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[Protective effects of puerarin against 1-methyl-4-phenylpyridinium-induced mitochondrial apoptotic death in differentiated SH-SY5Y cells].
Zhongguo Zhong Yao Za Zhi. 2011 May; 36(9):1222-6.ZZ

Abstract

It is well known that puerarin possesses protective activity on neurodegenerative diseases. However, the exact path way involved in the protective effect of puerarin on MPP+ -induced cell death is unclear. In this study, we focused on mitochondria im pairment in the apoptotic process of MPP+ -elicited SH-SY5Y cells and detected the protection of puerarin. As evidenced by Trypan blue assay, the cell viability was significantly decreased by 1 mmol x L(-1) MPP+, but reversed by different concentrations puerarin pre treatment. Flow cytometer analysis revealed that MPP+ -induced SH-SY5Y cells apoptosis and arrested the cells in G2/M phase, where as puerarin pretreatment concentration dependently reversed the apoptosis ratio. In addition to the apoptosis ratio, 50.0 micromol x L(-1) puerarin pretreatment even altered the MPP+ -induced G2/M phase arrest. JC-1 assay suggested that MPP+ significantly opened MMP of the SH-SYSY cells; pretreatment with puerarin attenuated the deterioration of the MMP. Both ELISA and Western blotting showed that puerarin prevented the release of cytochrome c from the mitochondrial interior to the cystol elicited by MPP+. DNA ladder showed that typical DNA ladder was present in the MPP+ -induced SH-SY5Y cells. Additionally, MPP+ enhanced caspase-9 and caspase-3 ac tivity, respectively, while not caspase-8. However,the enhancement was concentration dependently blocked by puerarin pretreatment. Taken together, puerarin can modulate mitochondrial membrane potential and inhibit the cytochrome c releasing-caspase cascade to pre vent MPP+ -induced cell injury.

Authors+Show Affiliations

Jitang College of Hebei United University, Tangshan 063000, China. arthurcyf@163.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

21842654

Citation

Cheng, Yuefa, et al. "[Protective Effects of Puerarin Against 1-methyl-4-phenylpyridinium-induced Mitochondrial Apoptotic Death in Differentiated SH-SY5Y Cells]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 36, no. 9, 2011, pp. 1222-6.
Cheng Y, Zhu G, Guan Y, et al. [Protective effects of puerarin against 1-methyl-4-phenylpyridinium-induced mitochondrial apoptotic death in differentiated SH-SY5Y cells]. Zhongguo Zhong Yao Za Zhi. 2011;36(9):1222-6.
Cheng, Y., Zhu, G., Guan, Y., Liu, Y., Hu, Y., & Li, Q. (2011). [Protective effects of puerarin against 1-methyl-4-phenylpyridinium-induced mitochondrial apoptotic death in differentiated SH-SY5Y cells]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 36(9), 1222-6.
Cheng Y, et al. [Protective Effects of Puerarin Against 1-methyl-4-phenylpyridinium-induced Mitochondrial Apoptotic Death in Differentiated SH-SY5Y Cells]. Zhongguo Zhong Yao Za Zhi. 2011;36(9):1222-6. PubMed PMID: 21842654.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Protective effects of puerarin against 1-methyl-4-phenylpyridinium-induced mitochondrial apoptotic death in differentiated SH-SY5Y cells]. AU - Cheng,Yuefa, AU - Zhu,Guoqi, AU - Guan,Yali, AU - Liu,Yingshuo, AU - Hu,Yan, AU - Li,Qinglin, PY - 2011/8/17/entrez PY - 2011/8/17/pubmed PY - 2011/9/14/medline SP - 1222 EP - 6 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 36 IS - 9 N2 - It is well known that puerarin possesses protective activity on neurodegenerative diseases. However, the exact path way involved in the protective effect of puerarin on MPP+ -induced cell death is unclear. In this study, we focused on mitochondria im pairment in the apoptotic process of MPP+ -elicited SH-SY5Y cells and detected the protection of puerarin. As evidenced by Trypan blue assay, the cell viability was significantly decreased by 1 mmol x L(-1) MPP+, but reversed by different concentrations puerarin pre treatment. Flow cytometer analysis revealed that MPP+ -induced SH-SY5Y cells apoptosis and arrested the cells in G2/M phase, where as puerarin pretreatment concentration dependently reversed the apoptosis ratio. In addition to the apoptosis ratio, 50.0 micromol x L(-1) puerarin pretreatment even altered the MPP+ -induced G2/M phase arrest. JC-1 assay suggested that MPP+ significantly opened MMP of the SH-SYSY cells; pretreatment with puerarin attenuated the deterioration of the MMP. Both ELISA and Western blotting showed that puerarin prevented the release of cytochrome c from the mitochondrial interior to the cystol elicited by MPP+. DNA ladder showed that typical DNA ladder was present in the MPP+ -induced SH-SY5Y cells. Additionally, MPP+ enhanced caspase-9 and caspase-3 ac tivity, respectively, while not caspase-8. However,the enhancement was concentration dependently blocked by puerarin pretreatment. Taken together, puerarin can modulate mitochondrial membrane potential and inhibit the cytochrome c releasing-caspase cascade to pre vent MPP+ -induced cell injury. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/21842654/[Protective_effects_of_puerarin_against_1_methyl_4_phenylpyridinium_induced_mitochondrial_apoptotic_death_in_differentiated_SH_SY5Y_cells]_ L2 - https://antibodies.cancer.gov/detail/CPTC-CASP3-1 DB - PRIME DP - Unbound Medicine ER -