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In vitro metabolism in Sprague-Dawley rat liver microsomes of forsythoside A in different compositions of Shuang-Huang-Lian.
Fitoterapia. 2011 Dec; 82(8):1222-30.F

Abstract

Shuang-Huang-Lian (SHL), a traditional Chinese formula containing Lonicerae japonicae flos (LJF), Scutellariae radix (SR) and Forsythiae fructus (FF), is commonly used to treat acute upper respiratory tract infection, acute bronchitis and light pneumonia. Forsythoside A is one of the main active ingredients in Forsythiae fructus, a key herb in SHL. In the present study, effects of different compositions in SHL on the in vitro metabolism in Sprague-Dawley rat liver microsomes of forsythoside A were investigated. The observations from Sprague-Dawley rat liver microsomes in the presence of β-NADPH or UDPGA that forsythoside A may be the substrates of CYP3A4, CYP2C9, CYP1A2, UGT1A6, UGT1A3, UGT1A1 and UGT1A9; Chlorogenic acid may be the substrates of CYP3A4, CYP2C9, CYP1A2, CYP2C19, UGT1A6, UGT1A3 and UGT1A1; Baicalin may be the substrates of CYP3A4, CYP2C19, CYP1A2, UGT1A9, UGT1A1 and UGT1A3; Baicalein may be the substrates of CYP3A4, CYP2E1 and UGT1A6. It was also found that the residue of forsythoside A in SHL, FF+LJF and FF+SR was greatly increased compared with that in FF in Sprague-Dawley rat liver microsomes in the presence of β-NADPH or UDPGA, which indicated that the metabolism of forsythoside A in SHL may be influenced by chlorogenic acid in LJF acting on the CYP3A4, CYP2C9, CYP1A2, UGT1A6, UGT1A3 and UGT1A1; baicalin in SR acting on the CYP3A4, CYP1A2, UGT1A9, UGT1A1 and UGT1A3; baicalein acting on the CYP3A4 and UGT1A6 respectively.

Authors+Show Affiliations

College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21888954

Citation

Zhou, Wei, et al. "In Vitro Metabolism in Sprague-Dawley Rat Liver Microsomes of Forsythoside a in Different Compositions of Shuang-Huang-Lian." Fitoterapia, vol. 82, no. 8, 2011, pp. 1222-30.
Zhou W, Di LQ, Shan JJ, et al. In vitro metabolism in Sprague-Dawley rat liver microsomes of forsythoside A in different compositions of Shuang-Huang-Lian. Fitoterapia. 2011;82(8):1222-30.
Zhou, W., Di, L. Q., Shan, J. J., Bi, X. L., Chen, L. T., Wang, L. C., & Cai, B. C. (2011). In vitro metabolism in Sprague-Dawley rat liver microsomes of forsythoside A in different compositions of Shuang-Huang-Lian. Fitoterapia, 82(8), 1222-30. https://doi.org/10.1016/j.fitote.2011.08.009
Zhou W, et al. In Vitro Metabolism in Sprague-Dawley Rat Liver Microsomes of Forsythoside a in Different Compositions of Shuang-Huang-Lian. Fitoterapia. 2011;82(8):1222-30. PubMed PMID: 21888954.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro metabolism in Sprague-Dawley rat liver microsomes of forsythoside A in different compositions of Shuang-Huang-Lian. AU - Zhou,Wei, AU - Di,Liu-qing, AU - Shan,Jin-jun, AU - Bi,Xiao-lin, AU - Chen,Le-tian, AU - Wang,Ling-chong, AU - Cai,Bao-chang, Y1 - 2011/08/23/ PY - 2011/06/22/received PY - 2011/08/11/revised PY - 2011/08/14/accepted PY - 2011/9/6/entrez PY - 2011/9/6/pubmed PY - 2012/4/26/medline SP - 1222 EP - 30 JF - Fitoterapia JO - Fitoterapia VL - 82 IS - 8 N2 - Shuang-Huang-Lian (SHL), a traditional Chinese formula containing Lonicerae japonicae flos (LJF), Scutellariae radix (SR) and Forsythiae fructus (FF), is commonly used to treat acute upper respiratory tract infection, acute bronchitis and light pneumonia. Forsythoside A is one of the main active ingredients in Forsythiae fructus, a key herb in SHL. In the present study, effects of different compositions in SHL on the in vitro metabolism in Sprague-Dawley rat liver microsomes of forsythoside A were investigated. The observations from Sprague-Dawley rat liver microsomes in the presence of β-NADPH or UDPGA that forsythoside A may be the substrates of CYP3A4, CYP2C9, CYP1A2, UGT1A6, UGT1A3, UGT1A1 and UGT1A9; Chlorogenic acid may be the substrates of CYP3A4, CYP2C9, CYP1A2, CYP2C19, UGT1A6, UGT1A3 and UGT1A1; Baicalin may be the substrates of CYP3A4, CYP2C19, CYP1A2, UGT1A9, UGT1A1 and UGT1A3; Baicalein may be the substrates of CYP3A4, CYP2E1 and UGT1A6. It was also found that the residue of forsythoside A in SHL, FF+LJF and FF+SR was greatly increased compared with that in FF in Sprague-Dawley rat liver microsomes in the presence of β-NADPH or UDPGA, which indicated that the metabolism of forsythoside A in SHL may be influenced by chlorogenic acid in LJF acting on the CYP3A4, CYP2C9, CYP1A2, UGT1A6, UGT1A3 and UGT1A1; baicalin in SR acting on the CYP3A4, CYP1A2, UGT1A9, UGT1A1 and UGT1A3; baicalein acting on the CYP3A4 and UGT1A6 respectively. SN - 1873-6971 UR - https://www.unboundmedicine.com/medline/citation/21888954/In_vitro_metabolism_in_Sprague_Dawley_rat_liver_microsomes_of_forsythoside_A_in_different_compositions_of_Shuang_Huang_Lian_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0367-326X(11)00207-3 DB - PRIME DP - Unbound Medicine ER -