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Comparison of a rapid diagnostic test and microimmunofluorescence assay for detecting antibody to Orientia tsutsugamushi in scrub typhus patients in China.
Asian Pac J Trop Med. 2011 Aug; 4(8):666-8.AP

Abstract

OBJECTIVE

To evaluate the detection of IgM and IgG antibodies to Orientia tsutsugamushi (O. tsutsugamushi) by rapid diagnostic test (RDT) and microimmunofluorescence assay (mIFA).

METHODS

RDT using a mixture of recombinant 56-kDa proteins of O. tsutsugamushi and mIFA assay were performed on 20 patients from Fujian and 13 patients from Yunnan Province, and 82 sera samples from healthy farmers in Anhui Province and Beijing City in 2009. Comparison of the RDT and mIFA assay was performed by using X(2) test and the P level of <0.05 was considered to be significance.

RESULTS

Among these 82 normal sera samples, the specificity of RDT was 100% for both IgM and IgG tests. In 33 samples from patients with scrub typhus, 5 cases were positively detected earlier by RDT than by mIFA in IgM test, and 2 cases were positive in IgG test. Sensitivities of RDT were 93.9% and 90.9% for IgM and IgG, respectively. The sensitivity of combination test of IgM and IgG was 100%. Geometric mean titer diluted sera from confirmed cases by IFA and RDT assay were 1:37 vs. 1:113 (P<0.001) in IgM test and 1:99 vs. 1:279 (P<0.05) in IgG test.

CONCLUSIONS

RDT is more sensitivite than mIFA in the early diagnosis of scrub typhus and it is particularly applicable in rural areas.

Links

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Authors+Show Affiliations

Zhang L
Department of Rickettsiology, National Institute of Communicable Disease Control and Prevention, China CDC, Changping, Beijing, China. zhanglijuan@icdc.cn
He S
No affiliation info available
Wang S
No affiliation info available
Yu H
No affiliation info available
Li X
No affiliation info available
Zhang D
No affiliation info available
Pan L
No affiliation info available
Yu Q
No affiliation info available

MeSH

AdolescentAdultAgedAntibodies, BacterialBacterial ProteinsChildChinaDiagnostic Tests, RoutineFemaleFluorescent Antibody Technique, IndirectHumansImmunoglobulin GImmunoglobulin MMaleMiddle AgedOrientia tsutsugamushiRecombinant ProteinsScrub TyphusSensitivity and Specificity

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21914549

Citation

Zhang, Lijuan, et al. "Comparison of a Rapid Diagnostic Test and Microimmunofluorescence Assay for Detecting Antibody to Orientia Tsutsugamushi in Scrub Typhus Patients in China." Asian Pacific Journal of Tropical Medicine, vol. 4, no. 8, 2011, pp. 666-8.
Zhang L, He S, Wang S, et al. Comparison of a rapid diagnostic test and microimmunofluorescence assay for detecting antibody to Orientia tsutsugamushi in scrub typhus patients in China. Asian Pac J Trop Med. 2011;4(8):666-8.
Zhang, L., He, S., Wang, S., Yu, H., Li, X., Zhang, D., Pan, L., & Yu, Q. (2011). Comparison of a rapid diagnostic test and microimmunofluorescence assay for detecting antibody to Orientia tsutsugamushi in scrub typhus patients in China. Asian Pacific Journal of Tropical Medicine, 4(8), 666-8. https://doi.org/10.1016/S1995-7645(11)60169-7
Zhang L, et al. Comparison of a Rapid Diagnostic Test and Microimmunofluorescence Assay for Detecting Antibody to Orientia Tsutsugamushi in Scrub Typhus Patients in China. Asian Pac J Trop Med. 2011;4(8):666-8. PubMed PMID: 21914549.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of a rapid diagnostic test and microimmunofluorescence assay for detecting antibody to Orientia tsutsugamushi in scrub typhus patients in China. AU - Zhang,Lijuan, AU - He,Si, AU - Wang,Shiwen, AU - Yu,Huilan, AU - Li,Xuemei, AU - Zhang,Derong, AU - Pan,Lei, AU - Yu,Qiang, PY - 2011/04/11/received PY - 2011/06/11/revised PY - 2011/07/15/accepted PY - 2011/9/15/entrez PY - 2011/9/15/pubmed PY - 2012/1/28/medline SP - 666 EP - 8 JF - Asian Pacific journal of tropical medicine JO - Asian Pac J Trop Med VL - 4 IS - 8 N2 - OBJECTIVE: To evaluate the detection of IgM and IgG antibodies to Orientia tsutsugamushi (O. tsutsugamushi) by rapid diagnostic test (RDT) and microimmunofluorescence assay (mIFA). METHODS: RDT using a mixture of recombinant 56-kDa proteins of O. tsutsugamushi and mIFA assay were performed on 20 patients from Fujian and 13 patients from Yunnan Province, and 82 sera samples from healthy farmers in Anhui Province and Beijing City in 2009. Comparison of the RDT and mIFA assay was performed by using X(2) test and the P level of <0.05 was considered to be significance. RESULTS: Among these 82 normal sera samples, the specificity of RDT was 100% for both IgM and IgG tests. In 33 samples from patients with scrub typhus, 5 cases were positively detected earlier by RDT than by mIFA in IgM test, and 2 cases were positive in IgG test. Sensitivities of RDT were 93.9% and 90.9% for IgM and IgG, respectively. The sensitivity of combination test of IgM and IgG was 100%. Geometric mean titer diluted sera from confirmed cases by IFA and RDT assay were 1:37 vs. 1:113 (P<0.001) in IgM test and 1:99 vs. 1:279 (P<0.05) in IgG test. CONCLUSIONS: RDT is more sensitivite than mIFA in the early diagnosis of scrub typhus and it is particularly applicable in rural areas. SN - 2352-4146 UR - https://www.unboundmedicine.com/medline/citation/21914549/Comparison_of_a_rapid_diagnostic_test_and_microimmunofluorescence_assay_for_detecting_antibody_to_Orientia_tsutsugamushi_in_scrub_typhus_patients_in_China_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1995-7645(11)60169-7 DB - PRIME DP - Unbound Medicine ER -