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Cyclin D3 promotes myogenic differentiation and Pax7 transcription.
J Cell Biochem. 2012 Jan; 113(1):209-19.JC

Abstract

Differentiation of skeletal muscle myoblasts involves activation of muscle-specific markers such as MyoD, Myf5, MRF4, and myogenin, followed by exit from the cell cycle, expression of structural proteins, and fusion into multinucleated myotubes. Cyclin D3 is upregulated during muscle differentiation, and expression of cyclin D3 in proliferating myoblasts causes early activation of myogenesis. In this study, we have identified the genes activated by cyclin D3 expression in C2C12 myoblasts and differentiated cells by real-time PCR analysis. Cyclin D3 expression induced faster differentiation kinetics and increase in levels of myogenic genes such as MyoD, Myf5, and myogenin at an early stage during the differentiation process, although long-term myogenic differentiation was not affected. Transcript levels of the transcription factor Pax7 that is expressed in muscle progenitors were enhanced by cyclin D3 expression in myoblasts. Components of a histone methyltransferase complex recruited by Pax7 to myogenic gene promoters were also regulated by cyclin D3. Further, the Pax7 promoter was upregulated in myoblasts expressing cyclin D3. Myoblasts that expressed cyclin D3 showed moderately higher levels of the cyclin-dependent kinase inhibitor p21 and were stalled in G2/M phase of the cell cycle. Our findings suggest that cyclin D3 primes myoblasts for differentiation by enhancing muscle specific gene expression and cell cycle exit.

Authors+Show Affiliations

Centre for Cellular and Molecular Biology, Hyderabad, India.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21898542

Citation

Gurung, Ritika, and Veena K. Parnaik. "Cyclin D3 Promotes Myogenic Differentiation and Pax7 Transcription." Journal of Cellular Biochemistry, vol. 113, no. 1, 2012, pp. 209-19.
Gurung R, Parnaik VK. Cyclin D3 promotes myogenic differentiation and Pax7 transcription. J Cell Biochem. 2012;113(1):209-19.
Gurung, R., & Parnaik, V. K. (2012). Cyclin D3 promotes myogenic differentiation and Pax7 transcription. Journal of Cellular Biochemistry, 113(1), 209-19. https://doi.org/10.1002/jcb.23346
Gurung R, Parnaik VK. Cyclin D3 Promotes Myogenic Differentiation and Pax7 Transcription. J Cell Biochem. 2012;113(1):209-19. PubMed PMID: 21898542.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cyclin D3 promotes myogenic differentiation and Pax7 transcription. AU - Gurung,Ritika, AU - Parnaik,Veena K, PY - 2011/9/8/entrez PY - 2011/9/8/pubmed PY - 2012/6/16/medline SP - 209 EP - 19 JF - Journal of cellular biochemistry JO - J Cell Biochem VL - 113 IS - 1 N2 - Differentiation of skeletal muscle myoblasts involves activation of muscle-specific markers such as MyoD, Myf5, MRF4, and myogenin, followed by exit from the cell cycle, expression of structural proteins, and fusion into multinucleated myotubes. Cyclin D3 is upregulated during muscle differentiation, and expression of cyclin D3 in proliferating myoblasts causes early activation of myogenesis. In this study, we have identified the genes activated by cyclin D3 expression in C2C12 myoblasts and differentiated cells by real-time PCR analysis. Cyclin D3 expression induced faster differentiation kinetics and increase in levels of myogenic genes such as MyoD, Myf5, and myogenin at an early stage during the differentiation process, although long-term myogenic differentiation was not affected. Transcript levels of the transcription factor Pax7 that is expressed in muscle progenitors were enhanced by cyclin D3 expression in myoblasts. Components of a histone methyltransferase complex recruited by Pax7 to myogenic gene promoters were also regulated by cyclin D3. Further, the Pax7 promoter was upregulated in myoblasts expressing cyclin D3. Myoblasts that expressed cyclin D3 showed moderately higher levels of the cyclin-dependent kinase inhibitor p21 and were stalled in G2/M phase of the cell cycle. Our findings suggest that cyclin D3 primes myoblasts for differentiation by enhancing muscle specific gene expression and cell cycle exit. SN - 1097-4644 UR - https://www.unboundmedicine.com/medline/citation/21898542/Cyclin_D3_promotes_myogenic_differentiation_and_Pax7_transcription_ L2 - https://doi.org/10.1002/jcb.23346 DB - PRIME DP - Unbound Medicine ER -