Tags

Type your tag names separated by a space and hit enter

Increase in viral yield in eggs and MDCK cells of reassortant H5N1 vaccine candidate viruses caused by insertion of 38 amino acids into the NA stalk.
Vaccine. 2011 Oct 19; 29(45):8032-41.V

Abstract

BACKGROUND

The H5N1 subtype of highly pathogenic avian influenza viruses has spread to over 63 countries in Asia, Europe, and Africa and has become endemic in poultry. Since 2004, vaccination against H5N1 influenza has become common in domestic poultry operations in China. Most influenza vaccines have been produced in embryonated chicken eggs. High yield is the essential feature of a good vaccine candidate virus.

OBJECTIVE

Therefore, the large-scale manufacture of such a vaccine requires that the viral yield of H5N1 reassortant vaccine viruses in eggs and MDCK cells be increased.

METHODS

We generated two sets of reassortant H5N1 viruses based on backbone viruses A/Chicken/F/98 (H9N2) and A/Puerto Rico/8/34 (H1N1) using reverse genetics. The HAs and NAs of the reassortants were derived from the three epidemic H5N1 strains found in China. We compared the replication properties of these recombinant H5N1 viruses in embryonated chicken eggs and MDCK cells after inserting either 20 or 38 amino acids into their NA stalks.

RESULTS

In this study, we demonstrated that inserting 38 amino acids into the NA stalks can significantly increase the viral yield of H5N1 reassortant viruses in both embryonated chicken eggs and MDCK cells, while inserting only 20 amino acids into the same NA stalks does not. Hemagglutinin inhibition testing and protection assays indicated that recombinant H5N1 viruses with 38 aa inserted into their NA stalks had the same antigenicity as the viruses with wt-NA.

CONCLUSION

These results suggest that the generation of an H5N1 recombinant vaccine seed by the insertion of 38 aa into the NA stalk may be a suitable and more economical strategy for the increase in viral yield in both eggs and MDCK cells for the purposes of vaccine production.

Authors+Show Affiliations

Animal Infectious Disease Laboratory, School of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, Jiangsu 225009, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21864614

Citation

Zhang, Wenjun, et al. "Increase in Viral Yield in Eggs and MDCK Cells of Reassortant H5N1 Vaccine Candidate Viruses Caused By Insertion of 38 Amino Acids Into the NA Stalk." Vaccine, vol. 29, no. 45, 2011, pp. 8032-41.
Zhang W, Xue T, Wu X, et al. Increase in viral yield in eggs and MDCK cells of reassortant H5N1 vaccine candidate viruses caused by insertion of 38 amino acids into the NA stalk. Vaccine. 2011;29(45):8032-41.
Zhang, W., Xue, T., Wu, X., Zhang, P., Zhao, G., Peng, D., Hu, S., Wang, X., Liu, X., Liu, W., & Liu, X. (2011). Increase in viral yield in eggs and MDCK cells of reassortant H5N1 vaccine candidate viruses caused by insertion of 38 amino acids into the NA stalk. Vaccine, 29(45), 8032-41. https://doi.org/10.1016/j.vaccine.2011.08.054
Zhang W, et al. Increase in Viral Yield in Eggs and MDCK Cells of Reassortant H5N1 Vaccine Candidate Viruses Caused By Insertion of 38 Amino Acids Into the NA Stalk. Vaccine. 2011 Oct 19;29(45):8032-41. PubMed PMID: 21864614.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increase in viral yield in eggs and MDCK cells of reassortant H5N1 vaccine candidate viruses caused by insertion of 38 amino acids into the NA stalk. AU - Zhang,Wenjun, AU - Xue,Tao, AU - Wu,Xiaowei, AU - Zhang,Pinghu, AU - Zhao,Guo, AU - Peng,Daxing, AU - Hu,Shunlin, AU - Wang,Xiaoquan, AU - Liu,Xiaowen, AU - Liu,Wenbo, AU - Liu,Xiufan, Y1 - 2011/08/22/ PY - 2011/03/31/received PY - 2011/08/10/revised PY - 2011/08/10/accepted PY - 2011/8/26/entrez PY - 2011/8/26/pubmed PY - 2012/1/24/medline SP - 8032 EP - 41 JF - Vaccine JO - Vaccine VL - 29 IS - 45 N2 - BACKGROUND: The H5N1 subtype of highly pathogenic avian influenza viruses has spread to over 63 countries in Asia, Europe, and Africa and has become endemic in poultry. Since 2004, vaccination against H5N1 influenza has become common in domestic poultry operations in China. Most influenza vaccines have been produced in embryonated chicken eggs. High yield is the essential feature of a good vaccine candidate virus. OBJECTIVE: Therefore, the large-scale manufacture of such a vaccine requires that the viral yield of H5N1 reassortant vaccine viruses in eggs and MDCK cells be increased. METHODS: We generated two sets of reassortant H5N1 viruses based on backbone viruses A/Chicken/F/98 (H9N2) and A/Puerto Rico/8/34 (H1N1) using reverse genetics. The HAs and NAs of the reassortants were derived from the three epidemic H5N1 strains found in China. We compared the replication properties of these recombinant H5N1 viruses in embryonated chicken eggs and MDCK cells after inserting either 20 or 38 amino acids into their NA stalks. RESULTS: In this study, we demonstrated that inserting 38 amino acids into the NA stalks can significantly increase the viral yield of H5N1 reassortant viruses in both embryonated chicken eggs and MDCK cells, while inserting only 20 amino acids into the same NA stalks does not. Hemagglutinin inhibition testing and protection assays indicated that recombinant H5N1 viruses with 38 aa inserted into their NA stalks had the same antigenicity as the viruses with wt-NA. CONCLUSION: These results suggest that the generation of an H5N1 recombinant vaccine seed by the insertion of 38 aa into the NA stalk may be a suitable and more economical strategy for the increase in viral yield in both eggs and MDCK cells for the purposes of vaccine production. SN - 1873-2518 UR - https://www.unboundmedicine.com/medline/citation/21864614/Increase_in_viral_yield_in_eggs_and_MDCK_cells_of_reassortant_H5N1_vaccine_candidate_viruses_caused_by_insertion_of_38_amino_acids_into_the_NA_stalk_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(11)01295-3 DB - PRIME DP - Unbound Medicine ER -