tannase_produced_by_Aspergillus_niger_GH1_grown_on_polyurethane_foam_ L2 - https://dx.doi.org/10.1007/s12010-011-9331-y DB - PRIME DP - Unbound Medicine ER -