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Protective effects of muscone on ischemia-reperfusion injury in cardiac myocytes.
J Ethnopharmacol. 2011 Oct 31; 138(1):34-9.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Musk has been traditionally used in Chinese medicine as the main ingredient of many formulations for the treatment of chest pain and angina pectoris.

AIM OF THE STUDY

To investigate the protective effects of muscone (the active ingredient of musk) on ischemia-reperfusion (I/R) injury induced by hypoxia and low glucose in primary cultured rat cardiac myocytes.

MATERIALS AND METHODS

Primary cultures of neonatal rat cardiac myocytes were subjected to ischemia-reperfusion in media, with or without muscone. Cell viability, release of lactic acid dehydrogenase (LDH), superoxide dismutase (SOD) activity, malondialdehyde (MDA) levels, creatine kinase (CK) and caspase-3 activities, as well as intracellular free Ca(2+) concentrations, were measured. Cellular apoptosis and mitochondrial membrane potential (MMP) were assessed by flow cytometry, and the expression of Bcl-2 and Bax proteins was assessed by Western blotting.

RESULTS

Following the exposure of cardiac myocytes to ischemia-reperfusion, there was a marked decrease in pulsating frequency, cell viability, SOD activity, MMP, and the expression of Bcl-2 protein, accompanied by increased LDH release, MDA production, CK and caspase-3 activities, intracellular free Ca(2+) concentrations, rate of apoptosis, and expression of Bax protein. Pretreatment with muscone (0.215, 0.43, 0.86 μg/mL) prior to I/R injury significantly attenuated the above changes.

CONCLUSION

Muscone has a protective effect against I/R injury in cardiac myocytes, indicating that muscone may potentially provide therapeutic benefit in I/R injury by inhibiting cellular oxidative stress and apoptosis.

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Authors+Show Affiliations

Wu Q
Faculty of Chinese Medicine, Macau University of Science and Technology, Macau 999078, China. qbwu@must.edu.mo
Li H
No affiliation info available
Wu Y
No affiliation info available
Shen W
No affiliation info available
Zeng L
No affiliation info available
Cheng H
No affiliation info available
He L
No affiliation info available

MeSH

AnimalsAntioxidantsApoptosisArtiodactylaCalciumCaspase 3Cell SurvivalCells, CulturedCreatine KinaseCycloparaffinsL-Lactate DehydrogenaseMalondialdehydeMembrane Potential, MitochondrialMyocardial Reperfusion InjuryMyocardiumMyocytes, CardiacOxidative StressProto-Oncogene Proteins c-bcl-2RatsRats, WistarSuperoxide Dismutasebcl-2-Associated X Protein

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21856397

Citation

Wu, Qibiao, et al. "Protective Effects of Muscone On Ischemia-reperfusion Injury in Cardiac Myocytes." Journal of Ethnopharmacology, vol. 138, no. 1, 2011, pp. 34-9.
Wu Q, Li H, Wu Y, et al. Protective effects of muscone on ischemia-reperfusion injury in cardiac myocytes. J Ethnopharmacol. 2011;138(1):34-9.
Wu, Q., Li, H., Wu, Y., Shen, W., Zeng, L., Cheng, H., & He, L. (2011). Protective effects of muscone on ischemia-reperfusion injury in cardiac myocytes. Journal of Ethnopharmacology, 138(1), 34-9. https://doi.org/10.1016/j.jep.2011.08.009
Wu Q, et al. Protective Effects of Muscone On Ischemia-reperfusion Injury in Cardiac Myocytes. J Ethnopharmacol. 2011 Oct 31;138(1):34-9. PubMed PMID: 21856397.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of muscone on ischemia-reperfusion injury in cardiac myocytes. AU - Wu,Qibiao, AU - Li,Haitao, AU - Wu,Ye, AU - Shen,Weixing, AU - Zeng,Li, AU - Cheng,Haibo, AU - He,Ling, Y1 - 2011/08/11/ PY - 2011/04/21/received PY - 2011/07/30/revised PY - 2011/08/02/accepted PY - 2011/8/23/entrez PY - 2011/8/23/pubmed PY - 2012/5/4/medline SP - 34 EP - 9 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 138 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Musk has been traditionally used in Chinese medicine as the main ingredient of many formulations for the treatment of chest pain and angina pectoris. AIM OF THE STUDY: To investigate the protective effects of muscone (the active ingredient of musk) on ischemia-reperfusion (I/R) injury induced by hypoxia and low glucose in primary cultured rat cardiac myocytes. MATERIALS AND METHODS: Primary cultures of neonatal rat cardiac myocytes were subjected to ischemia-reperfusion in media, with or without muscone. Cell viability, release of lactic acid dehydrogenase (LDH), superoxide dismutase (SOD) activity, malondialdehyde (MDA) levels, creatine kinase (CK) and caspase-3 activities, as well as intracellular free Ca(2+) concentrations, were measured. Cellular apoptosis and mitochondrial membrane potential (MMP) were assessed by flow cytometry, and the expression of Bcl-2 and Bax proteins was assessed by Western blotting. RESULTS: Following the exposure of cardiac myocytes to ischemia-reperfusion, there was a marked decrease in pulsating frequency, cell viability, SOD activity, MMP, and the expression of Bcl-2 protein, accompanied by increased LDH release, MDA production, CK and caspase-3 activities, intracellular free Ca(2+) concentrations, rate of apoptosis, and expression of Bax protein. Pretreatment with muscone (0.215, 0.43, 0.86 μg/mL) prior to I/R injury significantly attenuated the above changes. CONCLUSION: Muscone has a protective effect against I/R injury in cardiac myocytes, indicating that muscone may potentially provide therapeutic benefit in I/R injury by inhibiting cellular oxidative stress and apoptosis. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/21856397/Protective_effects_of_muscone_on_ischemia_reperfusion_injury_in_cardiac_myocytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(11)00582-4 DB - PRIME DP - Unbound Medicine ER -