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Determination of ofloxacin and lomefloxacin in chicken muscle using molecularly imprinted solid-phase extraction coupled with liquid chromatography.
J Sep Sci. 2011 Oct; 34(19):2668-73.JS

Abstract

A new molecularly imprinted solid-phase extraction (MISPE) procedure combined with liquid chromatography was developed for the simultaneous selective extraction and determination of ofloxacin (OFL) and lomefloxacin (LOM) in chicken muscle samples. The water-compatible molecularly imprinted microspheres (MIMs) were synthesized by aqueous suspension polymerization using 2-hydroxy-3-naphthoic acid and 1-methylpiperazine as mimic templates. The MIMs applied as selective sorbents in SPE method showed high selectivity and affinity to OFL and LOM in complex biological matrices. Good linearity was obtained in a range of 0.025-2.0 μg/g, and the average recoveries of OFL and LOM at three spiked levels ranged from 94.4 to 96.9%, respectively, with the relative standard deviation ≤4.7%. The developed MISPE-HPLC method was successfully applied to the isolation of OFL and LOM in chicken muscles, which demonstrated the potential ability of the novel MIMs for selective extraction of fluoroquinolones in biological samples.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Qiao J
Key Laboratory of Pharmaceutical Quality Control of Hebei Province and College of Pharmacy, Hebei University, Baoding, P. R. China.
Yan H
No affiliation info available
Wang H
No affiliation info available
Lv Y
No affiliation info available

MeSH

AdsorptionAnimalsAnti-Bacterial AgentsChickensChromatography, High Pressure LiquidFluoroquinolonesFood ContaminationMeatMolecular ImprintingMusclesOfloxacinPolymersSolid Phase Extraction

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21837624

Citation

Qiao, Jindong, et al. "Determination of Ofloxacin and Lomefloxacin in Chicken Muscle Using Molecularly Imprinted Solid-phase Extraction Coupled With Liquid Chromatography." Journal of Separation Science, vol. 34, no. 19, 2011, pp. 2668-73.
Qiao J, Yan H, Wang H, et al. Determination of ofloxacin and lomefloxacin in chicken muscle using molecularly imprinted solid-phase extraction coupled with liquid chromatography. J Sep Sci. 2011;34(19):2668-73.
Qiao, J., Yan, H., Wang, H., & Lv, Y. (2011). Determination of ofloxacin and lomefloxacin in chicken muscle using molecularly imprinted solid-phase extraction coupled with liquid chromatography. Journal of Separation Science, 34(19), 2668-73. https://doi.org/10.1002/jssc.201100209
Qiao J, et al. Determination of Ofloxacin and Lomefloxacin in Chicken Muscle Using Molecularly Imprinted Solid-phase Extraction Coupled With Liquid Chromatography. J Sep Sci. 2011;34(19):2668-73. PubMed PMID: 21837624.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of ofloxacin and lomefloxacin in chicken muscle using molecularly imprinted solid-phase extraction coupled with liquid chromatography. AU - Qiao,Jindong, AU - Yan,Hongyuan, AU - Wang,Hui, AU - Lv,Yunkai, Y1 - 2011/08/12/ PY - 2011/03/07/received PY - 2011/07/04/revised PY - 2011/07/06/accepted PY - 2011/8/13/entrez PY - 2011/8/13/pubmed PY - 2012/1/12/medline SP - 2668 EP - 73 JF - Journal of separation science JO - J Sep Sci VL - 34 IS - 19 N2 - A new molecularly imprinted solid-phase extraction (MISPE) procedure combined with liquid chromatography was developed for the simultaneous selective extraction and determination of ofloxacin (OFL) and lomefloxacin (LOM) in chicken muscle samples. The water-compatible molecularly imprinted microspheres (MIMs) were synthesized by aqueous suspension polymerization using 2-hydroxy-3-naphthoic acid and 1-methylpiperazine as mimic templates. The MIMs applied as selective sorbents in SPE method showed high selectivity and affinity to OFL and LOM in complex biological matrices. Good linearity was obtained in a range of 0.025-2.0 μg/g, and the average recoveries of OFL and LOM at three spiked levels ranged from 94.4 to 96.9%, respectively, with the relative standard deviation ≤4.7%. The developed MISPE-HPLC method was successfully applied to the isolation of OFL and LOM in chicken muscles, which demonstrated the potential ability of the novel MIMs for selective extraction of fluoroquinolones in biological samples. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/21837624/Determination_of_ofloxacin_and_lomefloxacin_in_chicken_muscle_using_molecularly_imprinted_solid_phase_extraction_coupled_with_liquid_chromatography_ L2 - https://doi.org/10.1002/jssc.201100209 DB - PRIME DP - Unbound Medicine ER -