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Characterization of a new 4BS.7HL wheat-barley translocation line using GISH, FISH, and SSR markers and its effect on the β-glucan content of wheat.
Genome. 2011 Oct; 54(10):795-804.G

Abstract

A spontaneous interspecific Robertsonian translocation was revealed by genomic in situ hybridization (GISH) in the progenies of a monosomic 7H addition line originating from a new wheat 'Asakaze komugi' × barley 'Manas' hybrid. Fluorescence in situ hybridization (FISH) with repetitive DNA sequences (Afa family, pSc119.2, and pTa71) allowed identification of all wheat chromosomes, including wheat chromosome arm 4BS involved in the translocation. FISH using barley telomere- and centromere-specific repetitive DNA probes (HvT01 and (AGGGAG)(n)) confirmed that one of the arms of barley chromosome 7H was involved in the translocation. Simple sequence repeat (SSR) markers specific to the long (L) and short (S) arms of barley chromosome 7H identified the translocated chromosome segment as 7HL. Further analysis of the translocation chromosome clarified the physical position of genetically mapped SSRs within 7H, with a special focus on its centromeric region. The presence of the HvCslF6 gene, responsible for (1,3;1,4)-β-D-glucan production, was revealed in the centromeric region of 7HL. An increased (1,3;1,4)-β-D-glucan level was also detected in the translocation line, demonstrating that the HvCslF6 gene is of potential relevance for the manipulation of wheat (1,3;1,4)-β-D-glucan levels.

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Authors+Show Affiliations

Cseh A
Agricultural Research Institute of the Hungarian Academy of Sciences, Martonvásár, Hungary.
Kruppa K
No affiliation info available
Molnár I
No affiliation info available
Rakszegi M
No affiliation info available
Doležel J
No affiliation info available
Molnár-Láng M
No affiliation info available

MeSH

ChimeraChromosomes, PlantGenetic MarkersGenome, PlantHordeumIn Situ Hybridization, FluorescenceMicrosatellite RepeatsPlant ProteinsTranslocation, GeneticTriticumbeta-Glucans

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21919737

Citation

Cseh, A, et al. "Characterization of a New 4BS.7HL Wheat-barley Translocation Line Using GISH, FISH, and SSR Markers and Its Effect On the Β-glucan Content of Wheat." Genome, vol. 54, no. 10, 2011, pp. 795-804.
Cseh A, Kruppa K, Molnár I, et al. Characterization of a new 4BS.7HL wheat-barley translocation line using GISH, FISH, and SSR markers and its effect on the β-glucan content of wheat. Genome. 2011;54(10):795-804.
Cseh, A., Kruppa, K., Molnár, I., Rakszegi, M., Doležel, J., & Molnár-Láng, M. (2011). Characterization of a new 4BS.7HL wheat-barley translocation line using GISH, FISH, and SSR markers and its effect on the β-glucan content of wheat. Genome, 54(10), 795-804. https://doi.org/10.1139/g11-044
Cseh A, et al. Characterization of a New 4BS.7HL Wheat-barley Translocation Line Using GISH, FISH, and SSR Markers and Its Effect On the Β-glucan Content of Wheat. Genome. 2011;54(10):795-804. PubMed PMID: 21919737.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of a new 4BS.7HL wheat-barley translocation line using GISH, FISH, and SSR markers and its effect on the β-glucan content of wheat. AU - Cseh,A, AU - Kruppa,K, AU - Molnár,I, AU - Rakszegi,M, AU - Doležel,J, AU - Molnár-Láng,M, Y1 - 2011/09/15/ PY - 2011/9/17/entrez PY - 2011/9/17/pubmed PY - 2012/4/6/medline SP - 795 EP - 804 JF - Genome JO - Genome VL - 54 IS - 10 N2 - A spontaneous interspecific Robertsonian translocation was revealed by genomic in situ hybridization (GISH) in the progenies of a monosomic 7H addition line originating from a new wheat 'Asakaze komugi' × barley 'Manas' hybrid. Fluorescence in situ hybridization (FISH) with repetitive DNA sequences (Afa family, pSc119.2, and pTa71) allowed identification of all wheat chromosomes, including wheat chromosome arm 4BS involved in the translocation. FISH using barley telomere- and centromere-specific repetitive DNA probes (HvT01 and (AGGGAG)(n)) confirmed that one of the arms of barley chromosome 7H was involved in the translocation. Simple sequence repeat (SSR) markers specific to the long (L) and short (S) arms of barley chromosome 7H identified the translocated chromosome segment as 7HL. Further analysis of the translocation chromosome clarified the physical position of genetically mapped SSRs within 7H, with a special focus on its centromeric region. The presence of the HvCslF6 gene, responsible for (1,3;1,4)-β-D-glucan production, was revealed in the centromeric region of 7HL. An increased (1,3;1,4)-β-D-glucan level was also detected in the translocation line, demonstrating that the HvCslF6 gene is of potential relevance for the manipulation of wheat (1,3;1,4)-β-D-glucan levels. SN - 1480-3321 UR - https://www.unboundmedicine.com/medline/citation/21919737/Characterization_of_a_new_4BS_7HL_wheat_barley_translocation_line_using_GISH_FISH_and_SSR_markers_and_its_effect_on_the_β_glucan_content_of_wheat_ L2 - https://cdnsciencepub.com/doi/10.1139/g11-044?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -