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Evaluation of Salmonella enterica serovar Typhimurium and Choleraesuis slyA mutant strains for use in live attenuated oral vaccines.
Comp Immunol Microbiol Infect Dis. 2011 Sep; 34(5):399-409.CI

Abstract

SlyA protein plays a key role in virulence in Salmonella enterica. In this study, we evaluated the ability of the slyA mutant strain of S. enterica serovar Choleraesuis (S. choleraesuis) to protect against swine salmonellosis. Using a murine model infected with S. enterica serovar Typhimurium (S. typhimurium), we showed that the Salmonella strain with a deletion of slyA could be used as a highly immunogenic, effective and safe vaccine in mice. Based on these data, a slyA mutant of S. enterica serovar Choleraesuis strain RF-1 was constructed, and the ability of this mutant to protect immunized pigs from S. choleraesuis infection was examined. As with the S. typhimurium slyA mutant, immunization of pigs with the S. choleraesuis slyA mutant strain provided significant protection against subsequent challenge by the wild-type RF-1. These results demonstrate that SlyA is a potential target in the development of a novel live attenuated vaccine against S. enterica.

Authors+Show Affiliations

Department of Microbiology, School of Pharmacy, Kitasato University, Minato-ku, Tokyo 108-8641, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial

Language

eng

PubMed ID

21840053

Citation

Haneda, Takeshi, et al. "Evaluation of Salmonella Enterica Serovar Typhimurium and Choleraesuis slyA Mutant Strains for Use in Live Attenuated Oral Vaccines." Comparative Immunology, Microbiology and Infectious Diseases, vol. 34, no. 5, 2011, pp. 399-409.
Haneda T, Okada N, Kikuchi Y, et al. Evaluation of Salmonella enterica serovar Typhimurium and Choleraesuis slyA mutant strains for use in live attenuated oral vaccines. Comp Immunol Microbiol Infect Dis. 2011;34(5):399-409.
Haneda, T., Okada, N., Kikuchi, Y., Takagi, M., Kurotaki, T., Miki, T., Arai, S., & Danbara, H. (2011). Evaluation of Salmonella enterica serovar Typhimurium and Choleraesuis slyA mutant strains for use in live attenuated oral vaccines. Comparative Immunology, Microbiology and Infectious Diseases, 34(5), 399-409. https://doi.org/10.1016/j.cimid.2011.07.001
Haneda T, et al. Evaluation of Salmonella Enterica Serovar Typhimurium and Choleraesuis slyA Mutant Strains for Use in Live Attenuated Oral Vaccines. Comp Immunol Microbiol Infect Dis. 2011;34(5):399-409. PubMed PMID: 21840053.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of Salmonella enterica serovar Typhimurium and Choleraesuis slyA mutant strains for use in live attenuated oral vaccines. AU - Haneda,Takeshi, AU - Okada,Nobuhiko, AU - Kikuchi,Yuji, AU - Takagi,Masami, AU - Kurotaki,Tetsuro, AU - Miki,Tsuyoshi, AU - Arai,Setsuo, AU - Danbara,Hirofumi, Y1 - 2011/08/12/ PY - 2011/01/19/received PY - 2011/07/05/revised PY - 2011/07/19/accepted PY - 2011/8/16/entrez PY - 2011/8/16/pubmed PY - 2012/1/10/medline SP - 399 EP - 409 JF - Comparative immunology, microbiology and infectious diseases JO - Comp Immunol Microbiol Infect Dis VL - 34 IS - 5 N2 - SlyA protein plays a key role in virulence in Salmonella enterica. In this study, we evaluated the ability of the slyA mutant strain of S. enterica serovar Choleraesuis (S. choleraesuis) to protect against swine salmonellosis. Using a murine model infected with S. enterica serovar Typhimurium (S. typhimurium), we showed that the Salmonella strain with a deletion of slyA could be used as a highly immunogenic, effective and safe vaccine in mice. Based on these data, a slyA mutant of S. enterica serovar Choleraesuis strain RF-1 was constructed, and the ability of this mutant to protect immunized pigs from S. choleraesuis infection was examined. As with the S. typhimurium slyA mutant, immunization of pigs with the S. choleraesuis slyA mutant strain provided significant protection against subsequent challenge by the wild-type RF-1. These results demonstrate that SlyA is a potential target in the development of a novel live attenuated vaccine against S. enterica. SN - 1878-1667 UR - https://www.unboundmedicine.com/medline/citation/21840053/Evaluation_of_Salmonella_enterica_serovar_Typhimurium_and_Choleraesuis_slyA_mutant_strains_for_use_in_live_attenuated_oral_vaccines_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-9571(11)00055-5 DB - PRIME DP - Unbound Medicine ER -