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Phosphorylated α-synuclein can be detected in blood plasma and is potentially a useful biomarker for Parkinson's disease.
FASEB J. 2011 Dec; 25(12):4127-37.FJ

Abstract

Parkinson's disease (PD) is characterized by the presence of Lewy bodies containing phosphorylated and aggregated α-synuclein (α-syn). α-Syn is present in human body fluids, including blood plasma, and is a potential biomarker for PD. Immunoassays for total and oligomeric forms of both normal and phosphorylated (at Ser-129) α-syn have been used to assay plasma samples from a longitudinal cohort of 32 patients with PD (sampled at mo 0, 1, 2, 3), as well as single plasma samples from a group of 30 healthy control participants. The levels of α-syn in plasma varied greatly between individuals, but were remarkably consistent over time within the same individual with PD. The mean level of phospho-α-syn was found to be higher (P=0.053) in the PD samples than the controls, whereas this was not the case for total α-syn (P=0.244), oligo-α-syn (P=0.221), or oligo-phospho-α-syn (P=0.181). Immunoblots of plasma revealed bands (at 21, 24, and 50-60 kDa) corresponding to phosphorylated α-syn. Thus, phosphorylated α-syn can be detected in blood plasma and shows more promise as a diagnostic marker than the nonphosphorylated protein. Longitudinal studies undertaken over a more extended time period will be required to determine whether α-syn can act as a marker of disease progression.

Links

Publisher Full Text

Authors+Show Affiliations

Foulds PG
Division of Biomedical and Life Sciences, School of Health and Medicine, University of Lancaster, Lancaster, LA1 4AY, UK.
Mitchell JD
No affiliation info available
Parker A
No affiliation info available
Turner R
No affiliation info available
Green G
No affiliation info available
Diggle P
No affiliation info available
Hasegawa M
No affiliation info available
Taylor M
No affiliation info available
Mann D
No affiliation info available
Allsop D
No affiliation info available

MeSH

AgedBiomarkersCase-Control StudiesCohort StudiesFemaleHumansImmunoblottingLongitudinal StudiesMaleMiddle AgedParkinson DiseasePhosphorylationROC Curvealpha-Synuclein

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21865317

Clinical Trial Links

Establishing Alpha-synuclein RT-QuIC Assay as a Diagnostic Technique in REM Sleep Behaviour Disorder

Citation

Foulds, Penelope G., et al. "Phosphorylated Α-synuclein Can Be Detected in Blood Plasma and Is Potentially a Useful Biomarker for Parkinson's Disease." FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, vol. 25, no. 12, 2011, pp. 4127-37.
Foulds PG, Mitchell JD, Parker A, et al. Phosphorylated α-synuclein can be detected in blood plasma and is potentially a useful biomarker for Parkinson's disease. FASEB J. 2011;25(12):4127-37.
Foulds, P. G., Mitchell, J. D., Parker, A., Turner, R., Green, G., Diggle, P., Hasegawa, M., Taylor, M., Mann, D., & Allsop, D. (2011). Phosphorylated α-synuclein can be detected in blood plasma and is potentially a useful biomarker for Parkinson's disease. FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, 25(12), 4127-37. https://doi.org/10.1096/fj.10-179192
Foulds PG, et al. Phosphorylated Α-synuclein Can Be Detected in Blood Plasma and Is Potentially a Useful Biomarker for Parkinson's Disease. FASEB J. 2011;25(12):4127-37. PubMed PMID: 21865317.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phosphorylated α-synuclein can be detected in blood plasma and is potentially a useful biomarker for Parkinson's disease. AU - Foulds,Penelope G, AU - Mitchell,J Douglas, AU - Parker,Angela, AU - Turner,Roisin, AU - Green,Gerwyn, AU - Diggle,Peter, AU - Hasegawa,Masato, AU - Taylor,Mark, AU - Mann,David, AU - Allsop,David, Y1 - 2011/08/24/ PY - 2011/8/26/entrez PY - 2011/8/26/pubmed PY - 2012/3/1/medline SP - 4127 EP - 37 JF - FASEB journal : official publication of the Federation of American Societies for Experimental Biology JO - FASEB J VL - 25 IS - 12 N2 - Parkinson's disease (PD) is characterized by the presence of Lewy bodies containing phosphorylated and aggregated α-synuclein (α-syn). α-Syn is present in human body fluids, including blood plasma, and is a potential biomarker for PD. Immunoassays for total and oligomeric forms of both normal and phosphorylated (at Ser-129) α-syn have been used to assay plasma samples from a longitudinal cohort of 32 patients with PD (sampled at mo 0, 1, 2, 3), as well as single plasma samples from a group of 30 healthy control participants. The levels of α-syn in plasma varied greatly between individuals, but were remarkably consistent over time within the same individual with PD. The mean level of phospho-α-syn was found to be higher (P=0.053) in the PD samples than the controls, whereas this was not the case for total α-syn (P=0.244), oligo-α-syn (P=0.221), or oligo-phospho-α-syn (P=0.181). Immunoblots of plasma revealed bands (at 21, 24, and 50-60 kDa) corresponding to phosphorylated α-syn. Thus, phosphorylated α-syn can be detected in blood plasma and shows more promise as a diagnostic marker than the nonphosphorylated protein. Longitudinal studies undertaken over a more extended time period will be required to determine whether α-syn can act as a marker of disease progression. SN - 1530-6860 UR - https://www.unboundmedicine.com/medline/citation/21865317/Phosphorylated_α_synuclein_can_be_detected_in_blood_plasma_and_is_potentially_a_useful_biomarker_for_Parkinson's_disease_ L2 - https://doi.org/10.1096/fj.10-179192 DB - PRIME DP - Unbound Medicine ER -