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The tunica adventitia of human arteries and veins as a source of mesenchymal stem cells.
Stem Cells Dev 2012; 21(8):1299-308SC

Abstract

We previously demonstrated that human pericytes, which encircle capillaries and microvessels, give rise in culture to genuine mesenchymal stem cells (MSCs). This raised the question as to whether all MSC are derived from pericytes. Pericytes and other cells defined on differential expression of CD34, CD31, and CD146 were sorted from the stromal vascular fraction of human white adipose tissue. Besides pericytes, CD34+ CD31- CD146- CD45- cells, which reside in the outmost layer of blood vessels, the tunica adventitia, natively expressed MSC markers and gave rise in culture to clonogenic multipotent progenitors identical to standard bone marrow-derived MSC. Despite common MSC features and developmental properties, adventitial cells and pericytes retain distinct phenotypes and genotypes through culture. However, in the presence of growth factors involved in vascular remodeling, adventitial cells acquire a pericytes-like phenotype. In conclusion, we demonstrate the co-existence of 2 separate perivascular MSC progenitors: pericytes in capillaries and microvessels and adventitial cells around larger vessels.

Authors+Show Affiliations

Department of Orthopaedic Surgery, Orthopaedic Hospital Research Center, UCLA and Orthopaedic Hospital, Los Angeles, California 90095, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21861688

Citation

Corselli, Mirko, et al. "The Tunica Adventitia of Human Arteries and Veins as a Source of Mesenchymal Stem Cells." Stem Cells and Development, vol. 21, no. 8, 2012, pp. 1299-308.
Corselli M, Chen CW, Sun B, et al. The tunica adventitia of human arteries and veins as a source of mesenchymal stem cells. Stem Cells Dev. 2012;21(8):1299-308.
Corselli, M., Chen, C. W., Sun, B., Yap, S., Rubin, J. P., & Péault, B. (2012). The tunica adventitia of human arteries and veins as a source of mesenchymal stem cells. Stem Cells and Development, 21(8), pp. 1299-308. doi:10.1089/scd.2011.0200.
Corselli M, et al. The Tunica Adventitia of Human Arteries and Veins as a Source of Mesenchymal Stem Cells. Stem Cells Dev. 2012 May 20;21(8):1299-308. PubMed PMID: 21861688.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The tunica adventitia of human arteries and veins as a source of mesenchymal stem cells. AU - Corselli,Mirko, AU - Chen,Chien-Wen, AU - Sun,Bin, AU - Yap,Solomon, AU - Rubin,J Peter, AU - Péault,Bruno, Y1 - 2011/10/18/ PY - 2011/8/25/entrez PY - 2011/8/25/pubmed PY - 2012/8/30/medline SP - 1299 EP - 308 JF - Stem cells and development JO - Stem Cells Dev. VL - 21 IS - 8 N2 - We previously demonstrated that human pericytes, which encircle capillaries and microvessels, give rise in culture to genuine mesenchymal stem cells (MSCs). This raised the question as to whether all MSC are derived from pericytes. Pericytes and other cells defined on differential expression of CD34, CD31, and CD146 were sorted from the stromal vascular fraction of human white adipose tissue. Besides pericytes, CD34+ CD31- CD146- CD45- cells, which reside in the outmost layer of blood vessels, the tunica adventitia, natively expressed MSC markers and gave rise in culture to clonogenic multipotent progenitors identical to standard bone marrow-derived MSC. Despite common MSC features and developmental properties, adventitial cells and pericytes retain distinct phenotypes and genotypes through culture. However, in the presence of growth factors involved in vascular remodeling, adventitial cells acquire a pericytes-like phenotype. In conclusion, we demonstrate the co-existence of 2 separate perivascular MSC progenitors: pericytes in capillaries and microvessels and adventitial cells around larger vessels. SN - 1557-8534 UR - https://www.unboundmedicine.com/medline/citation/21861688/The_tunica_adventitia_of_human_arteries_and_veins_as_a_source_of_mesenchymal_stem_cells_ L2 - https://www.liebertpub.com/doi/full/10.1089/scd.2011.0200?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -