Tags

Type your tag names separated by a space and hit enter

Evaluation of different micro/nanobeads used as amplifiers in QCM immunosensor for more sensitive detection of E. coli O157:H7.
Biosens Bioelectron. 2011 Nov 15; 29(1):23-8.BB

Abstract

Micro/nanobeads with different materials (magnetic, silica and polymer) and different sizes (diameters from 30nm to 970nm) were investigated for their use as amplifiers in a quartz crystal microbalance (QCM) immunosensor for more sensitive detection of Escherichia coli O157:H7. The micro/nanobeads were conjugated with anti-E. coli antibodies. E. coli O157:H7 cells were first captured by the first antibody immobilized on the electrode surface, and then micro/nanobeads labeled secondary antibodies attached to the cells, and finally the complexes of antibody-E. coli-antibody modified beads were formed. The results showed that antibody-labeled beads lead to signal amplification in both the change in frequency (ΔF) and the change in resistance (ΔR). Since the penetration depth of the oscillation-induced shear-waves for a ∼8MHz crystal is limited to 200nm, the interpretation of how the signal is amplified by the adsorbed particles was represented in terms of the coupled-oscillator theory. The amplification is not sensed in terms of increase in mass on the sensor surface. Amplification is sensed as a change in bacterial resonance frequency when the spheres adsorb to the bacteria. The change in the values of ΔF caused by different micro/nanobeads (amplifiers) attaching on target bacterial cells is indicative of the ratio between the resonance frequency of the absorbed bacterial-particle complex (ω(s)), and the resonance frequency of the crystal (ω).

Links

Publisher Full Text

Authors+Show Affiliations

Jiang X
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China.
Wang R
No affiliation info available
Wang Y
No affiliation info available
Su X
No affiliation info available
Ying Y
No affiliation info available
Wang J
No affiliation info available
Li Y
No affiliation info available

MeSH

Antibodies, BacterialBacteriological TechniquesBiosensing TechniquesEscherichia coli O157HumansMicroscopy, Electron, ScanningNanoconjugatesParticle SizeQuartz Crystal Microbalance Techniques

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21862307

Citation

Jiang, Xuesong, et al. "Evaluation of Different Micro/nanobeads Used as Amplifiers in QCM Immunosensor for More Sensitive Detection of E. Coli O157:H7." Biosensors & Bioelectronics, vol. 29, no. 1, 2011, pp. 23-8.
Jiang X, Wang R, Wang Y, et al. Evaluation of different micro/nanobeads used as amplifiers in QCM immunosensor for more sensitive detection of E. coli O157:H7. Biosens Bioelectron. 2011;29(1):23-8.
Jiang, X., Wang, R., Wang, Y., Su, X., Ying, Y., Wang, J., & Li, Y. (2011). Evaluation of different micro/nanobeads used as amplifiers in QCM immunosensor for more sensitive detection of E. coli O157:H7. Biosensors & Bioelectronics, 29(1), 23-8. https://doi.org/10.1016/j.bios.2011.07.059
Jiang X, et al. Evaluation of Different Micro/nanobeads Used as Amplifiers in QCM Immunosensor for More Sensitive Detection of E. Coli O157:H7. Biosens Bioelectron. 2011 Nov 15;29(1):23-8. PubMed PMID: 21862307.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of different micro/nanobeads used as amplifiers in QCM immunosensor for more sensitive detection of E. coli O157:H7. AU - Jiang,Xuesong, AU - Wang,Ronghui, AU - Wang,Yun, AU - Su,Xiaoli, AU - Ying,Yibin, AU - Wang,Jianping, AU - Li,Yanbin, Y1 - 2011/07/30/ PY - 2011/03/05/received PY - 2011/07/01/revised PY - 2011/07/04/accepted PY - 2011/8/25/entrez PY - 2011/8/25/pubmed PY - 2012/2/4/medline SP - 23 EP - 8 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 29 IS - 1 N2 - Micro/nanobeads with different materials (magnetic, silica and polymer) and different sizes (diameters from 30nm to 970nm) were investigated for their use as amplifiers in a quartz crystal microbalance (QCM) immunosensor for more sensitive detection of Escherichia coli O157:H7. The micro/nanobeads were conjugated with anti-E. coli antibodies. E. coli O157:H7 cells were first captured by the first antibody immobilized on the electrode surface, and then micro/nanobeads labeled secondary antibodies attached to the cells, and finally the complexes of antibody-E. coli-antibody modified beads were formed. The results showed that antibody-labeled beads lead to signal amplification in both the change in frequency (ΔF) and the change in resistance (ΔR). Since the penetration depth of the oscillation-induced shear-waves for a ∼8MHz crystal is limited to 200nm, the interpretation of how the signal is amplified by the adsorbed particles was represented in terms of the coupled-oscillator theory. The amplification is not sensed in terms of increase in mass on the sensor surface. Amplification is sensed as a change in bacterial resonance frequency when the spheres adsorb to the bacteria. The change in the values of ΔF caused by different micro/nanobeads (amplifiers) attaching on target bacterial cells is indicative of the ratio between the resonance frequency of the absorbed bacterial-particle complex (ω(s)), and the resonance frequency of the crystal (ω). SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/21862307/Evaluation_of_different_micro/nanobeads_used_as_amplifiers_in_QCM_immunosensor_for_more_sensitive_detection_of_E__coli_O157:H7_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(11)00507-0 DB - PRIME DP - Unbound Medicine ER -