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Effects of low environmental salinity on the cellular profiles and expression of Na+, K+-ATPase and Na+, K+, 2Cl- cotransporter 1 of branchial mitochondrion-rich cells in the juvenile marine fish Monodactylus argenteus.
Fish Physiol Biochem. 2012 Jun; 38(3):665-78.FP

Abstract

The goal of this study was to determine the osmoregulatory ability of a juvenile marine fish, silver moony (Monodactylus argenteus), for the purpose of developing a new experimental species for ecophysiological research. In this study, M. argenteus was acclimated to freshwater (FW), brackish water (BW), or seawater (SW). The salinity tolerance of this euryhaline species was effective, and the fish survived well upon osmotic challenges. The largest apical surface of mitochondrion-rich cells was found in the FW individuals. Immunohistochemical staining revealed that Na(+), K(+)-ATPase immunoreactive (NKA-IR) cells were distributed in the interlamellar region of the gill filaments of the silver moony in all experimental groups. In addition to the filaments, NKA-IR cells were also found in the lamellae of the FW individuals. The number of NKA-IR cells in the gills of the FW individuals exceeded that of the BW and SW individuals. The NKA-IR cells of FW and SW individuals exhibited bigger size than that of BW fish. The NKA activities and protein expression of the NKA α-subunit in the gills of the FW individuals were significantly higher than in the BW and SW groups. Additionally, the relative amounts of Na(+), K(+), 2Cl(-) cotransporter 1 (NKCC1) were salinity-dependent in the gills. Immunofluorescent signals of NKCC1 were localized to the basolateral membrane of NKA-IR cells in all groups. In the gills of the FW individuals, however, some NKA-IR cells did not exhibit a basolateral NKCC1 signal. In conclusion, the present study illustrated the osmoregulatory mechanisms of this easy- and economic-to-rear marine teleost with euryhaline capacity and proved the silver moony to be a good experimental animal.

Authors+Show Affiliations

Department of Life Sciences, National Chung Hsing University, Taichung 402, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21863333

Citation

Kang, Chao-Kai, et al. "Effects of Low Environmental Salinity On the Cellular Profiles and Expression of Na+, K+-ATPase and Na+, K+, 2Cl- Cotransporter 1 of Branchial Mitochondrion-rich Cells in the Juvenile Marine Fish Monodactylus Argenteus." Fish Physiology and Biochemistry, vol. 38, no. 3, 2012, pp. 665-78.
Kang CK, Liu FC, Chang WB, et al. Effects of low environmental salinity on the cellular profiles and expression of Na+, K+-ATPase and Na+, K+, 2Cl- cotransporter 1 of branchial mitochondrion-rich cells in the juvenile marine fish Monodactylus argenteus. Fish Physiol Biochem. 2012;38(3):665-78.
Kang, C. K., Liu, F. C., Chang, W. B., & Lee, T. H. (2012). Effects of low environmental salinity on the cellular profiles and expression of Na+, K+-ATPase and Na+, K+, 2Cl- cotransporter 1 of branchial mitochondrion-rich cells in the juvenile marine fish Monodactylus argenteus. Fish Physiology and Biochemistry, 38(3), 665-78. https://doi.org/10.1007/s10695-011-9549-1
Kang CK, et al. Effects of Low Environmental Salinity On the Cellular Profiles and Expression of Na+, K+-ATPase and Na+, K+, 2Cl- Cotransporter 1 of Branchial Mitochondrion-rich Cells in the Juvenile Marine Fish Monodactylus Argenteus. Fish Physiol Biochem. 2012;38(3):665-78. PubMed PMID: 21863333.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of low environmental salinity on the cellular profiles and expression of Na+, K+-ATPase and Na+, K+, 2Cl- cotransporter 1 of branchial mitochondrion-rich cells in the juvenile marine fish Monodactylus argenteus. AU - Kang,Chao-Kai, AU - Liu,Fu-Chen, AU - Chang,Wen-Been, AU - Lee,Tsung-Han, Y1 - 2011/08/24/ PY - 2011/02/09/received PY - 2011/08/06/accepted PY - 2011/8/25/entrez PY - 2011/8/25/pubmed PY - 2012/9/18/medline SP - 665 EP - 78 JF - Fish physiology and biochemistry JO - Fish Physiol Biochem VL - 38 IS - 3 N2 - The goal of this study was to determine the osmoregulatory ability of a juvenile marine fish, silver moony (Monodactylus argenteus), for the purpose of developing a new experimental species for ecophysiological research. In this study, M. argenteus was acclimated to freshwater (FW), brackish water (BW), or seawater (SW). The salinity tolerance of this euryhaline species was effective, and the fish survived well upon osmotic challenges. The largest apical surface of mitochondrion-rich cells was found in the FW individuals. Immunohistochemical staining revealed that Na(+), K(+)-ATPase immunoreactive (NKA-IR) cells were distributed in the interlamellar region of the gill filaments of the silver moony in all experimental groups. In addition to the filaments, NKA-IR cells were also found in the lamellae of the FW individuals. The number of NKA-IR cells in the gills of the FW individuals exceeded that of the BW and SW individuals. The NKA-IR cells of FW and SW individuals exhibited bigger size than that of BW fish. The NKA activities and protein expression of the NKA α-subunit in the gills of the FW individuals were significantly higher than in the BW and SW groups. Additionally, the relative amounts of Na(+), K(+), 2Cl(-) cotransporter 1 (NKCC1) were salinity-dependent in the gills. Immunofluorescent signals of NKCC1 were localized to the basolateral membrane of NKA-IR cells in all groups. In the gills of the FW individuals, however, some NKA-IR cells did not exhibit a basolateral NKCC1 signal. In conclusion, the present study illustrated the osmoregulatory mechanisms of this easy- and economic-to-rear marine teleost with euryhaline capacity and proved the silver moony to be a good experimental animal. SN - 1573-5168 UR - https://www.unboundmedicine.com/medline/citation/21863333/Effects_of_low_environmental_salinity_on_the_cellular_profiles_and_expression_of_Na+_K+_ATPase_and_Na+_K+_2Cl__cotransporter_1_of_branchial_mitochondrion_rich_cells_in_the_juvenile_marine_fish_Monodactylus_argenteus_ L2 - https://dx.doi.org/10.1007/s10695-011-9549-1 DB - PRIME DP - Unbound Medicine ER -