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Aptameric system for the highly selective and ultrasensitive detection of protein in human serum based on non-stripping gold nanoparticles.
Analyst. 2011 Oct 21; 136(20):4144-51.A

Abstract

A novel approach is proposed in this study for the development of an aptameric assay system for protein based on non-stripping gold nanoparticles (NPs)-triggered chemiluminescence (CL) upon target binding. The strategy chiefly depends on the formation of a sandwich-type immunocomplex among the capture antibody immobilized on the polystyrene microwells, target protein and aptamer-functionalized gold NPs. Introduction of target protein into the assay system leads to the attachment of gold NPs onto the surface of the microwells and thus the assembled gold NPs could trigger the reaction between luminol and AgNO(3) with a CL emission. Further signal amplification was achieved by a simple gold metal catalytic deposition onto the gold NPs. Such an amplified CL transduction allowed for the detection of model target IgE down to the 50 fM, which is better than most existing aptameric methods for IgE detection. This new protocol also provided a good capability in discriminating IgE from nontarget proteins such as IgG, IgA, IgM and interferon. The practical application of the proposed gold NPs-based immunoassay was successfully carried out for the determination of IgE in 35 human serum samples. Overall, the proposed assay system exhibits excellent analytical characteristics (e.g., a detection limit on the attomolar scale and a linear dynamic range of 4 orders of magnitude), and it is also straightforward to adapt this strategy to detect a spectrum of other proteins by using different aptamers. This new CL strategy might create a novel technology for developing simple biosensors in the sensitive and selective detection of target protein in a variety of clinical, environmental and biodefense applications.

Authors+Show Affiliations

School of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education & PLA, Fudan University, 826 Zhangheng Road, Shanghai, 201203, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21881666

Citation

Sun, Yanhua, et al. "Aptameric System for the Highly Selective and Ultrasensitive Detection of Protein in Human Serum Based On Non-stripping Gold Nanoparticles." The Analyst, vol. 136, no. 20, 2011, pp. 4144-51.
Sun Y, Cai S, Cao Z, et al. Aptameric system for the highly selective and ultrasensitive detection of protein in human serum based on non-stripping gold nanoparticles. Analyst. 2011;136(20):4144-51.
Sun, Y., Cai, S., Cao, Z., Lau, C., & Lu, J. (2011). Aptameric system for the highly selective and ultrasensitive detection of protein in human serum based on non-stripping gold nanoparticles. The Analyst, 136(20), 4144-51. https://doi.org/10.1039/c1an15520b
Sun Y, et al. Aptameric System for the Highly Selective and Ultrasensitive Detection of Protein in Human Serum Based On Non-stripping Gold Nanoparticles. Analyst. 2011 Oct 21;136(20):4144-51. PubMed PMID: 21881666.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aptameric system for the highly selective and ultrasensitive detection of protein in human serum based on non-stripping gold nanoparticles. AU - Sun,Yanhua, AU - Cai,Sheng, AU - Cao,Zhijuan, AU - Lau,Choiwan, AU - Lu,Jianzhong, Y1 - 2011/09/01/ PY - 2011/9/2/entrez PY - 2011/9/2/pubmed PY - 2012/2/1/medline SP - 4144 EP - 51 JF - The Analyst JO - Analyst VL - 136 IS - 20 N2 - A novel approach is proposed in this study for the development of an aptameric assay system for protein based on non-stripping gold nanoparticles (NPs)-triggered chemiluminescence (CL) upon target binding. The strategy chiefly depends on the formation of a sandwich-type immunocomplex among the capture antibody immobilized on the polystyrene microwells, target protein and aptamer-functionalized gold NPs. Introduction of target protein into the assay system leads to the attachment of gold NPs onto the surface of the microwells and thus the assembled gold NPs could trigger the reaction between luminol and AgNO(3) with a CL emission. Further signal amplification was achieved by a simple gold metal catalytic deposition onto the gold NPs. Such an amplified CL transduction allowed for the detection of model target IgE down to the 50 fM, which is better than most existing aptameric methods for IgE detection. This new protocol also provided a good capability in discriminating IgE from nontarget proteins such as IgG, IgA, IgM and interferon. The practical application of the proposed gold NPs-based immunoassay was successfully carried out for the determination of IgE in 35 human serum samples. Overall, the proposed assay system exhibits excellent analytical characteristics (e.g., a detection limit on the attomolar scale and a linear dynamic range of 4 orders of magnitude), and it is also straightforward to adapt this strategy to detect a spectrum of other proteins by using different aptamers. This new CL strategy might create a novel technology for developing simple biosensors in the sensitive and selective detection of target protein in a variety of clinical, environmental and biodefense applications. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/21881666/Aptameric_system_for_the_highly_selective_and_ultrasensitive_detection_of_protein_in_human_serum_based_on_non_stripping_gold_nanoparticles_ L2 - https://doi.org/10.1039/c1an15520b DB - PRIME DP - Unbound Medicine ER -