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Development and validation of a capillary electrophoresis assay for the determination of the stereoisomeric purity of chloroquine enantiomers.
Electrophoresis. 2011 Oct; 32(19):2669-72.E

Abstract

A stereoselective CE assay for the determination of the enantiomeric purity of (R)-(-)-chloroquine and (S)-(+)-chloroquine was developed and validated. The separations were performed in a 50.2/40 cm uncoated fused silica capillary at 20°C using a 100 mM sodium phosphate buffer, pH 2.5, containing 30 mg/mL sulfobutylether(VII)-β-cyclodextrin as background electrolyte operated at an applied voltage of -25 kV and 20°C. The detection wavelength was 225 nm. Carbamazepine was used as internal standard. The assay was validated in the range of 0.05-1.0% for the respective minor chloroquine enantiomer based on a concentration of 3 mg/mL of the major enantiomer, either (R)-(-)-chloroquine or (S)-(+)-chloroquine. The method was applied to analyze the stereoisomeric purity of synthetic samples of the chloroquine enantiomers.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Wongwan S
Department of Pharmaceutical Chemistry, Friedrich Schiller University Jena, Jena, Germany.
Scriba GK
No affiliation info available

MeSH

ChloroquineDrug ContaminationElectrophoresis, CapillaryLinear ModelsReproducibility of ResultsSensitivity and SpecificityStereoisomerismbeta-Cyclodextrins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21905044

Citation

Wongwan, Sudaporn, and Gerhard K E. Scriba. "Development and Validation of a Capillary Electrophoresis Assay for the Determination of the Stereoisomeric Purity of Chloroquine Enantiomers." Electrophoresis, vol. 32, no. 19, 2011, pp. 2669-72.
Wongwan S, Scriba GK. Development and validation of a capillary electrophoresis assay for the determination of the stereoisomeric purity of chloroquine enantiomers. Electrophoresis. 2011;32(19):2669-72.
Wongwan, S., & Scriba, G. K. (2011). Development and validation of a capillary electrophoresis assay for the determination of the stereoisomeric purity of chloroquine enantiomers. Electrophoresis, 32(19), 2669-72. https://doi.org/10.1002/elps.201000610
Wongwan S, Scriba GK. Development and Validation of a Capillary Electrophoresis Assay for the Determination of the Stereoisomeric Purity of Chloroquine Enantiomers. Electrophoresis. 2011;32(19):2669-72. PubMed PMID: 21905044.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and validation of a capillary electrophoresis assay for the determination of the stereoisomeric purity of chloroquine enantiomers. AU - Wongwan,Sudaporn, AU - Scriba,Gerhard K E, Y1 - 2011/09/08/ PY - 2010/11/11/received PY - 2011/01/11/revised PY - 2011/01/26/accepted PY - 2011/9/10/entrez PY - 2011/9/10/pubmed PY - 2011/12/30/medline SP - 2669 EP - 72 JF - Electrophoresis JO - Electrophoresis VL - 32 IS - 19 N2 - A stereoselective CE assay for the determination of the enantiomeric purity of (R)-(-)-chloroquine and (S)-(+)-chloroquine was developed and validated. The separations were performed in a 50.2/40 cm uncoated fused silica capillary at 20°C using a 100 mM sodium phosphate buffer, pH 2.5, containing 30 mg/mL sulfobutylether(VII)-β-cyclodextrin as background electrolyte operated at an applied voltage of -25 kV and 20°C. The detection wavelength was 225 nm. Carbamazepine was used as internal standard. The assay was validated in the range of 0.05-1.0% for the respective minor chloroquine enantiomer based on a concentration of 3 mg/mL of the major enantiomer, either (R)-(-)-chloroquine or (S)-(+)-chloroquine. The method was applied to analyze the stereoisomeric purity of synthetic samples of the chloroquine enantiomers. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/21905044/Development_and_validation_of_a_capillary_electrophoresis_assay_for_the_determination_of_the_stereoisomeric_purity_of_chloroquine_enantiomers_ L2 - https://doi.org/10.1002/elps.201000610 DB - PRIME DP - Unbound Medicine ER -