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Inhibition of LPS-induced inflammatory biomarkers by ethyl acetate fraction of Patrinia scabiosaefolia through suppression of NF-κB activation in RAW 264.7 cells.
Immunopharmacol Immunotoxicol. 2012 Apr; 34(2):282-91.II

Abstract

Patrinia scabiosaefolia (PS) has been used for curing various types of inflammatory-related disorders. However, the precise mechanism of the anti-inflammatory activity of PS remains unclear. Here, we investigated the anti-inflammatory effects of several fractions isolated from the PS in RAW 264.7 macrophages. The results indicated that the ethyl acetate fraction of PS (EAPS) concentration highly suppressed lipopolysaccharide (LPS)-induced nitric oxide (NO) and IL-6 productions without a cytotoxic effect on RAW 264.7 cells. EAPS inhibited the expressions of LPS-induced iNOS and COX-2 protein and their mRNA in a dose-dependent manner. Particularly, EAPS suppressed the level of nuclear factor-κB (NF-κB) activity, which was linked with the suppression of LPS-induced phosphorylation of p65 at serine 276 and p65 translocation into nuclei, but not MAPK signaling. In addition, treatment with EAPS inhibited the production of TNF-α in LPS-injected mice and suppressed the production of IL-6 and TNF-α in LPS-stimulated splenocytes from BALB/c mice. Therefore, we demonstrate here that Patrinia scabiosaefolia potentially inhibits the biomarkers related to inflammation through the blocking of NF-κB p65 activation, and it may be a potential therapeutic candidate for the treatment of inflammatory diseases.

Authors+Show Affiliations

College of Oriental Medicine and Institute of Oriental Medicine, Kyung Hee University, Hoegidong Dongdaemungu, Seoul, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21854107

Citation

Lee, Eun-Jung, et al. "Inhibition of LPS-induced Inflammatory Biomarkers By Ethyl Acetate Fraction of Patrinia Scabiosaefolia Through Suppression of NF-κB Activation in RAW 264.7 Cells." Immunopharmacology and Immunotoxicology, vol. 34, no. 2, 2012, pp. 282-91.
Lee EJ, Kim C, Kim JY, et al. Inhibition of LPS-induced inflammatory biomarkers by ethyl acetate fraction of Patrinia scabiosaefolia through suppression of NF-κB activation in RAW 264.7 cells. Immunopharmacol Immunotoxicol. 2012;34(2):282-91.
Lee, E. J., Kim, C., Kim, J. Y., Kim, S. M., Nam, D., Jang, H. J., Kim, S. H., Shim, B. S., Ahn, K. S., Choi, S. H., Jung, S. H., & Ahn, K. S. (2012). Inhibition of LPS-induced inflammatory biomarkers by ethyl acetate fraction of Patrinia scabiosaefolia through suppression of NF-κB activation in RAW 264.7 cells. Immunopharmacology and Immunotoxicology, 34(2), 282-91. https://doi.org/10.3109/08923973.2011.602412
Lee EJ, et al. Inhibition of LPS-induced Inflammatory Biomarkers By Ethyl Acetate Fraction of Patrinia Scabiosaefolia Through Suppression of NF-κB Activation in RAW 264.7 Cells. Immunopharmacol Immunotoxicol. 2012;34(2):282-91. PubMed PMID: 21854107.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of LPS-induced inflammatory biomarkers by ethyl acetate fraction of Patrinia scabiosaefolia through suppression of NF-κB activation in RAW 264.7 cells. AU - Lee,Eun-Jung, AU - Kim,Chulwon, AU - Kim,Jin-Young, AU - Kim,Sung-Moo, AU - Nam,Dongwoo, AU - Jang,Hyeung-Jin, AU - Kim,Sung-Hoon, AU - Shim,Bum Sang, AU - Ahn,Kyoo Seok, AU - Choi,Seung-Hoon, AU - Jung,Sang Hoon, AU - Ahn,Kwang Seok, Y1 - 2011/08/19/ PY - 2011/8/23/entrez PY - 2011/8/23/pubmed PY - 2012/7/13/medline SP - 282 EP - 91 JF - Immunopharmacology and immunotoxicology JO - Immunopharmacol Immunotoxicol VL - 34 IS - 2 N2 - Patrinia scabiosaefolia (PS) has been used for curing various types of inflammatory-related disorders. However, the precise mechanism of the anti-inflammatory activity of PS remains unclear. Here, we investigated the anti-inflammatory effects of several fractions isolated from the PS in RAW 264.7 macrophages. The results indicated that the ethyl acetate fraction of PS (EAPS) concentration highly suppressed lipopolysaccharide (LPS)-induced nitric oxide (NO) and IL-6 productions without a cytotoxic effect on RAW 264.7 cells. EAPS inhibited the expressions of LPS-induced iNOS and COX-2 protein and their mRNA in a dose-dependent manner. Particularly, EAPS suppressed the level of nuclear factor-κB (NF-κB) activity, which was linked with the suppression of LPS-induced phosphorylation of p65 at serine 276 and p65 translocation into nuclei, but not MAPK signaling. In addition, treatment with EAPS inhibited the production of TNF-α in LPS-injected mice and suppressed the production of IL-6 and TNF-α in LPS-stimulated splenocytes from BALB/c mice. Therefore, we demonstrate here that Patrinia scabiosaefolia potentially inhibits the biomarkers related to inflammation through the blocking of NF-κB p65 activation, and it may be a potential therapeutic candidate for the treatment of inflammatory diseases. SN - 1532-2513 UR - https://www.unboundmedicine.com/medline/citation/21854107/Inhibition_of_LPS_induced_inflammatory_biomarkers_by_ethyl_acetate_fraction_of_Patrinia_scabiosaefolia_through_suppression_of_NF_κB_activation_in_RAW_264_7_cells_ L2 - https://www.tandfonline.com/doi/full/10.3109/08923973.2011.602412 DB - PRIME DP - Unbound Medicine ER -