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Sensitization prevalence, antibody cross-reactivity and immunogenic peptide profile of Api g 2, the non-specific lipid transfer protein 1 of celery.
PLoS One 2011; 6(8):e24150Plos

Abstract

BACKGROUND

Celery (Apium graveolens) represents a relevant allergen source that can elicit severe reactions in the adult population. To investigate the sensitization prevalence and cross-reactivity of Api g 2 from celery stalks in a Mediterranean population and in a mouse model.

METHODOLOGY

786 non-randomized subjects from Italy were screened for IgE reactivity to rApi g 2, rArt v 3 (mugwort pollen LTP) and nPru p 3 (peach LTP) using an allergen microarray. Clinical data of 32 selected patients with reactivity to LTP under investigation were evaluated. Specific IgE titers and cross-inhibitions were performed in ELISA and allergen microarray. Balb/c mice were immunized with purified LTPs; IgG titers were determined in ELISA and mediator release was examined using RBL-2H3 cells. Simulated endolysosomal digestion was performed using microsomes obtained from human DCs.

RESULTS

IgE testing showed a sensitization prevalence of 25.6% to Api g 2, 18.6% to Art v 3, and 28.6% to Pru p 3 and frequent co-sensitization and correlating IgE-reactivity was observed. 10/32 patients suffering from LTP-related allergy reported symptoms upon consumption of celery stalks which mainly presented as OAS. Considerable IgE cross-reactivity was observed between Api g 2, Art v 3, and Pru p 3 with varying inhibition degrees of individual patients' sera. Simulating LTP mono-sensitization in a mouse model showed development of more congruent antibody specificities between Api g 2 and Art v 3. Notably, biologically relevant murine IgE cross-reactivity was restricted to the latter and diverse from Pru p 3 epitopes. Endolysosomal processing of LTP showed generation of similar clusters, which presumably represent T-cell peptides.

CONCLUSIONS

Api g 2 represents a relevant celery stalk allergen in the LTP-sensitized population. The molecule displays common B cell epitopes and endolysosomal peptides that encompass T cell epitopes with pollen and plant-food derived LTP.

Authors+Show Affiliations

Christian Doppler Laboratory for Allergy Diagnosis and Therapy, University of Salzburg, Salzburg, Austria. Gabriele.Gadermaier@sbg.ac.atNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21897872

Citation

Gadermaier, Gabriele, et al. "Sensitization Prevalence, Antibody Cross-reactivity and Immunogenic Peptide Profile of Api G 2, the Non-specific Lipid Transfer Protein 1 of Celery." PloS One, vol. 6, no. 8, 2011, pp. e24150.
Gadermaier G, Hauser M, Egger M, et al. Sensitization prevalence, antibody cross-reactivity and immunogenic peptide profile of Api g 2, the non-specific lipid transfer protein 1 of celery. PLoS ONE. 2011;6(8):e24150.
Gadermaier, G., Hauser, M., Egger, M., Ferrara, R., Briza, P., Santos, K. S., ... Ferreira, F. (2011). Sensitization prevalence, antibody cross-reactivity and immunogenic peptide profile of Api g 2, the non-specific lipid transfer protein 1 of celery. PloS One, 6(8), pp. e24150. doi:10.1371/journal.pone.0024150.
Gadermaier G, et al. Sensitization Prevalence, Antibody Cross-reactivity and Immunogenic Peptide Profile of Api G 2, the Non-specific Lipid Transfer Protein 1 of Celery. PLoS ONE. 2011;6(8):e24150. PubMed PMID: 21897872.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitization prevalence, antibody cross-reactivity and immunogenic peptide profile of Api g 2, the non-specific lipid transfer protein 1 of celery. AU - Gadermaier,Gabriele, AU - Hauser,Michael, AU - Egger,Matthias, AU - Ferrara,Rosetta, AU - Briza,Peter, AU - Santos,Keity Souza, AU - Zennaro,Danila, AU - Girbl,Tamara, AU - Zuidmeer-Jongejan,Laurian, AU - Mari,Adriano, AU - Ferreira,Fatima, Y1 - 2011/08/29/ PY - 2011/05/24/received PY - 2011/08/01/accepted PY - 2011/9/8/entrez PY - 2011/9/8/pubmed PY - 2011/12/23/medline SP - e24150 EP - e24150 JF - PloS one JO - PLoS ONE VL - 6 IS - 8 N2 - BACKGROUND: Celery (Apium graveolens) represents a relevant allergen source that can elicit severe reactions in the adult population. To investigate the sensitization prevalence and cross-reactivity of Api g 2 from celery stalks in a Mediterranean population and in a mouse model. METHODOLOGY: 786 non-randomized subjects from Italy were screened for IgE reactivity to rApi g 2, rArt v 3 (mugwort pollen LTP) and nPru p 3 (peach LTP) using an allergen microarray. Clinical data of 32 selected patients with reactivity to LTP under investigation were evaluated. Specific IgE titers and cross-inhibitions were performed in ELISA and allergen microarray. Balb/c mice were immunized with purified LTPs; IgG titers were determined in ELISA and mediator release was examined using RBL-2H3 cells. Simulated endolysosomal digestion was performed using microsomes obtained from human DCs. RESULTS: IgE testing showed a sensitization prevalence of 25.6% to Api g 2, 18.6% to Art v 3, and 28.6% to Pru p 3 and frequent co-sensitization and correlating IgE-reactivity was observed. 10/32 patients suffering from LTP-related allergy reported symptoms upon consumption of celery stalks which mainly presented as OAS. Considerable IgE cross-reactivity was observed between Api g 2, Art v 3, and Pru p 3 with varying inhibition degrees of individual patients' sera. Simulating LTP mono-sensitization in a mouse model showed development of more congruent antibody specificities between Api g 2 and Art v 3. Notably, biologically relevant murine IgE cross-reactivity was restricted to the latter and diverse from Pru p 3 epitopes. Endolysosomal processing of LTP showed generation of similar clusters, which presumably represent T-cell peptides. CONCLUSIONS: Api g 2 represents a relevant celery stalk allergen in the LTP-sensitized population. The molecule displays common B cell epitopes and endolysosomal peptides that encompass T cell epitopes with pollen and plant-food derived LTP. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/21897872/Sensitization_prevalence_antibody_cross_reactivity_and_immunogenic_peptide_profile_of_Api_g_2_the_non_specific_lipid_transfer_protein_1_of_celery_ L2 - http://dx.plos.org/10.1371/journal.pone.0024150 DB - PRIME DP - Unbound Medicine ER -