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Genesis of muscle fiber-type diversity during mouse embryogenesis relies on Six1 and Six4 gene expression.
Dev Biol. 2011 Nov 15; 359(2):303-20.DB

Abstract

Adult skeletal muscles in vertebrates are composed of different types of myofibers endowed with distinct metabolic and contraction speed properties. Genesis of this fiber-type heterogeneity during development remains poorly known, at least in mammals. Six1 and Six4 homeoproteins of the Six/sine oculis family are expressed throughout muscle development in mice, and Six1 protein is enriched in the nuclei of adult fast-twitch myofibers. Furthermore, Six1/Six4 proteins are known to control the early activation of fast-type muscle genes in myocytes present in the mouse somitic myotome. Using double Six1:Six4 mutants (SixdKO) to dissect in vivo the genesis of muscle fiber-type heterogeneity, we analyzed here the phenotype of the dorsal/epaxial muscles remaining in SixdKO. We show by electron microscopy analysis that the absence of these homeoproteins precludes normal sarcomeric organization of the myofiber leading to a dystrophic aspect, and by immunohistochemistry experiments a deficiency in synaptogenesis. Affymetrix transcriptome analysis of the muscles remaining in E18.5 SixdKO identifies a major role for these homeoproteins in the control of genes that are specifically activated in the adult fast/glycolytic myofibers, particularly those controlling Ca(2+) homeostasis. Absence of Six1 and Six4 leads to the development of dorsal myofibers lacking expression of fast-type muscle genes, and mainly expressing a slow-type muscle program. The absence of restriction of the slow-type program during the fetal period in SixdKO back muscles is associated with a decreased HDAC4 protein level, and subcellular relocalization of the transcription repressor Sox6. Six genes thus behave as essential global regulators of muscle gene expression, as well as a central switch to drive the skeletal muscle fast phenotype during fetal development.

Authors+Show Affiliations

Institut Cochin, Université Paris Descartes, Sorbonne Paris Cité, CNRS (UMR 8104), Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

21884692

Citation

Richard, Anne-Françoise, et al. "Genesis of Muscle Fiber-type Diversity During Mouse Embryogenesis Relies On Six1 and Six4 Gene Expression." Developmental Biology, vol. 359, no. 2, 2011, pp. 303-20.
Richard AF, Demignon J, Sakakibara I, et al. Genesis of muscle fiber-type diversity during mouse embryogenesis relies on Six1 and Six4 gene expression. Dev Biol. 2011;359(2):303-20.
Richard, A. F., Demignon, J., Sakakibara, I., Pujol, J., Favier, M., Strochlic, L., Le Grand, F., Sgarioto, N., Guernec, A., Schmitt, A., Cagnard, N., Huang, R., Legay, C., Guillet-Deniau, I., & Maire, P. (2011). Genesis of muscle fiber-type diversity during mouse embryogenesis relies on Six1 and Six4 gene expression. Developmental Biology, 359(2), 303-20. https://doi.org/10.1016/j.ydbio.2011.08.010
Richard AF, et al. Genesis of Muscle Fiber-type Diversity During Mouse Embryogenesis Relies On Six1 and Six4 Gene Expression. Dev Biol. 2011 Nov 15;359(2):303-20. PubMed PMID: 21884692.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genesis of muscle fiber-type diversity during mouse embryogenesis relies on Six1 and Six4 gene expression. AU - Richard,Anne-Françoise, AU - Demignon,Josiane, AU - Sakakibara,Iori, AU - Pujol,Julien, AU - Favier,Maryline, AU - Strochlic,Laure, AU - Le Grand,Fabien, AU - Sgarioto,Nicolas, AU - Guernec,Anthony, AU - Schmitt,Alain, AU - Cagnard,Nicolas, AU - Huang,Ruijin, AU - Legay,Claire, AU - Guillet-Deniau,Isabelle, AU - Maire,Pascal, Y1 - 2011/08/22/ PY - 2011/03/23/received PY - 2011/07/22/revised PY - 2011/08/15/accepted PY - 2011/9/3/entrez PY - 2011/9/3/pubmed PY - 2012/1/27/medline SP - 303 EP - 20 JF - Developmental biology JO - Dev. Biol. VL - 359 IS - 2 N2 - Adult skeletal muscles in vertebrates are composed of different types of myofibers endowed with distinct metabolic and contraction speed properties. Genesis of this fiber-type heterogeneity during development remains poorly known, at least in mammals. Six1 and Six4 homeoproteins of the Six/sine oculis family are expressed throughout muscle development in mice, and Six1 protein is enriched in the nuclei of adult fast-twitch myofibers. Furthermore, Six1/Six4 proteins are known to control the early activation of fast-type muscle genes in myocytes present in the mouse somitic myotome. Using double Six1:Six4 mutants (SixdKO) to dissect in vivo the genesis of muscle fiber-type heterogeneity, we analyzed here the phenotype of the dorsal/epaxial muscles remaining in SixdKO. We show by electron microscopy analysis that the absence of these homeoproteins precludes normal sarcomeric organization of the myofiber leading to a dystrophic aspect, and by immunohistochemistry experiments a deficiency in synaptogenesis. Affymetrix transcriptome analysis of the muscles remaining in E18.5 SixdKO identifies a major role for these homeoproteins in the control of genes that are specifically activated in the adult fast/glycolytic myofibers, particularly those controlling Ca(2+) homeostasis. Absence of Six1 and Six4 leads to the development of dorsal myofibers lacking expression of fast-type muscle genes, and mainly expressing a slow-type muscle program. The absence of restriction of the slow-type program during the fetal period in SixdKO back muscles is associated with a decreased HDAC4 protein level, and subcellular relocalization of the transcription repressor Sox6. Six genes thus behave as essential global regulators of muscle gene expression, as well as a central switch to drive the skeletal muscle fast phenotype during fetal development. SN - 1095-564X UR - https://www.unboundmedicine.com/medline/citation/21884692/Genesis_of_muscle_fiber_type_diversity_during_mouse_embryogenesis_relies_on_Six1_and_Six4_gene_expression_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012-1606(11)01197-3 DB - PRIME DP - Unbound Medicine ER -