Tags

Type your tag names separated by a space and hit enter

Protective effects of melittin on transforming growth factor-β1 injury to hepatocytes via anti-apoptotic mechanism.
Toxicol Appl Pharmacol 2011; 256(2):209-15TA

Abstract

Melittin is a cationic, hemolytic peptide that is the main toxic component in the venom of the honey bee (Apis mellifera). Melittin has multiple effects, including anti-bacterial, anti-viral and anti-inflammatory, in various cell types. However, the anti-apoptotic mechanisms of melittin have not been fully elucidated in hepatocytes. Apoptosis contributes to liver inflammation and fibrosis. Knowledge of the apoptotic mechanisms is important to develop new and effective therapies for treatment of cirrhosis, portal hypertension, liver cancer, and other liver diseases. In the present study, we investigated the anti-apoptotic effect of melittin on transforming growth factor (TGF)-β1-induced apoptosis in hepatocytes. TGF-β1-treated hepatocytes were exposed to low doses (0.5 and 1 μg/mL) and high dose (2 μg/mL) of melittin. The low doses significantly protected these cells from DNA damage in TGF-β1-induced apoptosis compared to the high dose. Also, melittin suppressed TGF-β1-induced apoptotic activation of the Bcl-2 family and caspase family of proteins, which resulted in the inhibition of poly-ADP-ribose polymerase (PARP) cleavage. These results demonstrate that TGF-β1 induces hepatocyte apoptosis and that an optimal dose of melittin exerts anti-apoptotic effects against TGF-β1-induced injury to hepatocytes via the mitochondrial pathway. These results suggest that an optimal dose of melittin can serve to protect cells against TGF-β1-mediated injury.

Authors+Show Affiliations

Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21871910

Citation

Lee, Woo-Ram, et al. "Protective Effects of Melittin On Transforming Growth Factor-β1 Injury to Hepatocytes Via Anti-apoptotic Mechanism." Toxicology and Applied Pharmacology, vol. 256, no. 2, 2011, pp. 209-15.
Lee WR, Park JH, Kim KH, et al. Protective effects of melittin on transforming growth factor-β1 injury to hepatocytes via anti-apoptotic mechanism. Toxicol Appl Pharmacol. 2011;256(2):209-15.
Lee, W. R., Park, J. H., Kim, K. H., Park, Y. Y., Han, S. M., & Park, K. K. (2011). Protective effects of melittin on transforming growth factor-β1 injury to hepatocytes via anti-apoptotic mechanism. Toxicology and Applied Pharmacology, 256(2), pp. 209-15. doi:10.1016/j.taap.2011.08.012.
Lee WR, et al. Protective Effects of Melittin On Transforming Growth Factor-β1 Injury to Hepatocytes Via Anti-apoptotic Mechanism. Toxicol Appl Pharmacol. 2011 Oct 15;256(2):209-15. PubMed PMID: 21871910.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of melittin on transforming growth factor-β1 injury to hepatocytes via anti-apoptotic mechanism. AU - Lee,Woo-Ram, AU - Park,Ji-Hyun, AU - Kim,Kyung-Hyun, AU - Park,Yoon-Yub, AU - Han,Sang-Mi, AU - Park,Kwan-kyu, Y1 - 2011/08/17/ PY - 2011/07/22/received PY - 2011/08/10/revised PY - 2011/08/10/accepted PY - 2011/8/30/entrez PY - 2011/8/30/pubmed PY - 2011/12/13/medline SP - 209 EP - 15 JF - Toxicology and applied pharmacology JO - Toxicol. Appl. Pharmacol. VL - 256 IS - 2 N2 - Melittin is a cationic, hemolytic peptide that is the main toxic component in the venom of the honey bee (Apis mellifera). Melittin has multiple effects, including anti-bacterial, anti-viral and anti-inflammatory, in various cell types. However, the anti-apoptotic mechanisms of melittin have not been fully elucidated in hepatocytes. Apoptosis contributes to liver inflammation and fibrosis. Knowledge of the apoptotic mechanisms is important to develop new and effective therapies for treatment of cirrhosis, portal hypertension, liver cancer, and other liver diseases. In the present study, we investigated the anti-apoptotic effect of melittin on transforming growth factor (TGF)-β1-induced apoptosis in hepatocytes. TGF-β1-treated hepatocytes were exposed to low doses (0.5 and 1 μg/mL) and high dose (2 μg/mL) of melittin. The low doses significantly protected these cells from DNA damage in TGF-β1-induced apoptosis compared to the high dose. Also, melittin suppressed TGF-β1-induced apoptotic activation of the Bcl-2 family and caspase family of proteins, which resulted in the inhibition of poly-ADP-ribose polymerase (PARP) cleavage. These results demonstrate that TGF-β1 induces hepatocyte apoptosis and that an optimal dose of melittin exerts anti-apoptotic effects against TGF-β1-induced injury to hepatocytes via the mitochondrial pathway. These results suggest that an optimal dose of melittin can serve to protect cells against TGF-β1-mediated injury. SN - 1096-0333 UR - https://www.unboundmedicine.com/medline/citation/21871910/Protective_effects_of_melittin_on_transforming_growth_factor_β1_injury_to_hepatocytes_via_anti_apoptotic_mechanism_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-008X(11)00310-3 DB - PRIME DP - Unbound Medicine ER -