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Electrospray ionization tandem mass spectrometry for the simultaneous determination of opiates and cocaine in human hair.
Anal Chim Acta. 2011 Oct 17; 704(1-2):123-32.AC

Abstract

A fast and highly sensitive electrospray ionization tandem mass spectrometry (ESI-MS/MS) method has been developed for the simultaneous determination of morphine, 6-methylacetylmorphine (6-MAM), codeine, cocaine and benzoylecgonine (BZE) in hair from drug abusers. Pulverized hair samples were subjected to an optimized matrix solid phase dispersion (MSPD) procedure with alumina, followed by diluted hydrochloric acid elution on column solid-phase extraction (SPE) clean-up/pre-concentration. Alternatively, samples were also subjected to an optimized ultrasound assisted enzymatic hydrolysis (USEH) with Pronase E, followed by an off-line SPE clean up/pre-concentration procedure. Positive electrospray ionization and multiple reaction monitoring (MRM) with one precursor ion/product ion transition were used for the identification and quantification (deuterated analogues of each target as internal standards) of each analyte. The chromatographic pump and the autosampler were used for injecting the standards and the hair extracts (20 μL) as a flow injection analysis mode. The highest sensitivity was achieved when delivering the targets with an acetonitrile/water/formic acid (80/19.875/0.125) mixture. The limits of detection of the method were 39.2, 4.4, 6.8, 7.0 and 7.4 ng g(-1) for morphine, 6-MAM, codeine, cocaine and BZE, respectively. Relative standard deviations of intra- and inter-day precision were lower than 9 and 12%, respectively; whereas, analytical recoveries ranged from 96±5 to 106±4%. The developed method (MSPD-ESI-MS/MS) was applied to different hair samples from polydrug abusers, and results were statistically compared to those obtained after a conventional gas chromatography-mass spectrometry (GC-MS) analysis and also after USEH and ESI-MS/MS or GC-MS determinations.

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Authors+Show Affiliations

Míguez-Framil M
Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, University of Santiago de Compostela, Santiago de Compostela, Spain.
Moreda-Piñeiro A
No affiliation info available
Bermejo-Barrera P
No affiliation info available
Cocho JÁ
No affiliation info available
Tabernero MJ
No affiliation info available
Bermejo AM
No affiliation info available

MeSH

Analgesics, OpioidChromatography, High Pressure LiquidCocaineFlow Injection AnalysisGas Chromatography-Mass SpectrometryHairHumansIllicit DrugsLimit of DetectionReproducibility of ResultsSolid Phase ExtractionSpectrometry, Mass, Electrospray IonizationSubstance Abuse DetectionTandem Mass Spectrometry

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21907029

Citation

Míguez-Framil, Martha, et al. "Electrospray Ionization Tandem Mass Spectrometry for the Simultaneous Determination of Opiates and Cocaine in Human Hair." Analytica Chimica Acta, vol. 704, no. 1-2, 2011, pp. 123-32.
Míguez-Framil M, Moreda-Piñeiro A, Bermejo-Barrera P, et al. Electrospray ionization tandem mass spectrometry for the simultaneous determination of opiates and cocaine in human hair. Anal Chim Acta. 2011;704(1-2):123-32.
Míguez-Framil, M., Moreda-Piñeiro, A., Bermejo-Barrera, P., Cocho, J. Á., Tabernero, M. J., & Bermejo, A. M. (2011). Electrospray ionization tandem mass spectrometry for the simultaneous determination of opiates and cocaine in human hair. Analytica Chimica Acta, 704(1-2), 123-32. https://doi.org/10.1016/j.aca.2011.08.013
Míguez-Framil M, et al. Electrospray Ionization Tandem Mass Spectrometry for the Simultaneous Determination of Opiates and Cocaine in Human Hair. Anal Chim Acta. 2011 Oct 17;704(1-2):123-32. PubMed PMID: 21907029.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrospray ionization tandem mass spectrometry for the simultaneous determination of opiates and cocaine in human hair. AU - Míguez-Framil,Martha, AU - Moreda-Piñeiro,Antonio, AU - Bermejo-Barrera,Pilar, AU - Cocho,José Ángel, AU - Tabernero,María Jesús, AU - Bermejo,Ana María, Y1 - 2011/08/11/ PY - 2011/05/19/received PY - 2011/07/19/revised PY - 2011/08/05/accepted PY - 2011/9/13/entrez PY - 2011/9/13/pubmed PY - 2012/1/11/medline SP - 123 EP - 32 JF - Analytica chimica acta JO - Anal Chim Acta VL - 704 IS - 1-2 N2 - A fast and highly sensitive electrospray ionization tandem mass spectrometry (ESI-MS/MS) method has been developed for the simultaneous determination of morphine, 6-methylacetylmorphine (6-MAM), codeine, cocaine and benzoylecgonine (BZE) in hair from drug abusers. Pulverized hair samples were subjected to an optimized matrix solid phase dispersion (MSPD) procedure with alumina, followed by diluted hydrochloric acid elution on column solid-phase extraction (SPE) clean-up/pre-concentration. Alternatively, samples were also subjected to an optimized ultrasound assisted enzymatic hydrolysis (USEH) with Pronase E, followed by an off-line SPE clean up/pre-concentration procedure. Positive electrospray ionization and multiple reaction monitoring (MRM) with one precursor ion/product ion transition were used for the identification and quantification (deuterated analogues of each target as internal standards) of each analyte. The chromatographic pump and the autosampler were used for injecting the standards and the hair extracts (20 μL) as a flow injection analysis mode. The highest sensitivity was achieved when delivering the targets with an acetonitrile/water/formic acid (80/19.875/0.125) mixture. The limits of detection of the method were 39.2, 4.4, 6.8, 7.0 and 7.4 ng g(-1) for morphine, 6-MAM, codeine, cocaine and BZE, respectively. Relative standard deviations of intra- and inter-day precision were lower than 9 and 12%, respectively; whereas, analytical recoveries ranged from 96±5 to 106±4%. The developed method (MSPD-ESI-MS/MS) was applied to different hair samples from polydrug abusers, and results were statistically compared to those obtained after a conventional gas chromatography-mass spectrometry (GC-MS) analysis and also after USEH and ESI-MS/MS or GC-MS determinations. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/21907029/Electrospray_ionization_tandem_mass_spectrometry_for_the_simultaneous_determination_of_opiates_and_cocaine_in_human_hair_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(11)01112-3 DB - PRIME DP - Unbound Medicine ER -