Abstract
Nasal vaccination is an effective therapeutic regimen for preventing upper respiratory infectious diseases. In the development of nasal vaccine, an appropriate adjuvant is required. In the present study, we examined the efficacy of fms-like tyrosine kinase receptor-3 ligand (Flt3L) as a mucosal adjuvant. Mice were immunized intranasally with Flt3L and P6 protein of nontypeable Haemophilus influenzae (NTHi), and P6-specific immune responses were examined. In addition, NTHi challenges were performed and the level of NTHi was quantified in nasal washes. Nasal vaccination with P6 and Flt3L induced an increase in the number of dendritic cells in nasal-associated lymphoid tissue. P6-specific nasal wash immunoglobulin (Ig)A and serum IgG titers were elevated significantly after nasal immunization. Enhanced NTHi clearance from the nasopharynx was also observed. These results indicate the potential of Flt3L as an effective mucosal adjuvant and suggest that nasal vaccination with P6 and Flt3L might be an effective regimen for the induction of NTHi-specific protective immunity.
TY - JOUR
T1 - Nasal-associated lymphoid tissue immunity and vaccine development.
AU - Kodama,Satoru,
AU - Suzuki,Masashi,
Y1 - 2011/08/18/
PY - 2011/8/26/entrez
PY - 2011/8/26/pubmed
PY - 2012/1/18/medline
SP - 110
EP - 2
JF - Advances in oto-rhino-laryngology
JO - Adv Otorhinolaryngol
VL - 72
N2 - Nasal vaccination is an effective therapeutic regimen for preventing upper respiratory infectious diseases. In the development of nasal vaccine, an appropriate adjuvant is required. In the present study, we examined the efficacy of fms-like tyrosine kinase receptor-3 ligand (Flt3L) as a mucosal adjuvant. Mice were immunized intranasally with Flt3L and P6 protein of nontypeable Haemophilus influenzae (NTHi), and P6-specific immune responses were examined. In addition, NTHi challenges were performed and the level of NTHi was quantified in nasal washes. Nasal vaccination with P6 and Flt3L induced an increase in the number of dendritic cells in nasal-associated lymphoid tissue. P6-specific nasal wash immunoglobulin (Ig)A and serum IgG titers were elevated significantly after nasal immunization. Enhanced NTHi clearance from the nasopharynx was also observed. These results indicate the potential of Flt3L as an effective mucosal adjuvant and suggest that nasal vaccination with P6 and Flt3L might be an effective regimen for the induction of NTHi-specific protective immunity.
SN - 1662-2847
UR - https://www.unboundmedicine.com/medline/citation/21865705/Nasal_associated_lymphoid_tissue_immunity_and_vaccine_development_
L2 - https://www.karger.com?DOI=10.1159/000324635
DB - PRIME
DP - Unbound Medicine
ER -
