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Induction of retinal pigment epithelial cells from monkey iPS cells.
Invest Ophthalmol Vis Sci. 2011 Nov 11; 52(12):8785-90.IO

Abstract

PURPOSE

The induced pluripotent stem (iPS) cell is expected to be a powerful tool for research and development in regenerative medicine. Previously, the authors reported that human iPS cells differentiated into retinal cells, including photoreceptors and retinal pigment epithelial cells. In this study, they produced iPS cell lines from monkeys to investigate their ability to differentiate into retinal cells.

METHODS

To generate iPS cells, the fibroblasts derived from cynomolgus monkey abdominal skin were infected with retroviruses carrying Oct3/4, Sox2, Klf4, and c-Myc genes and then were cultured on STO feeder cells. Next, the established iPS cells were cultured with the conditioned medium of PA6 cells to induce RPE cells. The properties of the differentiated RPE cells were analyzed.

RESULTS

Approximately 1 month after viral infection, some epithelial-like colonies appeared among the fibroblasts. These colonies were morphologically similar to the cynomolgus embryonic stem (ES) cell and expressed ES cell-specific markers. By producing teratomas in SCID mice, these cells were confirmed to have the ability to differentiate into three germ layers. In addition, the RPE cells induced from the monkey iPS cells had characteristic polygonal shapes and pigments. These cells expressed RPE cell-specific markers such as RPE65, CRALBP, Bestrophin 1, and MERTK and exhibited phagocytotic function in vitro.

CONCLUSIONS

The RPE cells derived from monkey skin with iPS cell technology can be used for autologous or allogeneic transplantation to test the possibility of immune rejection and to evaluate their function in vivo with the same techniques that will be used in clinical trials.

Authors+Show Affiliations

Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe, Japan.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21896853

Citation

Okamoto, Satoshi, and Masayo Takahashi. "Induction of Retinal Pigment Epithelial Cells From Monkey iPS Cells." Investigative Ophthalmology & Visual Science, vol. 52, no. 12, 2011, pp. 8785-90.
Okamoto S, Takahashi M. Induction of retinal pigment epithelial cells from monkey iPS cells. Invest Ophthalmol Vis Sci. 2011;52(12):8785-90.
Okamoto, S., & Takahashi, M. (2011). Induction of retinal pigment epithelial cells from monkey iPS cells. Investigative Ophthalmology & Visual Science, 52(12), 8785-90. https://doi.org/10.1167/iovs.11-8129
Okamoto S, Takahashi M. Induction of Retinal Pigment Epithelial Cells From Monkey iPS Cells. Invest Ophthalmol Vis Sci. 2011 Nov 11;52(12):8785-90. PubMed PMID: 21896853.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of retinal pigment epithelial cells from monkey iPS cells. AU - Okamoto,Satoshi, AU - Takahashi,Masayo, Y1 - 2011/11/11/ PY - 2011/9/8/entrez PY - 2011/9/8/pubmed PY - 2012/1/6/medline SP - 8785 EP - 90 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 52 IS - 12 N2 - PURPOSE: The induced pluripotent stem (iPS) cell is expected to be a powerful tool for research and development in regenerative medicine. Previously, the authors reported that human iPS cells differentiated into retinal cells, including photoreceptors and retinal pigment epithelial cells. In this study, they produced iPS cell lines from monkeys to investigate their ability to differentiate into retinal cells. METHODS: To generate iPS cells, the fibroblasts derived from cynomolgus monkey abdominal skin were infected with retroviruses carrying Oct3/4, Sox2, Klf4, and c-Myc genes and then were cultured on STO feeder cells. Next, the established iPS cells were cultured with the conditioned medium of PA6 cells to induce RPE cells. The properties of the differentiated RPE cells were analyzed. RESULTS: Approximately 1 month after viral infection, some epithelial-like colonies appeared among the fibroblasts. These colonies were morphologically similar to the cynomolgus embryonic stem (ES) cell and expressed ES cell-specific markers. By producing teratomas in SCID mice, these cells were confirmed to have the ability to differentiate into three germ layers. In addition, the RPE cells induced from the monkey iPS cells had characteristic polygonal shapes and pigments. These cells expressed RPE cell-specific markers such as RPE65, CRALBP, Bestrophin 1, and MERTK and exhibited phagocytotic function in vitro. CONCLUSIONS: The RPE cells derived from monkey skin with iPS cell technology can be used for autologous or allogeneic transplantation to test the possibility of immune rejection and to evaluate their function in vivo with the same techniques that will be used in clinical trials. SN - 1552-5783 UR - https://www.unboundmedicine.com/medline/citation/21896853/Induction_of_retinal_pigment_epithelial_cells_from_monkey_iPS_cells_ L2 - https://iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.11-8129 DB - PRIME DP - Unbound Medicine ER -