Tags

Type your tag names separated by a space and hit enter

Indole-containing fractions of Brassica rapa inhibit inducible nitric oxide synthase and pro-inflammatory cytokine expression by inactivating nuclear factor-κB.
J Med Food. 2011 Dec; 14(12):1527-37.JM

Abstract

In an attempt to identify bioactive natural products with anti-inflammatory activity, we evaluated the anti-inflammatory potential of the indole-containing fraction from the roots of Brassica rapa (IBR) (Family Brassicaceae) and the underlying mechanisms. Initially, we examined the inhibitory effect of IBR on the production of pro-inflammatory mediators in vitro and then evaluated its in vivo anti-inflammatory effects. IBR was found to concentration-dependently reduce the productions of nitric oxide, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in lipopolysaccharide (LPS)-induced macrophages. Consistent with these findings, IBR suppressed the LPS-induced expressions of inducible nitric oxide synthase (iNOS) at the protein level and of iNOS, TNF-α, and IL-6 at the mRNA level. Furthermore, IBR attenuated LPS-induced DNA-binding activities of nuclear factor-κB (NF-κB), and this was accompanied by a parallel reduction in the degradation and phosphorylation of inhibitory κBα and, consequently, by a reduction in the nuclear translocation of the p65 subunit of NF-κB. In addition, treatment with IBR inhibited carrageenan-induced paw edema in rats and acetic acid-induced writing response in mice. Taken together, our data suggest that the expressional inhibitions of iNOS, TNF-α, and IL-6 caused by an attenuation of NF-κB activation are responsible for the anti-inflammatory and antinociceptive activity of IBR.

Authors+Show Affiliations

Department of Pharmaceutical Biochemistry, Kyung Hee University, Seoul, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21877949

Citation

Shin, Ji-Sun, et al. "Indole-containing Fractions of Brassica Rapa Inhibit Inducible Nitric Oxide Synthase and Pro-inflammatory Cytokine Expression By Inactivating Nuclear Factor-κB." Journal of Medicinal Food, vol. 14, no. 12, 2011, pp. 1527-37.
Shin JS, Yun CH, Cho YW, et al. Indole-containing fractions of Brassica rapa inhibit inducible nitric oxide synthase and pro-inflammatory cytokine expression by inactivating nuclear factor-κB. J Med Food. 2011;14(12):1527-37.
Shin, J. S., Yun, C. H., Cho, Y. W., Baek, N. I., Choi, M. S., Jeong, T. S., Chung, H. G., & Lee, K. T. (2011). Indole-containing fractions of Brassica rapa inhibit inducible nitric oxide synthase and pro-inflammatory cytokine expression by inactivating nuclear factor-κB. Journal of Medicinal Food, 14(12), 1527-37. https://doi.org/10.1089/jmf.2011.1611
Shin JS, et al. Indole-containing Fractions of Brassica Rapa Inhibit Inducible Nitric Oxide Synthase and Pro-inflammatory Cytokine Expression By Inactivating Nuclear Factor-κB. J Med Food. 2011;14(12):1527-37. PubMed PMID: 21877949.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Indole-containing fractions of Brassica rapa inhibit inducible nitric oxide synthase and pro-inflammatory cytokine expression by inactivating nuclear factor-κB. AU - Shin,Ji-Sun, AU - Yun,Chang Hyeon, AU - Cho,Young-Wuk, AU - Baek,Nam-In, AU - Choi,Myung-Sook, AU - Jeong,Tae-Sook, AU - Chung,Hae-Gon, AU - Lee,Kyung-Tae, Y1 - 2011/08/30/ PY - 2011/9/1/entrez PY - 2011/9/1/pubmed PY - 2012/4/6/medline SP - 1527 EP - 37 JF - Journal of medicinal food JO - J Med Food VL - 14 IS - 12 N2 - In an attempt to identify bioactive natural products with anti-inflammatory activity, we evaluated the anti-inflammatory potential of the indole-containing fraction from the roots of Brassica rapa (IBR) (Family Brassicaceae) and the underlying mechanisms. Initially, we examined the inhibitory effect of IBR on the production of pro-inflammatory mediators in vitro and then evaluated its in vivo anti-inflammatory effects. IBR was found to concentration-dependently reduce the productions of nitric oxide, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in lipopolysaccharide (LPS)-induced macrophages. Consistent with these findings, IBR suppressed the LPS-induced expressions of inducible nitric oxide synthase (iNOS) at the protein level and of iNOS, TNF-α, and IL-6 at the mRNA level. Furthermore, IBR attenuated LPS-induced DNA-binding activities of nuclear factor-κB (NF-κB), and this was accompanied by a parallel reduction in the degradation and phosphorylation of inhibitory κBα and, consequently, by a reduction in the nuclear translocation of the p65 subunit of NF-κB. In addition, treatment with IBR inhibited carrageenan-induced paw edema in rats and acetic acid-induced writing response in mice. Taken together, our data suggest that the expressional inhibitions of iNOS, TNF-α, and IL-6 caused by an attenuation of NF-κB activation are responsible for the anti-inflammatory and antinociceptive activity of IBR. SN - 1557-7600 UR - https://www.unboundmedicine.com/medline/citation/21877949/Indole_containing_fractions_of_Brassica_rapa_inhibit_inducible_nitric_oxide_synthase_and_pro_inflammatory_cytokine_expression_by_inactivating_nuclear_factor_κB_ L2 - https://www.liebertpub.com/doi/10.1089/jmf.2011.1611?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -