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Molecular detection and sequence analysis of hepatitis E virus in patients with viral hepatitis from North India.
Diagn Microbiol Infect Dis. 2011 Oct; 71(2):110-7.DM

Abstract

Viral hepatitis is a major cause of mortality and morbidity in developing countries. Hepatitis E virus (HEV) is responsible for both sporadic and epidemic outbreaks of viral hepatitis in India. Here a total of 843 samples were collected: 685 from patients with acute viral hepatitis (AVH), 70 from patients with fulminant hepatic failure (FHF), 53 from patients with chronic liver disease (CLD), 11 from patients with antituberculosis therapy (ATT)-induced jaundice, and 24 from pregnant women. When tested for anti-HEV IgM, 58.3% of the pregnant women, 41.4% of the patients with FHF, 38.6% of the patients with AVH, 9.4% of the patients with CLD, and 18.2% of the patients with ATT-induced jaundice tested positive. We found that 34% and 16% of the acute hepatitis patients and fulminant hepatitis patients, respectively, showed no reactivity to the existing viral hepatitis markers and were thus grouped as non A to E. Among the HEV IgM-positive cases, males outnumbered females (62.8% versus 37.1%). HEV RNA was found in 35% of fulminant and 9.4% of acute hepatitis patients. From phylogenetic analysis, we observed that all the isolates were clustered within genotype 1. Critical analysis placed the acute isolates along with strains under subtype Ia, while the fulminant isolates clustered along with the FHF strain (X98292) under subtype Ic. The segregation of HEV isolates from AVH and FHF patients into different subtypes raises interesting questions on the molecular basis of HEV disease severity.

Authors+Show Affiliations

College of Veterinary Medicine, University of Maryland, MD 20742, USA. subrat_kumar@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21868185

Citation

Kumar, Subrat, et al. "Molecular Detection and Sequence Analysis of Hepatitis E Virus in Patients With Viral Hepatitis From North India." Diagnostic Microbiology and Infectious Disease, vol. 71, no. 2, 2011, pp. 110-7.
Kumar S, Pujhari SK, Chawla YK, et al. Molecular detection and sequence analysis of hepatitis E virus in patients with viral hepatitis from North India. Diagn Microbiol Infect Dis. 2011;71(2):110-7.
Kumar, S., Pujhari, S. K., Chawla, Y. K., Chakraborti, A., & Ratho, R. K. (2011). Molecular detection and sequence analysis of hepatitis E virus in patients with viral hepatitis from North India. Diagnostic Microbiology and Infectious Disease, 71(2), 110-7. https://doi.org/10.1016/j.diagmicrobio.2011.06.019
Kumar S, et al. Molecular Detection and Sequence Analysis of Hepatitis E Virus in Patients With Viral Hepatitis From North India. Diagn Microbiol Infect Dis. 2011;71(2):110-7. PubMed PMID: 21868185.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular detection and sequence analysis of hepatitis E virus in patients with viral hepatitis from North India. AU - Kumar,Subrat, AU - Pujhari,Sujit Kumar, AU - Chawla,Yogesh Kumar, AU - Chakraborti,Anuradha, AU - Ratho,Radha Kanta, Y1 - 2011/08/24/ PY - 2011/03/22/received PY - 2011/06/23/revised PY - 2011/06/29/accepted PY - 2011/8/27/entrez PY - 2011/8/27/pubmed PY - 2012/1/24/medline SP - 110 EP - 7 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 71 IS - 2 N2 - Viral hepatitis is a major cause of mortality and morbidity in developing countries. Hepatitis E virus (HEV) is responsible for both sporadic and epidemic outbreaks of viral hepatitis in India. Here a total of 843 samples were collected: 685 from patients with acute viral hepatitis (AVH), 70 from patients with fulminant hepatic failure (FHF), 53 from patients with chronic liver disease (CLD), 11 from patients with antituberculosis therapy (ATT)-induced jaundice, and 24 from pregnant women. When tested for anti-HEV IgM, 58.3% of the pregnant women, 41.4% of the patients with FHF, 38.6% of the patients with AVH, 9.4% of the patients with CLD, and 18.2% of the patients with ATT-induced jaundice tested positive. We found that 34% and 16% of the acute hepatitis patients and fulminant hepatitis patients, respectively, showed no reactivity to the existing viral hepatitis markers and were thus grouped as non A to E. Among the HEV IgM-positive cases, males outnumbered females (62.8% versus 37.1%). HEV RNA was found in 35% of fulminant and 9.4% of acute hepatitis patients. From phylogenetic analysis, we observed that all the isolates were clustered within genotype 1. Critical analysis placed the acute isolates along with strains under subtype Ia, while the fulminant isolates clustered along with the FHF strain (X98292) under subtype Ic. The segregation of HEV isolates from AVH and FHF patients into different subtypes raises interesting questions on the molecular basis of HEV disease severity. SN - 1879-0070 UR - https://www.unboundmedicine.com/medline/citation/21868185/Molecular_detection_and_sequence_analysis_of_hepatitis_E_virus_in_patients_with_viral_hepatitis_from_North_India_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(11)00259-8 DB - PRIME DP - Unbound Medicine ER -