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Subcellular interactions of dietary cadmium, copper and zinc in rainbow trout (Oncorhynchus mykiss).
Aquat Toxicol. 2011 Oct; 105(3-4):518-27.AT

Abstract

Interactions of Cu, Cd and Zn were studied at the subcellular level in juvenile rainbow trout (Oncorhynchus mykiss) fed diets containing (μg/g) 500 Cu, 1000 Zn and 500 Cd singly and as a ternary mixture for 28 days. Livers were harvested and submitted to differential centrifugation to isolate components of metabolically active metal pool (MAP: heat-denaturable proteins (HDP), organelles, nuclei) and metabolically detoxified metal pool (MDP: heat stable proteins (HSP), NaOH-resistant granules). Results indicated that Cd accumulation was enhanced in all the subcellular compartments, albeit at different time points, in fish exposed to the metals mixture relative to those exposed to Cd alone, whereas Cu alone exposure increased Cd partitioning. Exposure to the metals mixture reduced (HDP) and enhanced (HSP, nuclei and granules) Cu accumulation while exposure to Zn alone enhanced Cu concentration in all the fractions analyzed without altering proportional distribution in MAP and MDP. Although subcellular Zn accumulation was less pronounced than that of either Cu or Cd, concentrations of Zn were enhanced in HDP, nuclei and granules from fish exposed to the metals mixture relative to those exposed to Zn alone. Cadmium alone exposure mobilized Zn and Cu from the nuclei and increased Zn accumulation in organelles and Cu in granules, while Cu alone exposure stimulated Zn accumulation in HSP, HDP and organelles. Interestingly, Cd alone exposure increased the partitioning of the three metals in MDP indicative of enhanced detoxification. Generally the accumulated metals were predominantly metabolically active: Cd, 67-83%; Cu, 68-79% and Zn, 60-76%. Taken together these results show both competitive and cooperative interactions dependent on the subcellular fraction, metal, exposure duration and relative metal exposure concentrations. Competitive interactions likely result from ionic mimicry with the metals displacing each other from common binding sites, whereas cooperative interactions suggest increased abundance of metal binding sites and/or existence of metal-specific non-interacting binding sites in some of the fractions. Moreover, the changes in subcellular distribution of the biometals Cu and Zn due to Cd exposure together with the shifts of the metals between MAP and MDP observed may have toxicological consequences.

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Publisher Full Text

Authors+Show Affiliations

Kamunde C
Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE, Canada. ckamunde@upei.ca
MacPhail R
No affiliation info available

MeSH

AnimalsBinding, CompetitiveCadmiumCopperDietDrug InteractionsHomeostasisInactivation, MetabolicLiverMetallothioneinMetals, HeavyOncorhynchus mykissRandom AllocationSubcellular FractionsWater Pollutants, ChemicalZinc

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21907009

Citation

Kamunde, Collins, and Ruth MacPhail. "Subcellular Interactions of Dietary Cadmium, Copper and Zinc in Rainbow Trout (Oncorhynchus Mykiss)." Aquatic Toxicology (Amsterdam, Netherlands), vol. 105, no. 3-4, 2011, pp. 518-27.
Kamunde C, MacPhail R. Subcellular interactions of dietary cadmium, copper and zinc in rainbow trout (Oncorhynchus mykiss). Aquat Toxicol. 2011;105(3-4):518-27.
Kamunde, C., & MacPhail, R. (2011). Subcellular interactions of dietary cadmium, copper and zinc in rainbow trout (Oncorhynchus mykiss). Aquatic Toxicology (Amsterdam, Netherlands), 105(3-4), 518-27. https://doi.org/10.1016/j.aquatox.2011.08.005
Kamunde C, MacPhail R. Subcellular Interactions of Dietary Cadmium, Copper and Zinc in Rainbow Trout (Oncorhynchus Mykiss). Aquat Toxicol. 2011;105(3-4):518-27. PubMed PMID: 21907009.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Subcellular interactions of dietary cadmium, copper and zinc in rainbow trout (Oncorhynchus mykiss). AU - Kamunde,Collins, AU - MacPhail,Ruth, Y1 - 2011/08/19/ PY - 2011/06/30/received PY - 2011/08/08/revised PY - 2011/08/11/accepted PY - 2011/9/13/entrez PY - 2011/9/13/pubmed PY - 2012/7/28/medline SP - 518 EP - 27 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 105 IS - 3-4 N2 - Interactions of Cu, Cd and Zn were studied at the subcellular level in juvenile rainbow trout (Oncorhynchus mykiss) fed diets containing (μg/g) 500 Cu, 1000 Zn and 500 Cd singly and as a ternary mixture for 28 days. Livers were harvested and submitted to differential centrifugation to isolate components of metabolically active metal pool (MAP: heat-denaturable proteins (HDP), organelles, nuclei) and metabolically detoxified metal pool (MDP: heat stable proteins (HSP), NaOH-resistant granules). Results indicated that Cd accumulation was enhanced in all the subcellular compartments, albeit at different time points, in fish exposed to the metals mixture relative to those exposed to Cd alone, whereas Cu alone exposure increased Cd partitioning. Exposure to the metals mixture reduced (HDP) and enhanced (HSP, nuclei and granules) Cu accumulation while exposure to Zn alone enhanced Cu concentration in all the fractions analyzed without altering proportional distribution in MAP and MDP. Although subcellular Zn accumulation was less pronounced than that of either Cu or Cd, concentrations of Zn were enhanced in HDP, nuclei and granules from fish exposed to the metals mixture relative to those exposed to Zn alone. Cadmium alone exposure mobilized Zn and Cu from the nuclei and increased Zn accumulation in organelles and Cu in granules, while Cu alone exposure stimulated Zn accumulation in HSP, HDP and organelles. Interestingly, Cd alone exposure increased the partitioning of the three metals in MDP indicative of enhanced detoxification. Generally the accumulated metals were predominantly metabolically active: Cd, 67-83%; Cu, 68-79% and Zn, 60-76%. Taken together these results show both competitive and cooperative interactions dependent on the subcellular fraction, metal, exposure duration and relative metal exposure concentrations. Competitive interactions likely result from ionic mimicry with the metals displacing each other from common binding sites, whereas cooperative interactions suggest increased abundance of metal binding sites and/or existence of metal-specific non-interacting binding sites in some of the fractions. Moreover, the changes in subcellular distribution of the biometals Cu and Zn due to Cd exposure together with the shifts of the metals between MAP and MDP observed may have toxicological consequences. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/21907009/Subcellular_interactions_of_dietary_cadmium_copper_and_zinc_in_rainbow_trout__Oncorhynchus_mykiss__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(11)00227-X DB - PRIME DP - Unbound Medicine ER -