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Label-free electrochemical aptasensor for thrombin detection with platinum nanoparticles and blocking reagent horseradish peroxidase as enhancers.
Biotechnol Appl Biochem. 2011 Jul-Aug; 58(4):256-60.BA

Abstract

A sensitive label-free electrochemical aptasensor was successfully fabricated for thrombin detection with platinum nanoparticles (Pt) and blocking reagent horseradish peroxidase (HRP) as enhancers. A Nafion®-graphene-coated electrode was first modified with an electrochemical probe of methylene blue (MB) through electrostatic interaction. Then Pt was electrodeposited onto an electrode for immobilization of the thrombin aptamer (TBA). Subsequently, HRP served as blocking reagent instead of bovine serum albumin (BSA). With the synergistic effect between Pt and HRP, the prepared aptasensor showed a superior catalytic efficiency toward H(2) O(2) in the presence of MB. After the combination of target thrombin on electrode surface, the TBA-thrombin complex made a barrier for electrocatalysis of Pt and HRP and inhibited the electrotransfer, resulting in a greater decrease in MB signals. As a result, the proposed approach showed a high sensitivity and a much wider linearity to thrombin in the range from 0.005 to 50 nM with a detection limit of 1 pM.

Authors+Show Affiliations

College of Chemistry and Chemical Engineering, Yibin University, Sichuan, Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21838800

Citation

Sun, Tao, et al. "Label-free Electrochemical Aptasensor for Thrombin Detection With Platinum Nanoparticles and Blocking Reagent Horseradish Peroxidase as Enhancers." Biotechnology and Applied Biochemistry, vol. 58, no. 4, 2011, pp. 256-60.
Sun T, Wang L, Li N, et al. Label-free electrochemical aptasensor for thrombin detection with platinum nanoparticles and blocking reagent horseradish peroxidase as enhancers. Biotechnol Appl Biochem. 2011;58(4):256-60.
Sun, T., Wang, L., Li, N., & Gan, X. (2011). Label-free electrochemical aptasensor for thrombin detection with platinum nanoparticles and blocking reagent horseradish peroxidase as enhancers. Biotechnology and Applied Biochemistry, 58(4), 256-60. https://doi.org/10.1002/bab.33
Sun T, et al. Label-free Electrochemical Aptasensor for Thrombin Detection With Platinum Nanoparticles and Blocking Reagent Horseradish Peroxidase as Enhancers. Biotechnol Appl Biochem. 2011 Jul-Aug;58(4):256-60. PubMed PMID: 21838800.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Label-free electrochemical aptasensor for thrombin detection with platinum nanoparticles and blocking reagent horseradish peroxidase as enhancers. AU - Sun,Tao, AU - Wang,Li, AU - Li,Na, AU - Gan,Xianxue, Y1 - 2011/07/27/ PY - 2011/03/08/received PY - 2011/04/21/accepted PY - 2011/8/16/entrez PY - 2011/8/16/pubmed PY - 2011/12/13/medline SP - 256 EP - 60 JF - Biotechnology and applied biochemistry JO - Biotechnol Appl Biochem VL - 58 IS - 4 N2 - A sensitive label-free electrochemical aptasensor was successfully fabricated for thrombin detection with platinum nanoparticles (Pt) and blocking reagent horseradish peroxidase (HRP) as enhancers. A Nafion®-graphene-coated electrode was first modified with an electrochemical probe of methylene blue (MB) through electrostatic interaction. Then Pt was electrodeposited onto an electrode for immobilization of the thrombin aptamer (TBA). Subsequently, HRP served as blocking reagent instead of bovine serum albumin (BSA). With the synergistic effect between Pt and HRP, the prepared aptasensor showed a superior catalytic efficiency toward H(2) O(2) in the presence of MB. After the combination of target thrombin on electrode surface, the TBA-thrombin complex made a barrier for electrocatalysis of Pt and HRP and inhibited the electrotransfer, resulting in a greater decrease in MB signals. As a result, the proposed approach showed a high sensitivity and a much wider linearity to thrombin in the range from 0.005 to 50 nM with a detection limit of 1 pM. SN - 1470-8744 UR - https://www.unboundmedicine.com/medline/citation/21838800/Label_free_electrochemical_aptasensor_for_thrombin_detection_with_platinum_nanoparticles_and_blocking_reagent_horseradish_peroxidase_as_enhancers_ L2 - https://doi.org/10.1002/bab.33 DB - PRIME DP - Unbound Medicine ER -