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Isolation and identification of phenolic compounds from rum aged in oak barrels by high-speed countercurrent chromatography/high-performance liquid chromatography-diode array detection-electrospray ionization mass spectrometry and screening for antioxidant activity.
J Chromatogr A. 2011 Oct 14; 1218(41):7358-64.JC

Abstract

Beverages, especially wines are well-known to contain a variety of health-beneficial bioactive substances, mainly of phenolic nature which frequently exhibit antioxidant activity. Significant information is available about the separation and identification of polyphenols from some beverages by chromatographic and spectroscopic techniques, but considerably poor is chemical data related to the polyphenolic content in rums. In this paper, a method involving the all-liquid chromatographic technique of high-speed countercurrent chromatography (HSCCC) combined with high-performance liquid chromatography coupled with diode-array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS(n)) has been successfully applied for separation and identification of phenolic compounds in an aged rum. Besides, the phenolic fraction (PF) was assayed for its antioxidant effects using three different free radical in vitro assays (DPPH·, RO(2)· and spontaneous lipid peroxidation (LPO) on brain homogenates) and on ferric reducing antioxidant power (FRAP). Results showed that PF potently scavenged DPPH and strongly scavenged peroxyl radicals compared to ascorbic acid and butylated hydroxytoluene (BHT); and almost equally inhibited LPO on brain homogenates subjected to spontaneous LPO when compared to quercetin. Moreover, PF also exhibited strong reducing power. This chemical analysis illustrates the rich array of phenols in the aged rum and represents a rapid and suitable method for the isolation and identification of phenolic compounds from mixtures of considerable complexity, achieving high purity and reproducibility with the use of two separation steps.

Authors+Show Affiliations

Université de Nice-Sophia Antipolis, Laboratoire de Chimie des Molécules Bioactives et des Arômes, UMR 6001 CNRS, Institut de Chimie de Nice, Faculté des Sciences, Nice, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21907994

Citation

Regalado, Erik L., et al. "Isolation and Identification of Phenolic Compounds From Rum Aged in Oak Barrels By High-speed Countercurrent Chromatography/high-performance Liquid Chromatography-diode Array Detection-electrospray Ionization Mass Spectrometry and Screening for Antioxidant Activity." Journal of Chromatography. A, vol. 1218, no. 41, 2011, pp. 7358-64.
Regalado EL, Tolle S, Pino JA, et al. Isolation and identification of phenolic compounds from rum aged in oak barrels by high-speed countercurrent chromatography/high-performance liquid chromatography-diode array detection-electrospray ionization mass spectrometry and screening for antioxidant activity. J Chromatogr A. 2011;1218(41):7358-64.
Regalado, E. L., Tolle, S., Pino, J. A., Winterhalter, P., Menendez, R., Morales, A. R., & Rodríguez, J. L. (2011). Isolation and identification of phenolic compounds from rum aged in oak barrels by high-speed countercurrent chromatography/high-performance liquid chromatography-diode array detection-electrospray ionization mass spectrometry and screening for antioxidant activity. Journal of Chromatography. A, 1218(41), 7358-64. https://doi.org/10.1016/j.chroma.2011.08.068
Regalado EL, et al. Isolation and Identification of Phenolic Compounds From Rum Aged in Oak Barrels By High-speed Countercurrent Chromatography/high-performance Liquid Chromatography-diode Array Detection-electrospray Ionization Mass Spectrometry and Screening for Antioxidant Activity. J Chromatogr A. 2011 Oct 14;1218(41):7358-64. PubMed PMID: 21907994.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and identification of phenolic compounds from rum aged in oak barrels by high-speed countercurrent chromatography/high-performance liquid chromatography-diode array detection-electrospray ionization mass spectrometry and screening for antioxidant activity. AU - Regalado,Erik L, AU - Tolle,Sebastian, AU - Pino,Jorge A, AU - Winterhalter,Peter, AU - Menendez,Roberto, AU - Morales,Ana R, AU - Rodríguez,José L, Y1 - 2011/08/25/ PY - 2011/06/22/received PY - 2011/08/16/revised PY - 2011/08/21/accepted PY - 2011/9/13/entrez PY - 2011/9/13/pubmed PY - 2011/12/13/medline SP - 7358 EP - 64 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1218 IS - 41 N2 - Beverages, especially wines are well-known to contain a variety of health-beneficial bioactive substances, mainly of phenolic nature which frequently exhibit antioxidant activity. Significant information is available about the separation and identification of polyphenols from some beverages by chromatographic and spectroscopic techniques, but considerably poor is chemical data related to the polyphenolic content in rums. In this paper, a method involving the all-liquid chromatographic technique of high-speed countercurrent chromatography (HSCCC) combined with high-performance liquid chromatography coupled with diode-array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS(n)) has been successfully applied for separation and identification of phenolic compounds in an aged rum. Besides, the phenolic fraction (PF) was assayed for its antioxidant effects using three different free radical in vitro assays (DPPH·, RO(2)· and spontaneous lipid peroxidation (LPO) on brain homogenates) and on ferric reducing antioxidant power (FRAP). Results showed that PF potently scavenged DPPH and strongly scavenged peroxyl radicals compared to ascorbic acid and butylated hydroxytoluene (BHT); and almost equally inhibited LPO on brain homogenates subjected to spontaneous LPO when compared to quercetin. Moreover, PF also exhibited strong reducing power. This chemical analysis illustrates the rich array of phenols in the aged rum and represents a rapid and suitable method for the isolation and identification of phenolic compounds from mixtures of considerable complexity, achieving high purity and reproducibility with the use of two separation steps. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/21907994/Isolation_and_identification_of_phenolic_compounds_from_rum_aged_in_oak_barrels_by_high_speed_countercurrent_chromatography/high_performance_liquid_chromatography_diode_array_detection_electrospray_ionization_mass_spectrometry_and_screening_for_antioxidant_activity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(11)01278-7 DB - PRIME DP - Unbound Medicine ER -