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Activation of transient receptor potential A1 by a non-pungent capsaicin-like compound, capsiate.
Br J Pharmacol. 2012 Mar; 165(5):1476-86.BJ

Abstract

BACKGROUND AND PURPOSE

Capsiate is produced by 'CH-19 Sweet' (Capsicum annuun L.), a non-pungent cultivar of red pepper. Like capsaicin, capsiate is thought to enhance energy metabolism by activating the sympathetic nervous system and suppressing inflammation, but the underlying mechanisms for this are uncertain. We previously reported that capsiate could activate transient receptor potential vanilloid 1 (TRPV1), a capsaicin receptor. The purpose of the present study is to investigate whether capsinoids activate other TRP channels.

EXPERIMENTAL APPROACH

Using Ca(2+) imaging and whole-cell patch-clamp methods, we analysed the response of TRP channels to three kinds of capsinoids, capsiate, dihydrocapsiate and nordihydrocapsiate, in HEK293T cells expressing TRP channels or in primary cultures of mouse dorsal root ganglion neurons.

KEY RESULTS

We found that in both cell types TRP ankyrin 1 (TRPA1) had a slightly weaker response to capsinoids compared with TRPV1, with the capsiate EC(50) for TRPA1 activation being more than that for TRPV1 activation, and that the capsinoid-evoked action was blocked by a specific TRPA1 antagonist. TRPA1 was activated by capsinoids, but not by their degradation products. Amino acids known to participate in TRPA1 activation following cysteine covalent modification or zinc treatment were not involved in the activation of TRPA1 by capsinoid.

CONCLUSIONS AND IMPLICATIONS

Taken together, these results indicate that capsinoids activate TRPA1 by an as yet unknown mechanism, and TRPA1 could be involved in physiological phenomena associated with capsinoid treatment.

Authors+Show Affiliations

Division of Cell Signaling, Okazaki Institute for Integrative Bioscience (National Institute for Physiological Sciences), National Institutes of Natural Sciences, Okazaki, Aichi, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21883144

Citation

Shintaku, Kenji, et al. "Activation of Transient Receptor Potential A1 By a Non-pungent Capsaicin-like Compound, Capsiate." British Journal of Pharmacology, vol. 165, no. 5, 2012, pp. 1476-86.
Shintaku K, Uchida K, Suzuki Y, et al. Activation of transient receptor potential A1 by a non-pungent capsaicin-like compound, capsiate. Br J Pharmacol. 2012;165(5):1476-86.
Shintaku, K., Uchida, K., Suzuki, Y., Zhou, Y., Fushiki, T., Watanabe, T., Yazawa, S., & Tominaga, M. (2012). Activation of transient receptor potential A1 by a non-pungent capsaicin-like compound, capsiate. British Journal of Pharmacology, 165(5), 1476-86. https://doi.org/10.1111/j.1476-5381.2011.01634.x
Shintaku K, et al. Activation of Transient Receptor Potential A1 By a Non-pungent Capsaicin-like Compound, Capsiate. Br J Pharmacol. 2012;165(5):1476-86. PubMed PMID: 21883144.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activation of transient receptor potential A1 by a non-pungent capsaicin-like compound, capsiate. AU - Shintaku,Kenji, AU - Uchida,Kunitoshi, AU - Suzuki,Yoshiro, AU - Zhou,Yiming, AU - Fushiki,Tohru, AU - Watanabe,Tatsuo, AU - Yazawa,Susumu, AU - Tominaga,Makoto, PY - 2011/9/3/entrez PY - 2011/9/3/pubmed PY - 2012/9/8/medline SP - 1476 EP - 86 JF - British journal of pharmacology JO - Br J Pharmacol VL - 165 IS - 5 N2 - BACKGROUND AND PURPOSE: Capsiate is produced by 'CH-19 Sweet' (Capsicum annuun L.), a non-pungent cultivar of red pepper. Like capsaicin, capsiate is thought to enhance energy metabolism by activating the sympathetic nervous system and suppressing inflammation, but the underlying mechanisms for this are uncertain. We previously reported that capsiate could activate transient receptor potential vanilloid 1 (TRPV1), a capsaicin receptor. The purpose of the present study is to investigate whether capsinoids activate other TRP channels. EXPERIMENTAL APPROACH: Using Ca(2+) imaging and whole-cell patch-clamp methods, we analysed the response of TRP channels to three kinds of capsinoids, capsiate, dihydrocapsiate and nordihydrocapsiate, in HEK293T cells expressing TRP channels or in primary cultures of mouse dorsal root ganglion neurons. KEY RESULTS: We found that in both cell types TRP ankyrin 1 (TRPA1) had a slightly weaker response to capsinoids compared with TRPV1, with the capsiate EC(50) for TRPA1 activation being more than that for TRPV1 activation, and that the capsinoid-evoked action was blocked by a specific TRPA1 antagonist. TRPA1 was activated by capsinoids, but not by their degradation products. Amino acids known to participate in TRPA1 activation following cysteine covalent modification or zinc treatment were not involved in the activation of TRPA1 by capsinoid. CONCLUSIONS AND IMPLICATIONS: Taken together, these results indicate that capsinoids activate TRPA1 by an as yet unknown mechanism, and TRPA1 could be involved in physiological phenomena associated with capsinoid treatment. SN - 1476-5381 UR - https://www.unboundmedicine.com/medline/citation/21883144/Activation_of_transient_receptor_potential_A1_by_a_non_pungent_capsaicin_like_compound_capsiate_ L2 - https://doi.org/10.1111/j.1476-5381.2011.01634.x DB - PRIME DP - Unbound Medicine ER -