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Effect of neferine on liver ischemia-reperfusion injury in rats.
Transplant Proc. 2011 Sep; 43(7):2536-9.TP

Abstract

INTRODUCTION

Liver ischemia/reperfusion leads to the formation of reactive oxygen species (ROS) that cause liver injury, a critical clinical problem during liver surgery and transplantation. The aim of the present study was to investigate the hepatoprotective and antioxidant effects of neferine against liver ischemia/reperfusion injury in rats.

MATERIALS AND METHODS

Wistar rats were randomly divided into 4 groups (n = 8): sham group; model group, and neferine high and low groups (50 and 25 mg/kg, respectively). After either saline or neferine was orally administered for 5 days rat livers were subjected to 30 minutes of ischemia followed by 6 hours of reperfusion. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), and hydroxyl radical levels were measured in serum. The liver was removed to assay malondialdehyde (MDA) and carbonyl contents, superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities, as well as to evaluate histopathologic changes.

RESULTS

Neferine significantly prevented AST and ALT elevations, reduced hydroxyl radical release, inhibited SOD and GPx activities, and decreased MDA and carbonyl contents. At the same time, neferine attenuated the histopathologic changes.

CONCLUSION

Neferine protected against liver ischemia/reperfusion in rats through antioxidant mechanisms. However, further studies are needed to verify whether the hepatoprotection of neferine is correlated with anti-inflammatory and anti-apoptotic effects.

Authors+Show Affiliations

First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21911119

Citation

Wang, J, et al. "Effect of Neferine On Liver Ischemia-reperfusion Injury in Rats." Transplantation Proceedings, vol. 43, no. 7, 2011, pp. 2536-9.
Wang J, Kan Q, Li J, et al. Effect of neferine on liver ischemia-reperfusion injury in rats. Transplant Proc. 2011;43(7):2536-9.
Wang, J., Kan, Q., Li, J., Zhang, X., & Qi, Y. (2011). Effect of neferine on liver ischemia-reperfusion injury in rats. Transplantation Proceedings, 43(7), 2536-9. https://doi.org/10.1016/j.transproceed.2011.04.013
Wang J, et al. Effect of Neferine On Liver Ischemia-reperfusion Injury in Rats. Transplant Proc. 2011;43(7):2536-9. PubMed PMID: 21911119.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of neferine on liver ischemia-reperfusion injury in rats. AU - Wang,J, AU - Kan,Q, AU - Li,J, AU - Zhang,X, AU - Qi,Y, PY - 2011/02/08/received PY - 2011/04/19/accepted PY - 2011/9/14/entrez PY - 2011/9/14/pubmed PY - 2012/1/11/medline SP - 2536 EP - 9 JF - Transplantation proceedings JO - Transplant Proc VL - 43 IS - 7 N2 - INTRODUCTION: Liver ischemia/reperfusion leads to the formation of reactive oxygen species (ROS) that cause liver injury, a critical clinical problem during liver surgery and transplantation. The aim of the present study was to investigate the hepatoprotective and antioxidant effects of neferine against liver ischemia/reperfusion injury in rats. MATERIALS AND METHODS: Wistar rats were randomly divided into 4 groups (n = 8): sham group; model group, and neferine high and low groups (50 and 25 mg/kg, respectively). After either saline or neferine was orally administered for 5 days rat livers were subjected to 30 minutes of ischemia followed by 6 hours of reperfusion. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), and hydroxyl radical levels were measured in serum. The liver was removed to assay malondialdehyde (MDA) and carbonyl contents, superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities, as well as to evaluate histopathologic changes. RESULTS: Neferine significantly prevented AST and ALT elevations, reduced hydroxyl radical release, inhibited SOD and GPx activities, and decreased MDA and carbonyl contents. At the same time, neferine attenuated the histopathologic changes. CONCLUSION: Neferine protected against liver ischemia/reperfusion in rats through antioxidant mechanisms. However, further studies are needed to verify whether the hepatoprotection of neferine is correlated with anti-inflammatory and anti-apoptotic effects. SN - 1873-2623 UR - https://www.unboundmedicine.com/medline/citation/21911119/Effect_of_neferine_on_liver_ischemia_reperfusion_injury_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-1345(11)00843-8 DB - PRIME DP - Unbound Medicine ER -