Tags

Type your tag names separated by a space and hit enter

Expansion of European vacA and cagA alleles to East-Asian Helicobacter pylori strains in Cambodia.
Infect Genet Evol. 2011 Dec; 11(8):1899-905.IG

Abstract

Helicobacter pylori infection is associated with gastric cancer (GC). The highest incidence rates have been described in Asia, but regional variations exist that do not match the distribution of infection prevalence rates. The aim of the study was to examine the possible contribution of H. pylori virulence factors to geographic differences in the incidence of GC across East and Southeast Asia. We studied 66 isolates from Cambodian patients that had previously been assigned to two genetic populations based on sequences of seven housekeeping genes, namely hpEurope (n = 34, 51.5%) and hpEastAsia, subpopulation hspEAsia (n = 32, 48.5%). These strains were characterized with respect to vacA polymorphism and cagA status by PCR, and the CagA C-terminal region was sequenced. We also sequenced the complete cagA gene from 10 hpEurope and 10 hspEAsia strains chosen at random. The cagA gene was present in 92.4% of the 66 isolates and was mainly of Western type (n = 36, 59.0%). hspEAsia strains carrying East-Asian CagA and the m1-type vacA allele (15.2%) were less frequent among the 66 Cambodian isolates than reported in East Asian countries, a finding that might partly explain the intermediate incidence of GC in Cambodia, and by extension, in Southeast Asia (except for Vietnam). The observed high prevalence of s1a alleles (34.4%) and Western CagA (28.1%) among hspEAsia strains indicates frequent introgression of European vacA and cagA alleles into East Asian H. pylori strains. This expansion might have severe consequences for individual disease outcome.

Authors+Show Affiliations

Unité de Bactériologie Médicale et Environnementale, Institut Pasteur, 36 Avenue Pasteur, BP 220, Dakar, Senegal. sbreurec@pasteur.snNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21871583

Citation

Breurec, Sebastien, et al. "Expansion of European vacA and cagA Alleles to East-Asian Helicobacter Pylori Strains in Cambodia." Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, vol. 11, no. 8, 2011, pp. 1899-905.
Breurec S, Guillard B, Hem S, et al. Expansion of European vacA and cagA alleles to East-Asian Helicobacter pylori strains in Cambodia. Infect Genet Evol. 2011;11(8):1899-905.
Breurec, S., Guillard, B., Hem, S., Papadakos, K. S., Brisse, S., Huerre, M., Monchy, D., Oung, C., Sgouras, D. N., Tan, T. S., Thiberge, J. M., Vong, S., Raymond, J., & Linz, B. (2011). Expansion of European vacA and cagA alleles to East-Asian Helicobacter pylori strains in Cambodia. Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, 11(8), 1899-905. https://doi.org/10.1016/j.meegid.2011.08.007
Breurec S, et al. Expansion of European vacA and cagA Alleles to East-Asian Helicobacter Pylori Strains in Cambodia. Infect Genet Evol. 2011;11(8):1899-905. PubMed PMID: 21871583.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expansion of European vacA and cagA alleles to East-Asian Helicobacter pylori strains in Cambodia. AU - Breurec,Sebastien, AU - Guillard,Bertrand, AU - Hem,Sopheak, AU - Papadakos,Konstantinos S, AU - Brisse,Sylvain, AU - Huerre,Michel, AU - Monchy,Didier, AU - Oung,Chakravuth, AU - Sgouras,Dionyssios N, AU - Tan,Tek Sreng, AU - Thiberge,Jean-Michel, AU - Vong,Sirenda, AU - Raymond,Josette, AU - Linz,Bodo, Y1 - 2011/08/17/ PY - 2011/05/04/received PY - 2011/08/04/revised PY - 2011/08/07/accepted PY - 2011/8/30/entrez PY - 2011/8/30/pubmed PY - 2012/3/27/medline SP - 1899 EP - 905 JF - Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases JO - Infect Genet Evol VL - 11 IS - 8 N2 - Helicobacter pylori infection is associated with gastric cancer (GC). The highest incidence rates have been described in Asia, but regional variations exist that do not match the distribution of infection prevalence rates. The aim of the study was to examine the possible contribution of H. pylori virulence factors to geographic differences in the incidence of GC across East and Southeast Asia. We studied 66 isolates from Cambodian patients that had previously been assigned to two genetic populations based on sequences of seven housekeeping genes, namely hpEurope (n = 34, 51.5%) and hpEastAsia, subpopulation hspEAsia (n = 32, 48.5%). These strains were characterized with respect to vacA polymorphism and cagA status by PCR, and the CagA C-terminal region was sequenced. We also sequenced the complete cagA gene from 10 hpEurope and 10 hspEAsia strains chosen at random. The cagA gene was present in 92.4% of the 66 isolates and was mainly of Western type (n = 36, 59.0%). hspEAsia strains carrying East-Asian CagA and the m1-type vacA allele (15.2%) were less frequent among the 66 Cambodian isolates than reported in East Asian countries, a finding that might partly explain the intermediate incidence of GC in Cambodia, and by extension, in Southeast Asia (except for Vietnam). The observed high prevalence of s1a alleles (34.4%) and Western CagA (28.1%) among hspEAsia strains indicates frequent introgression of European vacA and cagA alleles into East Asian H. pylori strains. This expansion might have severe consequences for individual disease outcome. SN - 1567-7257 UR - https://www.unboundmedicine.com/medline/citation/21871583/Expansion_of_European_vacA_and_cagA_alleles_to_East_Asian_Helicobacter_pylori_strains_in_Cambodia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-1348(11)00286-3 DB - PRIME DP - Unbound Medicine ER -