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Nickel nanowires induced and reactive oxygen species mediated apoptosis in human pancreatic adenocarcinoma cells.
Int J Nanomedicine 2011; 6:1475-85IJ

Abstract

BACKGROUND

The ability to evade apoptosis is one of the key properties of cancer. The apoptogenic effect of nickel nanowires (Ni NWs) on cancer cell lines has never been adequately addressed. Due to the unique physicochemical characteristics of Ni NWs, we envision the development of a novel anticancer therapeutics specifically for pancreatic cancer. Thus, we investigated whether Ni NWs induce ROS-mediated apoptosis in human pancreatic adenocarcinoma (Panc-1) cells.

METHODS

In this study Ni NWs were fabricated using the electrodeposition method. Synthesized Ni NWs were physically characterized by energy dispersive X-ray analysis, UV-Vis spectroscopy of NanoDrop 2000 (UV-Vis), magnetization study, scanning electron microscopy, and transmission electron microscopy. Assessment of morphological apoptotic characteristics by phase contrast microscopy (PCM), Ni-NWs-induced apoptosis staining with ethidium bromide (EB) and acridine orange (AO) followed by fluorescence microscopy (FM) was performed. For molecular biological and biochemical characterization, Panc-1 cell culture and cytotoxic effect of Ni NWs were determined by using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. Quantitative apoptosis was analyzed by flow cytometry staining with propidium iodide through cell cycle arrest and generation of ROS using 2', 7'-dichlorofluorescein diacetate fluorescence intensity. In all experiments, Panc-1 cancer cells without any treatment were used as the negative controls.

RESULTS

The intracellular uptake of Ni NWs through endocytosis by Panc-1 cells was observed by PCM. EB and AO staining of FM and MTT assay qualitatively and quantitatively confirmed the extent of apoptosis. Flow cytometric cell cycle arrest and ROS generation indicated Ni NWs as inducers of apoptotic cell death.

CONCLUSION

We investigated the role of Ni NWs as inducers of ROS-mediated apoptosis in Panc-1 cells. These results suggested that Ni NWs could be an effective apoptotic agent for Panc-1 cells and have good potential for further research into a clinical treatment selective for pancreatic cancer.

Authors+Show Affiliations

Applied Biosciences-Bionanotechnology Research, Department of Applied Science, University of Arkansas at Little Rock, Little Rock, Arkansas 72204, USA. mxhossain1@ualr.eduNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21845039

Citation

Hossain, Md Zakir, and Maurice G. Kleve. "Nickel Nanowires Induced and Reactive Oxygen Species Mediated Apoptosis in Human Pancreatic Adenocarcinoma Cells." International Journal of Nanomedicine, vol. 6, 2011, pp. 1475-85.
Hossain MZ, Kleve MG. Nickel nanowires induced and reactive oxygen species mediated apoptosis in human pancreatic adenocarcinoma cells. Int J Nanomedicine. 2011;6:1475-85.
Hossain, M. Z., & Kleve, M. G. (2011). Nickel nanowires induced and reactive oxygen species mediated apoptosis in human pancreatic adenocarcinoma cells. International Journal of Nanomedicine, 6, pp. 1475-85. doi:10.2147/IJN.S21697.
Hossain MZ, Kleve MG. Nickel Nanowires Induced and Reactive Oxygen Species Mediated Apoptosis in Human Pancreatic Adenocarcinoma Cells. Int J Nanomedicine. 2011;6:1475-85. PubMed PMID: 21845039.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nickel nanowires induced and reactive oxygen species mediated apoptosis in human pancreatic adenocarcinoma cells. AU - Hossain,Md Zakir, AU - Kleve,Maurice G, Y1 - 2011/07/13/ PY - 2011/8/17/entrez PY - 2011/8/17/pubmed PY - 2011/12/13/medline KW - MTT assay KW - Ni NWs KW - Panc-1 KW - ROS KW - apoptosis KW - cell cycle arrest KW - flow cytometry SP - 1475 EP - 85 JF - International journal of nanomedicine JO - Int J Nanomedicine VL - 6 N2 - BACKGROUND: The ability to evade apoptosis is one of the key properties of cancer. The apoptogenic effect of nickel nanowires (Ni NWs) on cancer cell lines has never been adequately addressed. Due to the unique physicochemical characteristics of Ni NWs, we envision the development of a novel anticancer therapeutics specifically for pancreatic cancer. Thus, we investigated whether Ni NWs induce ROS-mediated apoptosis in human pancreatic adenocarcinoma (Panc-1) cells. METHODS: In this study Ni NWs were fabricated using the electrodeposition method. Synthesized Ni NWs were physically characterized by energy dispersive X-ray analysis, UV-Vis spectroscopy of NanoDrop 2000 (UV-Vis), magnetization study, scanning electron microscopy, and transmission electron microscopy. Assessment of morphological apoptotic characteristics by phase contrast microscopy (PCM), Ni-NWs-induced apoptosis staining with ethidium bromide (EB) and acridine orange (AO) followed by fluorescence microscopy (FM) was performed. For molecular biological and biochemical characterization, Panc-1 cell culture and cytotoxic effect of Ni NWs were determined by using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. Quantitative apoptosis was analyzed by flow cytometry staining with propidium iodide through cell cycle arrest and generation of ROS using 2', 7'-dichlorofluorescein diacetate fluorescence intensity. In all experiments, Panc-1 cancer cells without any treatment were used as the negative controls. RESULTS: The intracellular uptake of Ni NWs through endocytosis by Panc-1 cells was observed by PCM. EB and AO staining of FM and MTT assay qualitatively and quantitatively confirmed the extent of apoptosis. Flow cytometric cell cycle arrest and ROS generation indicated Ni NWs as inducers of apoptotic cell death. CONCLUSION: We investigated the role of Ni NWs as inducers of ROS-mediated apoptosis in Panc-1 cells. These results suggested that Ni NWs could be an effective apoptotic agent for Panc-1 cells and have good potential for further research into a clinical treatment selective for pancreatic cancer. SN - 1178-2013 UR - https://www.unboundmedicine.com/medline/citation/21845039/Nickel_nanowires_induced_and_reactive_oxygen_species_mediated_apoptosis_in_human_pancreatic_adenocarcinoma_cells_ L2 - https://dx.doi.org/10.2147/IJN.S21697 DB - PRIME DP - Unbound Medicine ER -