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Awakening responses and diurnal fluctuations of salivary cortisol, DHEA-S and α-amylase in healthy male subjects.
Neuro Endocrinol Lett. 2011; 32(4):475-80.NE

Abstract

OBJECTIVES

Because the cortisol awakening response (CAR) has received increasing attention as a useful index of adrenocortical activity, the primary objective of this study was to investigate the presence of an awakening response for various salivary biomarkers of adrenocortical activity, including dehydroepiandrosterone-sulphate (DHEA-S), which acts as a cortisol antagonist, and α-amylase, which is a predictor of circulating catecholamine activity. Salivary biological indicators are considered to be valuable markers of hypothalamus-pituitary-adrenal (HPA) axis diurnal activity.

METHODS

In an attempt to overcome problems associated with non-adherence to the requested sampling protocol, only young, healthy males with a physiological CAR value (defined as a 50% increase in salivary cortisol within 30 min after waking) were included in the study (67 out of 102 who initially enrolled met this criterion).

RESULTS

Our results suggested that, as is already known for cortisol, DHEA-S and α-amylase have significant awakening responses. In addition, daily profile of salivary cortisol, α-amylase and DHEA-S fluctuations were analysed. Significant correlations were found between salivary cortisol, DHEA-S and α-amylase levels. The results showed that cortisol and DHEA-S concentrations were inversely correlated with α-amylase levels.

CONCLUSION

This correlation confirmed the distinctiveness of the two regulatory systems: salivary cortisol and DHEA-S concentrations reflect the activity of the HPA axis, whereas α-amylase activity is more closely related to sympathetic activity. In addition, the present study emphasizes the potential value of saliva collection (which is both easy and stress-free) in monitoring changes of adrenal function, confirming that multiple sampling (especially within 1 h after awakening) is necessary to reliably characterise biomarker activity when investigating neuroendocrine changes under various conditions.

Authors+Show Affiliations

Department of Pharmacology, School of Medicine, Gr. T. Popa University of Medicine and Pharmacy, Iasi, Romania.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21876512

Citation

Ghiciuc, Cristina Mihaela, et al. "Awakening Responses and Diurnal Fluctuations of Salivary Cortisol, DHEA-S and Α-amylase in Healthy Male Subjects." Neuro Endocrinology Letters, vol. 32, no. 4, 2011, pp. 475-80.
Ghiciuc CM, Cozma-Dima CL, Pasquali V, et al. Awakening responses and diurnal fluctuations of salivary cortisol, DHEA-S and α-amylase in healthy male subjects. Neuro Endocrinol Lett. 2011;32(4):475-80.
Ghiciuc, C. M., Cozma-Dima, C. L., Pasquali, V., Renzi, P., Simeoni, S., Lupusoru, C. E., & Patacchioli, F. R. (2011). Awakening responses and diurnal fluctuations of salivary cortisol, DHEA-S and α-amylase in healthy male subjects. Neuro Endocrinology Letters, 32(4), 475-80.
Ghiciuc CM, et al. Awakening Responses and Diurnal Fluctuations of Salivary Cortisol, DHEA-S and Α-amylase in Healthy Male Subjects. Neuro Endocrinol Lett. 2011;32(4):475-80. PubMed PMID: 21876512.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Awakening responses and diurnal fluctuations of salivary cortisol, DHEA-S and α-amylase in healthy male subjects. AU - Ghiciuc,Cristina Mihaela, AU - Cozma-Dima,Corina Lucia, AU - Pasquali,Vittorio, AU - Renzi,Paolo, AU - Simeoni,Simona, AU - Lupusoru,Catalina Elena, AU - Patacchioli,Francesca Romana, PY - 2011/05/05/received PY - 2011/07/26/accepted PY - 2011/8/31/entrez PY - 2011/8/31/pubmed PY - 2011/11/1/medline SP - 475 EP - 80 JF - Neuro endocrinology letters JO - Neuro Endocrinol Lett VL - 32 IS - 4 N2 - OBJECTIVES: Because the cortisol awakening response (CAR) has received increasing attention as a useful index of adrenocortical activity, the primary objective of this study was to investigate the presence of an awakening response for various salivary biomarkers of adrenocortical activity, including dehydroepiandrosterone-sulphate (DHEA-S), which acts as a cortisol antagonist, and α-amylase, which is a predictor of circulating catecholamine activity. Salivary biological indicators are considered to be valuable markers of hypothalamus-pituitary-adrenal (HPA) axis diurnal activity. METHODS: In an attempt to overcome problems associated with non-adherence to the requested sampling protocol, only young, healthy males with a physiological CAR value (defined as a 50% increase in salivary cortisol within 30 min after waking) were included in the study (67 out of 102 who initially enrolled met this criterion). RESULTS: Our results suggested that, as is already known for cortisol, DHEA-S and α-amylase have significant awakening responses. In addition, daily profile of salivary cortisol, α-amylase and DHEA-S fluctuations were analysed. Significant correlations were found between salivary cortisol, DHEA-S and α-amylase levels. The results showed that cortisol and DHEA-S concentrations were inversely correlated with α-amylase levels. CONCLUSION: This correlation confirmed the distinctiveness of the two regulatory systems: salivary cortisol and DHEA-S concentrations reflect the activity of the HPA axis, whereas α-amylase activity is more closely related to sympathetic activity. In addition, the present study emphasizes the potential value of saliva collection (which is both easy and stress-free) in monitoring changes of adrenal function, confirming that multiple sampling (especially within 1 h after awakening) is necessary to reliably characterise biomarker activity when investigating neuroendocrine changes under various conditions. SN - 0172-780X UR - https://www.unboundmedicine.com/medline/citation/21876512/Awakening_responses_and_diurnal_fluctuations_of_salivary_cortisol_DHEA_S_and_α_amylase_in_healthy_male_subjects_ L2 - https://antibodies.cancer.gov/detail/CPTC-MET-1 DB - PRIME DP - Unbound Medicine ER -