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Fascin protein is critical for transforming growth factor β protein-induced invasion and filopodia formation in spindle-shaped tumor cells.
J Biol Chem. 2011 Nov 11; 286(45):38865-75.JB

Abstract

Fascin, an actin-bundling protein overexpressed in all carcinomas, has been associated with poor prognosis, shorter survival, and more metastatic diseases. It is believed that fascin facilitates tumor metastasis by promoting the formation of invasive membrane protrusions. However, the mechanisms by which fascin is overexpressed in tumors are not clear. TGFβ is a cytokine secreted by tumor and mesenchymal cells and promotes metastasis in many late stage tumors. The pro-metastasis mechanisms of TGFβ remain to be fully elucidated. Here we demonstrated that TGFβ induced fascin expression in spindle-shaped tumor cells through the canonical Smad-dependent pathway. Fascin was critical for TGFβ-promoted filopodia formation, migration, and invasion in spindle tumor cells. More importantly, fascin expression significantly correlates with TGFβ1 and TGFβ receptor I levels in a cohort of primary breast tumor samples. Our results indicate that elevated TGFβ level in the tumor microenvironment may be responsible for fascin overexpression in some of the metastatic tumors. Our data also suggest that fascin could play a central role in TGFβ-promoted tumor metastasis.

Authors+Show Affiliations

H. Lee Moffitt Cancer Center and Research Institute, Tampa, Florida 33612, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21914811

Citation

Sun, Jianwei, et al. "Fascin Protein Is Critical for Transforming Growth Factor Β Protein-induced Invasion and Filopodia Formation in Spindle-shaped Tumor Cells." The Journal of Biological Chemistry, vol. 286, no. 45, 2011, pp. 38865-75.
Sun J, He H, Xiong Y, et al. Fascin protein is critical for transforming growth factor β protein-induced invasion and filopodia formation in spindle-shaped tumor cells. J Biol Chem. 2011;286(45):38865-75.
Sun, J., He, H., Xiong, Y., Lu, S., Shen, J., Cheng, A., Chang, W. C., Hou, M. F., Lancaster, J. M., Kim, M., & Yang, S. (2011). Fascin protein is critical for transforming growth factor β protein-induced invasion and filopodia formation in spindle-shaped tumor cells. The Journal of Biological Chemistry, 286(45), 38865-75. https://doi.org/10.1074/jbc.M111.270413
Sun J, et al. Fascin Protein Is Critical for Transforming Growth Factor Β Protein-induced Invasion and Filopodia Formation in Spindle-shaped Tumor Cells. J Biol Chem. 2011 Nov 11;286(45):38865-75. PubMed PMID: 21914811.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fascin protein is critical for transforming growth factor β protein-induced invasion and filopodia formation in spindle-shaped tumor cells. AU - Sun,Jianwei, AU - He,Huifang, AU - Xiong,Yin, AU - Lu,Shuang, AU - Shen,Junling, AU - Cheng,Anna, AU - Chang,Wei-Chiao, AU - Hou,Ming-Feng, AU - Lancaster,Johnathan M, AU - Kim,Minjung, AU - Yang,Shengyu, Y1 - 2011/09/13/ PY - 2011/9/15/entrez PY - 2011/9/15/pubmed PY - 2011/12/28/medline SP - 38865 EP - 75 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 286 IS - 45 N2 - Fascin, an actin-bundling protein overexpressed in all carcinomas, has been associated with poor prognosis, shorter survival, and more metastatic diseases. It is believed that fascin facilitates tumor metastasis by promoting the formation of invasive membrane protrusions. However, the mechanisms by which fascin is overexpressed in tumors are not clear. TGFβ is a cytokine secreted by tumor and mesenchymal cells and promotes metastasis in many late stage tumors. The pro-metastasis mechanisms of TGFβ remain to be fully elucidated. Here we demonstrated that TGFβ induced fascin expression in spindle-shaped tumor cells through the canonical Smad-dependent pathway. Fascin was critical for TGFβ-promoted filopodia formation, migration, and invasion in spindle tumor cells. More importantly, fascin expression significantly correlates with TGFβ1 and TGFβ receptor I levels in a cohort of primary breast tumor samples. Our results indicate that elevated TGFβ level in the tumor microenvironment may be responsible for fascin overexpression in some of the metastatic tumors. Our data also suggest that fascin could play a central role in TGFβ-promoted tumor metastasis. SN - 1083-351X UR - https://www.unboundmedicine.com/medline/citation/21914811/Fascin_protein_is_critical_for_transforming_growth_factor_β_protein_induced_invasion_and_filopodia_formation_in_spindle_shaped_tumor_cells_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=21914811 DB - PRIME DP - Unbound Medicine ER -