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[High-frequency electro-acupuncture stimulation modulates intracerebral γ-aminobutyric acid content in rat model of Parkinson's disease].

Abstract

The purpose of the present study is to observe the effect of electro-acupuncture (EA) stimulation on intracerebral neurotransmitters in a rat model of Parkinson's disease (PD), and explore the possible mechanism. We used 6-hydroxydopamine (6-OHDA) injection in medial forebrain bundle (MFB) in the right brain of Sprague Dawley (SD) rat to establish the parkinsonian rat model, and randomly divided the PD rats into model and 100 Hz EA stimulation groups (n =10 in each group). EA stimulation group received 4 courses of EA stimulation on Baihui (GV-20) and Dazhui (GV-14) acupuncture points. Moreover, ten rats were randomly selected as sham operation group, only receiving normal saline (NS) injection in MFB. Then apomorphine (APO)-induced rotational behavior in different groups was recorded, and the contents of γ-aminobutyric acid (GABA) in the brain were analyzed with high pressure/performance liquid chromatography-electrochemical detection (HPLC-ECD). The results showed that model group exhibited abnormal rotational behavior with APO treatment, suggesting the successful establishment of PD model. Compared with sham operation group, model group showed increased GABA contents in cortex and striatum, as well as decreased GABA content in ventral midbrain, on the lesioned side. EA stimulation could effectively ameliorate the abnormal rotational behavior of PD rat. Compared with the model group, EA stimulation decreased the ratio of GABA content on the lesioned side to that on unlesioned side in the cortex, while increased the ratios in the striatum and cerebellum. However, there was no difference of the ratio in the ventral midbrain among three groups. These results suggest high-frequency EA stimulation significantly improves the abnormal behavior of PD rats, which may exert through enhancing the inhibitory effect of cerebellum-basal ganglia-cortical loop on motor center.

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  • Authors+Show Affiliations

    ,

    Department of Physiology, Capital Medical University, Beijing 100069, China.

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    MeSH

    Acupuncture Therapy
    Animals
    Brain
    Electroacupuncture
    Male
    Motor Cortex
    Oxidopamine
    Parkinson Disease, Secondary
    Rats
    Rats, Sprague-Dawley
    gamma-Aminobutyric Acid

    Pub Type(s)

    English Abstract
    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    chi

    PubMed ID

    21861048

    Citation

    Du, Jing, et al. "[High-frequency Electro-acupuncture Stimulation Modulates Intracerebral Γ-aminobutyric Acid Content in Rat Model of Parkinson's Disease]." Sheng Li Xue Bao : [Acta Physiologica Sinica], vol. 63, no. 4, 2011, pp. 305-10.
    Du J, Sun ZL, Jia J, et al. [High-frequency electro-acupuncture stimulation modulates intracerebral γ-aminobutyric acid content in rat model of Parkinson's disease]. Sheng Li Xue Bao. 2011;63(4):305-10.
    Du, J., Sun, Z. L., Jia, J., Wang, X., & Wang, X. M. (2011). [High-frequency electro-acupuncture stimulation modulates intracerebral γ-aminobutyric acid content in rat model of Parkinson's disease]. Sheng Li Xue Bao : [Acta Physiologica Sinica], 63(4), pp. 305-10.
    Du J, et al. [High-frequency Electro-acupuncture Stimulation Modulates Intracerebral Γ-aminobutyric Acid Content in Rat Model of Parkinson's Disease]. Sheng Li Xue Bao. 2011 Aug 25;63(4):305-10. PubMed PMID: 21861048.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - [High-frequency electro-acupuncture stimulation modulates intracerebral γ-aminobutyric acid content in rat model of Parkinson's disease]. AU - Du,Jing, AU - Sun,Zuo-Li, AU - Jia,Jun, AU - Wang,Xuan, AU - Wang,Xiao-Min, PY - 2011/8/24/entrez PY - 2011/8/24/pubmed PY - 2013/2/22/medline SP - 305 EP - 10 JF - Sheng li xue bao : [Acta physiologica Sinica] JO - Sheng Li Xue Bao VL - 63 IS - 4 N2 - The purpose of the present study is to observe the effect of electro-acupuncture (EA) stimulation on intracerebral neurotransmitters in a rat model of Parkinson's disease (PD), and explore the possible mechanism. We used 6-hydroxydopamine (6-OHDA) injection in medial forebrain bundle (MFB) in the right brain of Sprague Dawley (SD) rat to establish the parkinsonian rat model, and randomly divided the PD rats into model and 100 Hz EA stimulation groups (n =10 in each group). EA stimulation group received 4 courses of EA stimulation on Baihui (GV-20) and Dazhui (GV-14) acupuncture points. Moreover, ten rats were randomly selected as sham operation group, only receiving normal saline (NS) injection in MFB. Then apomorphine (APO)-induced rotational behavior in different groups was recorded, and the contents of γ-aminobutyric acid (GABA) in the brain were analyzed with high pressure/performance liquid chromatography-electrochemical detection (HPLC-ECD). The results showed that model group exhibited abnormal rotational behavior with APO treatment, suggesting the successful establishment of PD model. Compared with sham operation group, model group showed increased GABA contents in cortex and striatum, as well as decreased GABA content in ventral midbrain, on the lesioned side. EA stimulation could effectively ameliorate the abnormal rotational behavior of PD rat. Compared with the model group, EA stimulation decreased the ratio of GABA content on the lesioned side to that on unlesioned side in the cortex, while increased the ratios in the striatum and cerebellum. However, there was no difference of the ratio in the ventral midbrain among three groups. These results suggest high-frequency EA stimulation significantly improves the abnormal behavior of PD rats, which may exert through enhancing the inhibitory effect of cerebellum-basal ganglia-cortical loop on motor center. SN - 0371-0874 UR - https://www.unboundmedicine.com/medline/citation/21861048/ L2 - http://www.actaps.com.cn/qikan/manage/wenzhang/2011-4-03.pdf DB - PRIME DP - Unbound Medicine ER -