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Novel surface molecularly imprinted sol-gel polymer applied to the online solid phase extraction of methyl-3-quinoxaline-2-carboxylic acid and quinoxaline-2-carboxylic acid from pork muscle.
Anal Bioanal Chem. 2011 Oct; 401(7):2291-9.AB

Abstract

A new molecularly imprinted polymer (MIP), selective for major metabolites of quinoxaline-1,4-dioxides was firstly prepared by combining surface molecular imprinting technique with the sol-gel process. Methyl-3-quinoxaline-2-carboxylic acid (MQCA) was used as template, 3-aminopropyltriethoxysilane as functional monomer, and tetraethoxysilicane as cross-linker. The MIP was characterized by Fourier transform infrared and evaluated through static adsorption experiments. The results indicated that MIP had high adsorption capacity, fast binding kinetics for MQCA, and the polymer showed a high degree of cross-reactivity for quinoxaline-2-carboxylic acid (QCA). The MIP was then applied as a selective sorbent in an online solid phase extraction (SPE) coupled with high-performance liquid chromatography (HPLC). For a 50-mL sample solution, enrichment factors of 1,349 and 1,046 for QCA and MQCA, respectively, and limits of detection (S/N=3) of 0.8 and 2 ng L(-1) for QCA and MQCA, respectively, were obtained (corresponding to 0.02 and 0.04 ng g(-1) in solid samples for final 100 mL of sample solutions of 5 g of pork). The sample preparation protocol was simplified and only included one step extraction with acetonitrile (MeCN) after the release of target analytes through acidic hydrolysis without further sample cleanup. The new MIP-SPE-HPLC method was successfully applied to the quantification of trace QCA and MQCA in pork muscle with good recoveries ranging from 67% to 80% and RSD below 8%.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Duan ZJ
Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, China.
Fan LP
No affiliation info available
Fang GZ
No affiliation info available
Yi JH
No affiliation info available
Wang S
No affiliation info available

MeSH

AdsorptionAnimalsChromatography, High Pressure LiquidMeatMolecular ImprintingMusclesPolymersQuinoxalinesSilica GelSolid Phase ExtractionSwine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21858646

Citation

Duan, Zhen-Juan, et al. "Novel Surface Molecularly Imprinted Sol-gel Polymer Applied to the Online Solid Phase Extraction of Methyl-3-quinoxaline-2-carboxylic Acid and Quinoxaline-2-carboxylic Acid From Pork Muscle." Analytical and Bioanalytical Chemistry, vol. 401, no. 7, 2011, pp. 2291-9.
Duan ZJ, Fan LP, Fang GZ, et al. Novel surface molecularly imprinted sol-gel polymer applied to the online solid phase extraction of methyl-3-quinoxaline-2-carboxylic acid and quinoxaline-2-carboxylic acid from pork muscle. Anal Bioanal Chem. 2011;401(7):2291-9.
Duan, Z. J., Fan, L. P., Fang, G. Z., Yi, J. H., & Wang, S. (2011). Novel surface molecularly imprinted sol-gel polymer applied to the online solid phase extraction of methyl-3-quinoxaline-2-carboxylic acid and quinoxaline-2-carboxylic acid from pork muscle. Analytical and Bioanalytical Chemistry, 401(7), 2291-9. https://doi.org/10.1007/s00216-011-5329-0
Duan ZJ, et al. Novel Surface Molecularly Imprinted Sol-gel Polymer Applied to the Online Solid Phase Extraction of Methyl-3-quinoxaline-2-carboxylic Acid and Quinoxaline-2-carboxylic Acid From Pork Muscle. Anal Bioanal Chem. 2011;401(7):2291-9. PubMed PMID: 21858646.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel surface molecularly imprinted sol-gel polymer applied to the online solid phase extraction of methyl-3-quinoxaline-2-carboxylic acid and quinoxaline-2-carboxylic acid from pork muscle. AU - Duan,Zhen-Juan, AU - Fan,Li-Peng, AU - Fang,Guo-Zhen, AU - Yi,Jiang-Hua, AU - Wang,Shuo, Y1 - 2011/08/21/ PY - 2011/07/01/received PY - 2011/08/06/accepted PY - 2011/08/03/revised PY - 2011/8/23/entrez PY - 2011/8/23/pubmed PY - 2012/1/13/medline SP - 2291 EP - 9 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 401 IS - 7 N2 - A new molecularly imprinted polymer (MIP), selective for major metabolites of quinoxaline-1,4-dioxides was firstly prepared by combining surface molecular imprinting technique with the sol-gel process. Methyl-3-quinoxaline-2-carboxylic acid (MQCA) was used as template, 3-aminopropyltriethoxysilane as functional monomer, and tetraethoxysilicane as cross-linker. The MIP was characterized by Fourier transform infrared and evaluated through static adsorption experiments. The results indicated that MIP had high adsorption capacity, fast binding kinetics for MQCA, and the polymer showed a high degree of cross-reactivity for quinoxaline-2-carboxylic acid (QCA). The MIP was then applied as a selective sorbent in an online solid phase extraction (SPE) coupled with high-performance liquid chromatography (HPLC). For a 50-mL sample solution, enrichment factors of 1,349 and 1,046 for QCA and MQCA, respectively, and limits of detection (S/N=3) of 0.8 and 2 ng L(-1) for QCA and MQCA, respectively, were obtained (corresponding to 0.02 and 0.04 ng g(-1) in solid samples for final 100 mL of sample solutions of 5 g of pork). The sample preparation protocol was simplified and only included one step extraction with acetonitrile (MeCN) after the release of target analytes through acidic hydrolysis without further sample cleanup. The new MIP-SPE-HPLC method was successfully applied to the quantification of trace QCA and MQCA in pork muscle with good recoveries ranging from 67% to 80% and RSD below 8%. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/21858646/Novel_surface_molecularly_imprinted_sol_gel_polymer_applied_to_the_online_solid_phase_extraction_of_methyl_3_quinoxaline_2_carboxylic_acid_and_quinoxaline_2_carboxylic_acid_from_pork_muscle_ L2 - https://dx.doi.org/10.1007/s00216-011-5329-0 DB - PRIME DP - Unbound Medicine ER -
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