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Reliable detection of milk allergens in food using a high-resolution, stand-alone mass spectrometer.

Abstract

Reliable methods are needed for detection of allergenic milk proteins in complex food matrixes. The feasibility of an LC/high-resolution MS method for the analysis of milk proteins in a thermally processed model food (incurred cookies) and in white wine spiked, respectively, with milk powder and caseinate is described. Detection of milk proteins was based on the identification of unique peptides in the tryptic digests of cookie/wine extracts using an RP-HPLC separation coupled to an Exactive nonhybrid mass spectrometer using Orbitrap technology. The extremely high mass accuracy and resolution provided by the Orbitrap analyzer allowed a fast preliminary identification of four previously proposed peptide markers of caseins using only accurate values of the m/z of their ions. No interference was observed, despite the complexity of the analyzed matrixes. Moreover, the availability of a high- energy, collisionally activated dissociation cell integrated in the mass spectrometer enabled acquisition of peptide MS/MS-like spectra through post-source fragmentation. Confirmation of peptide marker identity could then be achieved by a comparison between experimental and predicted product ions. The described method shows the great potential of Orbitrap MS as a reliable technique in the field of protein allergen detection once the peptide markers are identified.

Authors+Show Affiliations

,

Institute of Sciences of Food Production, National Research Council of Italy (ISPA-CNR), Via Amendola 122/O, 70126, Bari, Italy. Linda.monaci@ispa.cnr.it

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Source

MeSH

Allergens
Animals
Food Analysis
Mass Spectrometry
Milk
Reproducibility of Results

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21919336

Citation

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TY - JOUR T1 - Reliable detection of milk allergens in food using a high-resolution, stand-alone mass spectrometer. AU - Monaci,Linda, AU - Losito,Ilario, AU - Palmisano,Francesco, AU - Visconti,Angelo, PY - 2011/9/17/entrez PY - 2011/9/17/pubmed PY - 2011/10/25/medline SP - 1034 EP - 42 JF - Journal of AOAC International JO - J AOAC Int VL - 94 IS - 4 N2 - Reliable methods are needed for detection of allergenic milk proteins in complex food matrixes. The feasibility of an LC/high-resolution MS method for the analysis of milk proteins in a thermally processed model food (incurred cookies) and in white wine spiked, respectively, with milk powder and caseinate is described. Detection of milk proteins was based on the identification of unique peptides in the tryptic digests of cookie/wine extracts using an RP-HPLC separation coupled to an Exactive nonhybrid mass spectrometer using Orbitrap technology. The extremely high mass accuracy and resolution provided by the Orbitrap analyzer allowed a fast preliminary identification of four previously proposed peptide markers of caseins using only accurate values of the m/z of their ions. No interference was observed, despite the complexity of the analyzed matrixes. Moreover, the availability of a high- energy, collisionally activated dissociation cell integrated in the mass spectrometer enabled acquisition of peptide MS/MS-like spectra through post-source fragmentation. Confirmation of peptide marker identity could then be achieved by a comparison between experimental and predicted product ions. The described method shows the great potential of Orbitrap MS as a reliable technique in the field of protein allergen detection once the peptide markers are identified. SN - 1060-3271 UR - https://www.unboundmedicine.com/medline/citation/21919336/Reliable_detection_of_milk_allergens_in_food_using_a_high_resolution_stand_alone_mass_spectrometer_ DB - PRIME DP - Unbound Medicine ER -