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Phosphorylated α-synuclein can be detected in blood plasma and is potentially a useful biomarker for Parkinson's disease.
FASEB J. 2011 Dec; 25(12):4127-37.FJ

Abstract

Parkinson's disease (PD) is characterized by the presence of Lewy bodies containing phosphorylated and aggregated α-synuclein (α-syn). α-Syn is present in human body fluids, including blood plasma, and is a potential biomarker for PD. Immunoassays for total and oligomeric forms of both normal and phosphorylated (at Ser-129) α-syn have been used to assay plasma samples from a longitudinal cohort of 32 patients with PD (sampled at mo 0, 1, 2, 3), as well as single plasma samples from a group of 30 healthy control participants. The levels of α-syn in plasma varied greatly between individuals, but were remarkably consistent over time within the same individual with PD. The mean level of phospho-α-syn was found to be higher (P=0.053) in the PD samples than the controls, whereas this was not the case for total α-syn (P=0.244), oligo-α-syn (P=0.221), or oligo-phospho-α-syn (P=0.181). Immunoblots of plasma revealed bands (at 21, 24, and 50-60 kDa) corresponding to phosphorylated α-syn. Thus, phosphorylated α-syn can be detected in blood plasma and shows more promise as a diagnostic marker than the nonphosphorylated protein. Longitudinal studies undertaken over a more extended time period will be required to determine whether α-syn can act as a marker of disease progression.

Authors+Show Affiliations

Division of Biomedical and Life Sciences, School of Health and Medicine, University of Lancaster, Lancaster, LA1 4AY, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21865317

Citation

Foulds, Penelope G., et al. "Phosphorylated Α-synuclein Can Be Detected in Blood Plasma and Is Potentially a Useful Biomarker for Parkinson's Disease." FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, vol. 25, no. 12, 2011, pp. 4127-37.
Foulds PG, Mitchell JD, Parker A, et al. Phosphorylated α-synuclein can be detected in blood plasma and is potentially a useful biomarker for Parkinson's disease. FASEB J. 2011;25(12):4127-37.
Foulds, P. G., Mitchell, J. D., Parker, A., Turner, R., Green, G., Diggle, P., Hasegawa, M., Taylor, M., Mann, D., & Allsop, D. (2011). Phosphorylated α-synuclein can be detected in blood plasma and is potentially a useful biomarker for Parkinson's disease. FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, 25(12), 4127-37. https://doi.org/10.1096/fj.10-179192
Foulds PG, et al. Phosphorylated Α-synuclein Can Be Detected in Blood Plasma and Is Potentially a Useful Biomarker for Parkinson's Disease. FASEB J. 2011;25(12):4127-37. PubMed PMID: 21865317.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phosphorylated α-synuclein can be detected in blood plasma and is potentially a useful biomarker for Parkinson's disease. AU - Foulds,Penelope G, AU - Mitchell,J Douglas, AU - Parker,Angela, AU - Turner,Roisin, AU - Green,Gerwyn, AU - Diggle,Peter, AU - Hasegawa,Masato, AU - Taylor,Mark, AU - Mann,David, AU - Allsop,David, Y1 - 2011/08/24/ PY - 2011/8/26/entrez PY - 2011/8/26/pubmed PY - 2012/3/1/medline SP - 4127 EP - 37 JF - FASEB journal : official publication of the Federation of American Societies for Experimental Biology JO - FASEB J VL - 25 IS - 12 N2 - Parkinson's disease (PD) is characterized by the presence of Lewy bodies containing phosphorylated and aggregated α-synuclein (α-syn). α-Syn is present in human body fluids, including blood plasma, and is a potential biomarker for PD. Immunoassays for total and oligomeric forms of both normal and phosphorylated (at Ser-129) α-syn have been used to assay plasma samples from a longitudinal cohort of 32 patients with PD (sampled at mo 0, 1, 2, 3), as well as single plasma samples from a group of 30 healthy control participants. The levels of α-syn in plasma varied greatly between individuals, but were remarkably consistent over time within the same individual with PD. The mean level of phospho-α-syn was found to be higher (P=0.053) in the PD samples than the controls, whereas this was not the case for total α-syn (P=0.244), oligo-α-syn (P=0.221), or oligo-phospho-α-syn (P=0.181). Immunoblots of plasma revealed bands (at 21, 24, and 50-60 kDa) corresponding to phosphorylated α-syn. Thus, phosphorylated α-syn can be detected in blood plasma and shows more promise as a diagnostic marker than the nonphosphorylated protein. Longitudinal studies undertaken over a more extended time period will be required to determine whether α-syn can act as a marker of disease progression. SN - 1530-6860 UR - https://www.unboundmedicine.com/medline/citation/21865317/Phosphorylated_α_synuclein_can_be_detected_in_blood_plasma_and_is_potentially_a_useful_biomarker_for_Parkinson's_disease_ L2 - https://doi.org/10.1096/fj.10-179192 DB - PRIME DP - Unbound Medicine ER -