Tags

Type your tag names separated by a space and hit enter

Effects of serine protease inhibitors on growth and development and digestive serine proteinases of the Sunn pest, Eurygaster integriceps.
J Insect Sci. 2011; 11:72.JI

Abstract

In the current study the effects of serine proteinase inhibitors (TLCK, TPCK, SBTI, and a combination of SBTI and TPCK) with concentrations of 1% and 4% of dietary protein in artificial diets were tested against growth of the Sunn pest, Eurygaster integriceps Puton (Hemiptera: Scutelleridae), development, and its gut serine proteinase targets. Analysis of variance indicated that protease inhibitors affected nymphal development time, adult weight, and survival. Mean development time of third instar nymphs in control, SBTI (1%), TLCK (1%), and TPCK was 7.18, 9.74, 9.97, and 8.52 days, respectively. The highest mortality (100 % mortality) was observed when a combination of TPCK and SBTI, both at 4% of dietary protein, was used followed by TPCK (4%) that produced 95% mortality. There were significant differences in proteinase activity between treatments and controls when BApNA and SAAPFpNA were used as substrates for trypsin and chymotrypsin, respectively. Reduction of trypsin activity in insects fed with low doses of SBTI (1%), TLCK (1%), and both doses of TPCK (1% and 4%) was 40, 26, 23, and 17%, respectively. Inhibition of chymotrypsin activity was seen in the insects fed on SBTI (1%), TLCK (1%), and TPCK (4%) where inhibition was 14, 9, and 36%, respectively. Maximum inhibition of chymotrypsin activity was observed in the insects fed on diets containing high doses of TPCK (4%). In gel assays, the greatest effects were observed when E. integriceps were fed on high doses of SBTI and TPCK. Therefore, TPCK followed by SBTI proved to be the most effective proteinase inhibitors of E. integriceps.

Authors+Show Affiliations

Plant Protection Department, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. F.saadaty@yahoo.comNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21867440

Citation

Saadati, Fatemeh, and Ali R. Bandani. "Effects of Serine Protease Inhibitors On Growth and Development and Digestive Serine Proteinases of the Sunn Pest, Eurygaster Integriceps." Journal of Insect Science (Online), vol. 11, 2011, p. 72.
Saadati F, Bandani AR. Effects of serine protease inhibitors on growth and development and digestive serine proteinases of the Sunn pest, Eurygaster integriceps. J Insect Sci. 2011;11:72.
Saadati, F., & Bandani, A. R. (2011). Effects of serine protease inhibitors on growth and development and digestive serine proteinases of the Sunn pest, Eurygaster integriceps. Journal of Insect Science (Online), 11, 72. https://doi.org/10.1673/031.011.7201
Saadati F, Bandani AR. Effects of Serine Protease Inhibitors On Growth and Development and Digestive Serine Proteinases of the Sunn Pest, Eurygaster Integriceps. J Insect Sci. 2011;11:72. PubMed PMID: 21867440.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of serine protease inhibitors on growth and development and digestive serine proteinases of the Sunn pest, Eurygaster integriceps. AU - Saadati,Fatemeh, AU - Bandani,Ali R, PY - 2011/8/27/entrez PY - 2011/8/27/pubmed PY - 2011/11/15/medline SP - 72 EP - 72 JF - Journal of insect science (Online) JO - J Insect Sci VL - 11 N2 - In the current study the effects of serine proteinase inhibitors (TLCK, TPCK, SBTI, and a combination of SBTI and TPCK) with concentrations of 1% and 4% of dietary protein in artificial diets were tested against growth of the Sunn pest, Eurygaster integriceps Puton (Hemiptera: Scutelleridae), development, and its gut serine proteinase targets. Analysis of variance indicated that protease inhibitors affected nymphal development time, adult weight, and survival. Mean development time of third instar nymphs in control, SBTI (1%), TLCK (1%), and TPCK was 7.18, 9.74, 9.97, and 8.52 days, respectively. The highest mortality (100 % mortality) was observed when a combination of TPCK and SBTI, both at 4% of dietary protein, was used followed by TPCK (4%) that produced 95% mortality. There were significant differences in proteinase activity between treatments and controls when BApNA and SAAPFpNA were used as substrates for trypsin and chymotrypsin, respectively. Reduction of trypsin activity in insects fed with low doses of SBTI (1%), TLCK (1%), and both doses of TPCK (1% and 4%) was 40, 26, 23, and 17%, respectively. Inhibition of chymotrypsin activity was seen in the insects fed on SBTI (1%), TLCK (1%), and TPCK (4%) where inhibition was 14, 9, and 36%, respectively. Maximum inhibition of chymotrypsin activity was observed in the insects fed on diets containing high doses of TPCK (4%). In gel assays, the greatest effects were observed when E. integriceps were fed on high doses of SBTI and TPCK. Therefore, TPCK followed by SBTI proved to be the most effective proteinase inhibitors of E. integriceps. SN - 1536-2442 UR - https://www.unboundmedicine.com/medline/citation/21867440/Effects_of_serine_protease_inhibitors_on_growth_and_development_and_digestive_serine_proteinases_of_the_Sunn_pest_Eurygaster_integriceps_ L2 - https://academic.oup.com/jinsectscience/article-lookup/doi/10.1673/031.011.7201 DB - PRIME DP - Unbound Medicine ER -