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Maslinic acid, a natural triterpenoid compound from Olea europaea, protects cortical neurons against oxygen-glucose deprivation-induced injury.
Eur J Pharmacol. 2011 Nov 16; 670(1):148-53.EJ

Abstract

Maslinic acid is a triterpenoid compound present in plants of Olea europaea. This compound has been reported to have potent antioxidant, anti-cancer, anti-HIV and anti-inflammatory activities. In this study, we investigated the neuroprotective effect of maslinic acid and its mechanism of action. With presence or absence of maslinic acid, cortical neurons were subjected to 1h of oxygen-glucose deprivation and 24h of reoxygenation. Cell injury was determined by lactate dehydrogenase (LDH) measurement and 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. Neuronal apoptosis was evaluated by flow cytometry assay, caspase-3 expression/activity, caspase-9 activity and Bcl-2/Bax ratio. Nitric Oxide (NO) production and inducible nitric oxide synthase (iNOS) expression were also detected. Results showed that maslinic acid dose-dependently ameliorated neuron injury and apoptosis. Maslinic acid treatment normalized the caspase expression/activation and increased the Bcl-2/Bax ratio. In addition, maslinic acid inhibited oxygen-glucose deprivation-induced NO production and iNOS expression. These results indicated that maslinic acid has beneficial effects on hypoxic neurons by suppressing iNOS activation, which may, in turn, provide neuroprotection.

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Authors+Show Affiliations

Qian Y
Department of Physiology, China Pharmaceutical University, 24 Tongjiaxiang Street, Nanjing 210009, PR China.
Guan T
No affiliation info available
Tang X
No affiliation info available
Huang L
No affiliation info available
Huang M
No affiliation info available
Li Y
No affiliation info available
Sun H
No affiliation info available

MeSH

AnimalsApoptosisBiological ProductsCaspase 3Cerebral CortexGene Expression Regulation, EnzymologicGlucoseIschemiaNeuronsNitric OxideNitric Oxide Synthase Type IIOleaOxygenProto-Oncogene Proteins c-bcl-2RatsRats, Sprague-DawleyTriterpenesbcl-2-Associated X Protein

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21839077

Citation

Qian, Yisong, et al. "Maslinic Acid, a Natural Triterpenoid Compound From Olea Europaea, Protects Cortical Neurons Against Oxygen-glucose Deprivation-induced Injury." European Journal of Pharmacology, vol. 670, no. 1, 2011, pp. 148-53.
Qian Y, Guan T, Tang X, et al. Maslinic acid, a natural triterpenoid compound from Olea europaea, protects cortical neurons against oxygen-glucose deprivation-induced injury. Eur J Pharmacol. 2011;670(1):148-53.
Qian, Y., Guan, T., Tang, X., Huang, L., Huang, M., Li, Y., & Sun, H. (2011). Maslinic acid, a natural triterpenoid compound from Olea europaea, protects cortical neurons against oxygen-glucose deprivation-induced injury. European Journal of Pharmacology, 670(1), 148-53. https://doi.org/10.1016/j.ejphar.2011.07.037
Qian Y, et al. Maslinic Acid, a Natural Triterpenoid Compound From Olea Europaea, Protects Cortical Neurons Against Oxygen-glucose Deprivation-induced Injury. Eur J Pharmacol. 2011 Nov 16;670(1):148-53. PubMed PMID: 21839077.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Maslinic acid, a natural triterpenoid compound from Olea europaea, protects cortical neurons against oxygen-glucose deprivation-induced injury. AU - Qian,Yisong, AU - Guan,Teng, AU - Tang,Xuzhen, AU - Huang,Longfei, AU - Huang,Menghao, AU - Li,Yunman, AU - Sun,Hongbin, Y1 - 2011/08/05/ PY - 2011/02/24/received PY - 2011/06/17/revised PY - 2011/07/24/accepted PY - 2011/8/16/entrez PY - 2011/8/16/pubmed PY - 2012/2/7/medline SP - 148 EP - 53 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 670 IS - 1 N2 - Maslinic acid is a triterpenoid compound present in plants of Olea europaea. This compound has been reported to have potent antioxidant, anti-cancer, anti-HIV and anti-inflammatory activities. In this study, we investigated the neuroprotective effect of maslinic acid and its mechanism of action. With presence or absence of maslinic acid, cortical neurons were subjected to 1h of oxygen-glucose deprivation and 24h of reoxygenation. Cell injury was determined by lactate dehydrogenase (LDH) measurement and 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. Neuronal apoptosis was evaluated by flow cytometry assay, caspase-3 expression/activity, caspase-9 activity and Bcl-2/Bax ratio. Nitric Oxide (NO) production and inducible nitric oxide synthase (iNOS) expression were also detected. Results showed that maslinic acid dose-dependently ameliorated neuron injury and apoptosis. Maslinic acid treatment normalized the caspase expression/activation and increased the Bcl-2/Bax ratio. In addition, maslinic acid inhibited oxygen-glucose deprivation-induced NO production and iNOS expression. These results indicated that maslinic acid has beneficial effects on hypoxic neurons by suppressing iNOS activation, which may, in turn, provide neuroprotection. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/21839077/Maslinic_acid_a_natural_triterpenoid_compound_from_Olea_europaea_protects_cortical_neurons_against_oxygen_glucose_deprivation_induced_injury_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=21839077.ui DB - PRIME DP - Unbound Medicine ER -