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Hypoglycaemic activity and molecular mechanisms of Caesalpinia ferrea Martius bark extract on streptozotocin-induced diabetes in Wistar rats.
J Ethnopharmacol. 2011 Oct 11; 137(3):1533-41.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

The tea from the stem bark of Caesalpinia ferrea Martius (Leguminosae) has been popularly used in the treatment of diabetes in Brazil.

AIM OF THE STUDY

To investigate the hypoglycaemic properties and to elucidate the mechanisms by which the aqueous extract of the stem bark of Caesalpinia ferrea reduces blood glucose levels in streptozotocin-induced diabetic rats via the enzymatic pathways of protein kinase B (PKB/Akt), AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC).

MATERIALS AND METHODS

The aqueous extract of the stem bark of Caesalpinia ferrea (300 and 450 mg/kg/day), vehicle and metformin (500 mg/kg/day) were administered orally to STZ-diabetic rats (n = 7/group) for 4 weeks. Changes in body weight, food and water intake, fasting glucose levels and oral glucose tolerance were evaluated. Phosphorylation (P) and the expression of Akt, AMPK and ACC in the liver and skeletal muscle were determined using Western blot.

RESULTS

The aqueous extract of the stem bark of Caesalpinia ferrea reduced blood glucose levels and improved the metabolic state of the animals. P-Akt was increased in the liver and skeletal muscle of the treated animals, P-AMPK was reduced only in the skeletal muscle of these animals and P-ACC was reduced in both when compared with untreated rats.

CONCLUSION

The results indicate that the aqueous extract of the stem bark of Caesalpinia ferrea has hypoglycaemic properties and possibly acts to regulate glucose uptake in liver and muscles by way of Akt activation, restoring the intracellular energy balance confirmed by inhibition of AMPK activation.

Authors+Show Affiliations

Department of Pharmaceutical Sciences, Federal University of Pernambuco, Recife 50670-901, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21911047

Citation

Vasconcelos, C F B., et al. "Hypoglycaemic Activity and Molecular Mechanisms of Caesalpinia Ferrea Martius Bark Extract On Streptozotocin-induced Diabetes in Wistar Rats." Journal of Ethnopharmacology, vol. 137, no. 3, 2011, pp. 1533-41.
Vasconcelos CF, Maranhão HM, Batista TM, et al. Hypoglycaemic activity and molecular mechanisms of Caesalpinia ferrea Martius bark extract on streptozotocin-induced diabetes in Wistar rats. J Ethnopharmacol. 2011;137(3):1533-41.
Vasconcelos, C. F., Maranhão, H. M., Batista, T. M., Carneiro, E. M., Ferreira, F., Costa, J., Soares, L. A., Sá, M. D., Souza, T. P., & Wanderley, A. G. (2011). Hypoglycaemic activity and molecular mechanisms of Caesalpinia ferrea Martius bark extract on streptozotocin-induced diabetes in Wistar rats. Journal of Ethnopharmacology, 137(3), 1533-41. https://doi.org/10.1016/j.jep.2011.08.059
Vasconcelos CF, et al. Hypoglycaemic Activity and Molecular Mechanisms of Caesalpinia Ferrea Martius Bark Extract On Streptozotocin-induced Diabetes in Wistar Rats. J Ethnopharmacol. 2011 Oct 11;137(3):1533-41. PubMed PMID: 21911047.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hypoglycaemic activity and molecular mechanisms of Caesalpinia ferrea Martius bark extract on streptozotocin-induced diabetes in Wistar rats. AU - Vasconcelos,C F B, AU - Maranhão,H M L, AU - Batista,T M, AU - Carneiro,E M, AU - Ferreira,F, AU - Costa,J, AU - Soares,L A L, AU - Sá,M D C, AU - Souza,T P, AU - Wanderley,A G, Y1 - 2011/09/05/ PY - 2011/04/07/received PY - 2011/08/21/revised PY - 2011/08/25/accepted PY - 2011/9/14/entrez PY - 2011/9/14/pubmed PY - 2012/2/15/medline SP - 1533 EP - 41 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 137 IS - 3 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: The tea from the stem bark of Caesalpinia ferrea Martius (Leguminosae) has been popularly used in the treatment of diabetes in Brazil. AIM OF THE STUDY: To investigate the hypoglycaemic properties and to elucidate the mechanisms by which the aqueous extract of the stem bark of Caesalpinia ferrea reduces blood glucose levels in streptozotocin-induced diabetic rats via the enzymatic pathways of protein kinase B (PKB/Akt), AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC). MATERIALS AND METHODS: The aqueous extract of the stem bark of Caesalpinia ferrea (300 and 450 mg/kg/day), vehicle and metformin (500 mg/kg/day) were administered orally to STZ-diabetic rats (n = 7/group) for 4 weeks. Changes in body weight, food and water intake, fasting glucose levels and oral glucose tolerance were evaluated. Phosphorylation (P) and the expression of Akt, AMPK and ACC in the liver and skeletal muscle were determined using Western blot. RESULTS: The aqueous extract of the stem bark of Caesalpinia ferrea reduced blood glucose levels and improved the metabolic state of the animals. P-Akt was increased in the liver and skeletal muscle of the treated animals, P-AMPK was reduced only in the skeletal muscle of these animals and P-ACC was reduced in both when compared with untreated rats. CONCLUSION: The results indicate that the aqueous extract of the stem bark of Caesalpinia ferrea has hypoglycaemic properties and possibly acts to regulate glucose uptake in liver and muscles by way of Akt activation, restoring the intracellular energy balance confirmed by inhibition of AMPK activation. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/21911047/Hypoglycaemic_activity_and_molecular_mechanisms_of_Caesalpinia_ferrea_Martius_bark_extract_on_streptozotocin_induced_diabetes_in_Wistar_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(11)00632-5 DB - PRIME DP - Unbound Medicine ER -