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Optimization, in-house validation, and application of a liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based method for the quantification of selected polyphenolic compounds in leaves of grapevine (Vitis vinifera L.).
J Agric Food Chem. 2011 Oct 26; 59(20):10787-94.JA

Abstract

Polyphenols in grapevine can be constitutive or induced, depending upon cultivar, plant organ, and environmental influences. The aim of the presented work was to develop and optimize a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to study the pattern and amount of selected polyphenols in leaves of Vitis vinifera L. The method is simple and does not require any sample cleanup. It covers representative metabolites of the structure classes cinnamic acids, flavonoids, and stilbenes and enables the simultaneous separation and quantification of 13 polyphenols within 9 min at concentration levels between 0.1 and 3 μg/g. We present the method performance characteristics and its application to the quantification of polyphenols in grapevine leaves of the cultivars Riesling and Pinot noir. A total of 7 of 13 target polyphenols were detected at concentrations above the limits of quantification. Interestingly, instead of the expected trans-resveratrol, the investigated leaf samples of both cultivars contained cis-resveratrol-3-O-glucoside. The measurements also showed that Riesling leaves tended to contain higher concentrations of the selected polyphenols than Pinot noir. In view of its intended future use, the developed method has been shown to be a powerful and fast tool to study polyphenols in grapevine leaves subjected to environmental stress conditions.

Authors+Show Affiliations

Division of Viticulture and Pomology, Department of Crop Sciences, University of Natural Resources and Life Sciences, Vienna, Konrad-Lorenz-Strasse 24, A-3430 Tulln, Austria.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

21910493

Citation

Schoedl, Katharina, et al. "Optimization, In-house Validation, and Application of a Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS)-based Method for the Quantification of Selected Polyphenolic Compounds in Leaves of Grapevine (Vitis Vinifera L.)." Journal of Agricultural and Food Chemistry, vol. 59, no. 20, 2011, pp. 10787-94.
Schoedl K, Forneck A, Sulyok M, et al. Optimization, in-house validation, and application of a liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based method for the quantification of selected polyphenolic compounds in leaves of grapevine (Vitis vinifera L.). J Agric Food Chem. 2011;59(20):10787-94.
Schoedl, K., Forneck, A., Sulyok, M., & Schuhmacher, R. (2011). Optimization, in-house validation, and application of a liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based method for the quantification of selected polyphenolic compounds in leaves of grapevine (Vitis vinifera L.). Journal of Agricultural and Food Chemistry, 59(20), 10787-94. https://doi.org/10.1021/jf202753g
Schoedl K, et al. Optimization, In-house Validation, and Application of a Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS)-based Method for the Quantification of Selected Polyphenolic Compounds in Leaves of Grapevine (Vitis Vinifera L.). J Agric Food Chem. 2011 Oct 26;59(20):10787-94. PubMed PMID: 21910493.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optimization, in-house validation, and application of a liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based method for the quantification of selected polyphenolic compounds in leaves of grapevine (Vitis vinifera L.). AU - Schoedl,Katharina, AU - Forneck,Astrid, AU - Sulyok,Michael, AU - Schuhmacher,Rainer, Y1 - 2011/09/26/ PY - 2011/9/14/entrez PY - 2011/9/14/pubmed PY - 2012/3/6/medline SP - 10787 EP - 94 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 59 IS - 20 N2 - Polyphenols in grapevine can be constitutive or induced, depending upon cultivar, plant organ, and environmental influences. The aim of the presented work was to develop and optimize a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to study the pattern and amount of selected polyphenols in leaves of Vitis vinifera L. The method is simple and does not require any sample cleanup. It covers representative metabolites of the structure classes cinnamic acids, flavonoids, and stilbenes and enables the simultaneous separation and quantification of 13 polyphenols within 9 min at concentration levels between 0.1 and 3 μg/g. We present the method performance characteristics and its application to the quantification of polyphenols in grapevine leaves of the cultivars Riesling and Pinot noir. A total of 7 of 13 target polyphenols were detected at concentrations above the limits of quantification. Interestingly, instead of the expected trans-resveratrol, the investigated leaf samples of both cultivars contained cis-resveratrol-3-O-glucoside. The measurements also showed that Riesling leaves tended to contain higher concentrations of the selected polyphenols than Pinot noir. In view of its intended future use, the developed method has been shown to be a powerful and fast tool to study polyphenols in grapevine leaves subjected to environmental stress conditions. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/21910493/Optimization_in_house_validation_and_application_of_a_liquid_chromatography_tandem_mass_spectrometry__LC_MS/MS__based_method_for_the_quantification_of_selected_polyphenolic_compounds_in_leaves_of_grapevine__Vitis_vinifera_L___ L2 - https://doi.org/10.1021/jf202753g DB - PRIME DP - Unbound Medicine ER -