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[High-yield reassortant virus containing hemagglutinin and neuraminidase genes of pandemic influenza A/Moscowl/01/2009 (H1N1) virus].
Vopr Virusol. 2011 Jul-Aug; 56(4):9-14.VV

Abstract

The crossing of influenza A/Moscow/01/2009 (H1N1) virus and reassortant strain X31 (H3N2) containing the genes of internal and non-structural proteins of A/Puerto Rico/8/34 (H1N1) strain gave rise to reassortant virus ReM8. The reassortant contained hemagglutinin (HA) and neuraminidase (NA) genes of pandemic 2009 influenza virus and 6 genes of high-yield A/Puerto Rico/8/34 (H1N1) strain. The reassortant ReM8 produced higher yields in the embryonated chicken eggs than the parent pandemic virus, as suggested by infectivity and HA activity titration as well as by ELISA and the measurement of HA protein content by scanning electrophoresis in polyacrylamide gel slabs. High immunogenicity of ReM8 reassortant was demonstrated by immune protection studies in mice. The reassortant virus ReM8 is suitable as a candidate strain for the production of inactivated and subunit influenza vaccines.

Authors

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Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

21899062

Citation

Ignat'eva, A V., et al. "[High-yield Reassortant Virus Containing Hemagglutinin and Neuraminidase Genes of Pandemic Influenza A/Moscowl/01/2009 (H1N1) Virus]." Voprosy Virusologii, vol. 56, no. 4, 2011, pp. 9-14.
Ignat'eva AV, Rudneva IA, Timofeeva TA, et al. [High-yield reassortant virus containing hemagglutinin and neuraminidase genes of pandemic influenza A/Moscowl/01/2009 (H1N1) virus]. Vopr Virusol. 2011;56(4):9-14.
Ignat'eva, A. V., Rudneva, I. A., Timofeeva, T. A., Shilov, A. A., Zaberezhnyĭ, A. D., Aliper, T. I., Kaverin, N. V., & L'vov, D. K. (2011). [High-yield reassortant virus containing hemagglutinin and neuraminidase genes of pandemic influenza A/Moscowl/01/2009 (H1N1) virus]. Voprosy Virusologii, 56(4), 9-14.
Ignat'eva AV, et al. [High-yield Reassortant Virus Containing Hemagglutinin and Neuraminidase Genes of Pandemic Influenza A/Moscowl/01/2009 (H1N1) Virus]. Vopr Virusol. 2011 Jul-Aug;56(4):9-14. PubMed PMID: 21899062.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [High-yield reassortant virus containing hemagglutinin and neuraminidase genes of pandemic influenza A/Moscowl/01/2009 (H1N1) virus]. AU - Ignat'eva,A V, AU - Rudneva,I A, AU - Timofeeva,T A, AU - Shilov,A A, AU - Zaberezhnyĭ,A D, AU - Aliper,T I, AU - Kaverin,N V, AU - L'vov,D K, PY - 2011/9/9/entrez PY - 2011/9/9/pubmed PY - 2011/10/1/medline SP - 9 EP - 14 JF - Voprosy virusologii JO - Vopr Virusol VL - 56 IS - 4 N2 - The crossing of influenza A/Moscow/01/2009 (H1N1) virus and reassortant strain X31 (H3N2) containing the genes of internal and non-structural proteins of A/Puerto Rico/8/34 (H1N1) strain gave rise to reassortant virus ReM8. The reassortant contained hemagglutinin (HA) and neuraminidase (NA) genes of pandemic 2009 influenza virus and 6 genes of high-yield A/Puerto Rico/8/34 (H1N1) strain. The reassortant ReM8 produced higher yields in the embryonated chicken eggs than the parent pandemic virus, as suggested by infectivity and HA activity titration as well as by ELISA and the measurement of HA protein content by scanning electrophoresis in polyacrylamide gel slabs. High immunogenicity of ReM8 reassortant was demonstrated by immune protection studies in mice. The reassortant virus ReM8 is suitable as a candidate strain for the production of inactivated and subunit influenza vaccines. SN - 0507-4088 UR - https://www.unboundmedicine.com/medline/citation/21899062/[High_yield_reassortant_virus_containing_hemagglutinin_and_neuraminidase_genes_of_pandemic_influenza_A/Moscowl/01/2009__H1N1__virus]_ L2 - https://medlineplus.gov/flushot.html DB - PRIME DP - Unbound Medicine ER -