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193 nm ultraviolet photodissociation of deprotonated sialylated oligosaccharides.
Anal Chem. 2011 Nov 01; 83(21):8192-200.AC

Abstract

The fragmentation patterns of deprotonated sialylated oligosaccharides and glycans from fetuin obtained upon collisionally induced dissociation (CID) and 193 nm ultraviolet photodissociation (UVPD) in a linear ion trap are presented. UVPD produced a more extensive series of cross-ring cleavage ions, such as A- and X-type ions, and dual-cleavage internal ions, including A/Y and X/B fragment ions. In addition, UVPD generated unique fragment ions which arise from site-specific cleavage of the triol substituent of the sialic acid residue. In contrast, CID produced more conventional glycosidic cleavages and relatively few A-type ions. UVPD of doubly deprotonated sialylated oligosaccharides produced mostly singly deprotonated fragment ions, whereas the product ions in the CID spectra were overwhelmingly doubly charged ions, an outcome attributed to the more extensive cleavages of sialic acid residues upon UVPD and products from electron photodetachment. The larger array of product ions, including those arising from extensive cross-ring cleavages and dual-cleavage ions, generated by 193 nm UVPD relative to CID gives greater confidence for identification of glycans. Several key site-specific cleavages by UVPD, such as ones involving the sialic acid moieties, provide evidence of glycan composition.

Authors+Show Affiliations

Department of Chemical Engineering, University of Texas at Austin, Austin, Texas, United States.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

21913695

Citation

Ko, Byoung Joon, and Jennifer S. Brodbelt. "193 Nm Ultraviolet Photodissociation of Deprotonated Sialylated Oligosaccharides." Analytical Chemistry, vol. 83, no. 21, 2011, pp. 8192-200.
Ko BJ, Brodbelt JS. 193 nm ultraviolet photodissociation of deprotonated sialylated oligosaccharides. Anal Chem. 2011;83(21):8192-200.
Ko, B. J., & Brodbelt, J. S. (2011). 193 nm ultraviolet photodissociation of deprotonated sialylated oligosaccharides. Analytical Chemistry, 83(21), 8192-200. https://doi.org/10.1021/ac201751u
Ko BJ, Brodbelt JS. 193 Nm Ultraviolet Photodissociation of Deprotonated Sialylated Oligosaccharides. Anal Chem. 2011 Nov 1;83(21):8192-200. PubMed PMID: 21913695.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 193 nm ultraviolet photodissociation of deprotonated sialylated oligosaccharides. AU - Ko,Byoung Joon, AU - Brodbelt,Jennifer S, Y1 - 2011/10/03/ PY - 2011/9/15/entrez PY - 2011/9/15/pubmed PY - 2012/3/1/medline SP - 8192 EP - 200 JF - Analytical chemistry JO - Anal Chem VL - 83 IS - 21 N2 - The fragmentation patterns of deprotonated sialylated oligosaccharides and glycans from fetuin obtained upon collisionally induced dissociation (CID) and 193 nm ultraviolet photodissociation (UVPD) in a linear ion trap are presented. UVPD produced a more extensive series of cross-ring cleavage ions, such as A- and X-type ions, and dual-cleavage internal ions, including A/Y and X/B fragment ions. In addition, UVPD generated unique fragment ions which arise from site-specific cleavage of the triol substituent of the sialic acid residue. In contrast, CID produced more conventional glycosidic cleavages and relatively few A-type ions. UVPD of doubly deprotonated sialylated oligosaccharides produced mostly singly deprotonated fragment ions, whereas the product ions in the CID spectra were overwhelmingly doubly charged ions, an outcome attributed to the more extensive cleavages of sialic acid residues upon UVPD and products from electron photodetachment. The larger array of product ions, including those arising from extensive cross-ring cleavages and dual-cleavage ions, generated by 193 nm UVPD relative to CID gives greater confidence for identification of glycans. Several key site-specific cleavages by UVPD, such as ones involving the sialic acid moieties, provide evidence of glycan composition. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/21913695/193_nm_ultraviolet_photodissociation_of_deprotonated_sialylated_oligosaccharides_ L2 - https://doi.org/10.1021/ac201751u DB - PRIME DP - Unbound Medicine ER -