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Free radicals and antioxidants at a glance using EPR spectroscopy.
Crit Rev Clin Lab Sci 2011 May-Jun; 48(3):114-42CR

Abstract

The delicate balance between the advantageous and detrimental effects of free radicals is one of the important aspects of human (patho)physiology. The controlled production of reactive oxygen and nitrogen species has an essential role in the regulation of various signaling switches. On the other hand, imbalanced generation of radicals is highly correlated with the pathogenesis of many diseases which require the application of selected antioxidants to regain the homeostasis. In the era of growing interest for redox processes, electron paramagnetic resonance (EPR) spectroscopy is arguably the best-suited technique for such research due to its ability to provide a unique insight into the world of free radicals and antioxidants. Herein, I present the principles of EPR spectroscopy and the applications of this method in assessing: (i) the oxidative status of biological systems, using endogenous long-lived free radicals (ascorbyl radical (Asc(•)), tocopheroxyl radical (TO(•)), melanin) as markers; (ii) the production of short-lived radicals (hydroxyl radical (OH(•)), superoxide radical anion (O(2)(•-)), sulfur- and carbon-centered radicals), which are implicated in both, oxidative stress and redox signaling; (iii) the metabolism of nitric oxide (NO(•)); (iv) the antioxidative properties of various drugs, compounds, and natural products; (v) other redox-relevant parameter. Besides giving a comprehensive survey of up-to-date literature, I also provide illustrative examples in sufficient detail to provide a means to exploit the potential of EPR in biochemical/physiological/medical research. The emphasis is on the features and characteristics (both positive and negative) relevant for EPR application in clinical sciences. My aim is to encourage fellow colleagues interested in free radicals and antioxidants to expand their base knowledge or methods used in their laboratories with data acquired by EPR or some of the EPR techniques outlined in this review, in order to boost up the exciting area of redox science.

Authors+Show Affiliations

Institute for Multidisciplinary Research, University of Belgrade, Belgrade, Serbia. ivan@cms.bg.ac.rs

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

21875311

Citation

Spasojević, Ivan. "Free Radicals and Antioxidants at a Glance Using EPR Spectroscopy." Critical Reviews in Clinical Laboratory Sciences, vol. 48, no. 3, 2011, pp. 114-42.
Spasojević I. Free radicals and antioxidants at a glance using EPR spectroscopy. Crit Rev Clin Lab Sci. 2011;48(3):114-42.
Spasojević, I. (2011). Free radicals and antioxidants at a glance using EPR spectroscopy. Critical Reviews in Clinical Laboratory Sciences, 48(3), pp. 114-42. doi:10.3109/10408363.2011.591772.
Spasojević I. Free Radicals and Antioxidants at a Glance Using EPR Spectroscopy. Crit Rev Clin Lab Sci. 2011;48(3):114-42. PubMed PMID: 21875311.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Free radicals and antioxidants at a glance using EPR spectroscopy. A1 - Spasojević,Ivan, PY - 2011/8/31/entrez PY - 2011/8/31/pubmed PY - 2011/12/13/medline SP - 114 EP - 42 JF - Critical reviews in clinical laboratory sciences JO - Crit Rev Clin Lab Sci VL - 48 IS - 3 N2 - The delicate balance between the advantageous and detrimental effects of free radicals is one of the important aspects of human (patho)physiology. The controlled production of reactive oxygen and nitrogen species has an essential role in the regulation of various signaling switches. On the other hand, imbalanced generation of radicals is highly correlated with the pathogenesis of many diseases which require the application of selected antioxidants to regain the homeostasis. In the era of growing interest for redox processes, electron paramagnetic resonance (EPR) spectroscopy is arguably the best-suited technique for such research due to its ability to provide a unique insight into the world of free radicals and antioxidants. Herein, I present the principles of EPR spectroscopy and the applications of this method in assessing: (i) the oxidative status of biological systems, using endogenous long-lived free radicals (ascorbyl radical (Asc(•)), tocopheroxyl radical (TO(•)), melanin) as markers; (ii) the production of short-lived radicals (hydroxyl radical (OH(•)), superoxide radical anion (O(2)(•-)), sulfur- and carbon-centered radicals), which are implicated in both, oxidative stress and redox signaling; (iii) the metabolism of nitric oxide (NO(•)); (iv) the antioxidative properties of various drugs, compounds, and natural products; (v) other redox-relevant parameter. Besides giving a comprehensive survey of up-to-date literature, I also provide illustrative examples in sufficient detail to provide a means to exploit the potential of EPR in biochemical/physiological/medical research. The emphasis is on the features and characteristics (both positive and negative) relevant for EPR application in clinical sciences. My aim is to encourage fellow colleagues interested in free radicals and antioxidants to expand their base knowledge or methods used in their laboratories with data acquired by EPR or some of the EPR techniques outlined in this review, in order to boost up the exciting area of redox science. SN - 1549-781X UR - https://www.unboundmedicine.com/medline/citation/21875311/Free_radicals_and_antioxidants_at_a_glance_using_EPR_spectroscopy_ L2 - http://www.tandfonline.com/doi/full/10.3109/10408363.2011.591772 DB - PRIME DP - Unbound Medicine ER -