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Impaired type I and III interferon response to rhinovirus infection during pregnancy and asthma.
Thorax 2012; 67(3):209-14T

Abstract

BACKGROUND

Acute respiratory tract infections are common ailments to all individuals and the human rhinoviruses (HRVs) cause most of these infections. Pregnant women have increased susceptibility and disease severity to viral infections like influenza and HRVs, as do asthmatics. Successful pregnancy requires immunological modulation to permit fetal tolerance.

OBJECTIVES

To determine whether pregnant women have reduced innate antiviral interferon (IFN) responses to HRV infection compared with non-pregnant women.

METHODS

An in vitro culture system was used, where peripheral blood mononuclear cells (PBMCs) were isolated from whole blood of 54 women, including 10 stable asthmatics who were pregnant and 10 who were not pregnant, 10 non-asthmatic women who were pregnant, 10 who were ≥6 months post partum and 10 who were not pregnant. Samples were also collected from four exacerbating pregnant asthmatics. PBMCs were cultured with HRV43 and HRV1B. The antiviral proteins IFNα and IFNλ were measured from culture supernatants by ELISA.

RESULTS

Compared with healthy non-pregnant women, pregnant women had significantly reduced innate IFN responses to HRV infection (p<0.02), persisting ≥6 months post partum (p≤0.02). Pregnant asthmatics had significantly reduced IFNλ responses compared with healthy non-pregnant women (p≤0.034), while during current asthma exacerbations a decrease in IFNα (p≤0.023) and IFNλ (p=0.007) was observed. Induction by a TLR7 agonist induced a similar pattern of decreased innate IFNs during pregnancy as observed when HRV was the inducing agent.

CONCLUSIONS

Reduced antiviral IFNs during pregnancy and asthma provide an important mechanism for increased susceptibility, morbidity and mortality in pregnant women with respiratory viral infection.

Authors+Show Affiliations

Department of Respiratory Medicine, Centre for Asthma and Respiratory Disease, The University of Newcastle, School of Medicine and Public Health , New Lambton, NSW, Australia, 2350. rebecca.l.forbes@uon.edu.auNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21917654

Citation

Forbes, Rebecca Louise, et al. "Impaired Type I and III Interferon Response to Rhinovirus Infection During Pregnancy and Asthma." Thorax, vol. 67, no. 3, 2012, pp. 209-14.
Forbes RL, Gibson PG, Murphy VE, et al. Impaired type I and III interferon response to rhinovirus infection during pregnancy and asthma. Thorax. 2012;67(3):209-14.
Forbes, R. L., Gibson, P. G., Murphy, V. E., & Wark, P. A. (2012). Impaired type I and III interferon response to rhinovirus infection during pregnancy and asthma. Thorax, 67(3), pp. 209-14. doi:10.1136/thoraxjnl-2011-200708.
Forbes RL, et al. Impaired Type I and III Interferon Response to Rhinovirus Infection During Pregnancy and Asthma. Thorax. 2012;67(3):209-14. PubMed PMID: 21917654.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impaired type I and III interferon response to rhinovirus infection during pregnancy and asthma. AU - Forbes,Rebecca Louise, AU - Gibson,Peter G, AU - Murphy,Vanessa E, AU - Wark,Peter A B, Y1 - 2011/09/13/ PY - 2011/9/16/entrez PY - 2011/9/16/pubmed PY - 2012/4/11/medline SP - 209 EP - 14 JF - Thorax JO - Thorax VL - 67 IS - 3 N2 - BACKGROUND: Acute respiratory tract infections are common ailments to all individuals and the human rhinoviruses (HRVs) cause most of these infections. Pregnant women have increased susceptibility and disease severity to viral infections like influenza and HRVs, as do asthmatics. Successful pregnancy requires immunological modulation to permit fetal tolerance. OBJECTIVES: To determine whether pregnant women have reduced innate antiviral interferon (IFN) responses to HRV infection compared with non-pregnant women. METHODS: An in vitro culture system was used, where peripheral blood mononuclear cells (PBMCs) were isolated from whole blood of 54 women, including 10 stable asthmatics who were pregnant and 10 who were not pregnant, 10 non-asthmatic women who were pregnant, 10 who were ≥6 months post partum and 10 who were not pregnant. Samples were also collected from four exacerbating pregnant asthmatics. PBMCs were cultured with HRV43 and HRV1B. The antiviral proteins IFNα and IFNλ were measured from culture supernatants by ELISA. RESULTS: Compared with healthy non-pregnant women, pregnant women had significantly reduced innate IFN responses to HRV infection (p<0.02), persisting ≥6 months post partum (p≤0.02). Pregnant asthmatics had significantly reduced IFNλ responses compared with healthy non-pregnant women (p≤0.034), while during current asthma exacerbations a decrease in IFNα (p≤0.023) and IFNλ (p=0.007) was observed. Induction by a TLR7 agonist induced a similar pattern of decreased innate IFNs during pregnancy as observed when HRV was the inducing agent. CONCLUSIONS: Reduced antiviral IFNs during pregnancy and asthma provide an important mechanism for increased susceptibility, morbidity and mortality in pregnant women with respiratory viral infection. SN - 1468-3296 UR - https://www.unboundmedicine.com/medline/citation/21917654/Impaired_type_I_and_III_interferon_response_to_rhinovirus_infection_during_pregnancy_and_asthma_ L2 - http://thorax.bmj.com/cgi/pmidlookup?view=long&amp;pmid=21917654 DB - PRIME DP - Unbound Medicine ER -